Adrenergic signaling in human oral keratinocytes and wound repair

P. Steenhuis, R. E. Huntley, Z. Gurenko, L. Yin, B. A. Dale, Nasim Fazel, Roslyn Rivkah Isseroff

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Catecholamines are present in saliva, but their influence on oral epithelium is not understood. Because psychological stress increases salivary catecholamines and impairs oral mucosal wound healing, we sought to determine if epithelial adrenergic signaling could link these two findings. We found that cultured human oral keratinocytes (HOK) express the α 2B- and Î2-adrenergic receptors (ARs). Exposure of HOK to either epinephrine or the Î-AR agonist, isoproterenol, reduced migratory speed and decreased in vitro scratch wound healing. Incubation with the Î-AR antagonist timolol reversed the catecholamine-induced effects, indicating that the observed response is mediated by Î-AR. Epinephrine treatment decreased phosphorylation of the mitogen-activated protein kinases (MAPK) ERK1/2 and p38; these decreases were also reversed with timolol. Cultured HOK express enzymes of the epinephrine synthetic pathway, and generate epinephrine. These findings demonstrate that stress-induced elevations of salivary catecholamines signal through MAPK pathways, and result in impaired oral keratinocyte migration required for healing.

Original languageEnglish (US)
Pages (from-to)186-192
Number of pages7
JournalJournal of Dental Research
Volume90
Issue number2
DOIs
StatePublished - Feb 2011

Fingerprint

Keratinocytes
Adrenergic Agents
Epinephrine
Catecholamines
Timolol
Wounds and Injuries
Wound Healing
Adrenergic Receptors
Adrenergic Agonists
Adrenergic Antagonists
Mitogen-Activated Protein Kinase 1
p38 Mitogen-Activated Protein Kinases
Mitogen-Activated Protein Kinases
Isoproterenol
Saliva
Psychological Stress
Epithelium
Phosphorylation
Enzymes
Therapeutics

Keywords

  • adrenergic receptor
  • catecholamines
  • oral epithelium.
  • stress
  • wound repair

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Adrenergic signaling in human oral keratinocytes and wound repair. / Steenhuis, P.; Huntley, R. E.; Gurenko, Z.; Yin, L.; Dale, B. A.; Fazel, Nasim; Isseroff, Roslyn Rivkah.

In: Journal of Dental Research, Vol. 90, No. 2, 02.2011, p. 186-192.

Research output: Contribution to journalArticle

Steenhuis, P. ; Huntley, R. E. ; Gurenko, Z. ; Yin, L. ; Dale, B. A. ; Fazel, Nasim ; Isseroff, Roslyn Rivkah. / Adrenergic signaling in human oral keratinocytes and wound repair. In: Journal of Dental Research. 2011 ; Vol. 90, No. 2. pp. 186-192.
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