Abstract
In Study 1, semen was collected using a standardized electroejaculation procedure. Males (N = 8) produced ejaculates with a high incidence of sperm abnormalities (77 ± 3.3%). After electroejaculation under anaesthesia, serum cortisol concentrations increased (P < 0.05), while testosterone concentrations decreased (P < 0.05) and LH and FSH concentrations were unchanged (P > 0.05) over a 2-h bleeding period. In Study 2, male and female leopards were bled at 5-min intervals for 3 h and given (i.v.): (1) saline (N = 2/sex); (2) GnRH (1 μg/kg body weight) 30 min after the onset of sampling (N = 5/sex); or (3) ACTH (250 μg) at 30 min followed by GnRH 1 h later (N = 5/sex). Basal concentrations of serum LH, FSH and cortisol were comparable (P > 0.05) between male and female leopards. After GnRH, peak LH concentrations were 2-fold greater (P < 0.05) in males than females while FSH responses were similar. In males, testosterone concentrations increased 2-3-fold following GnRH. After ACTH, serum cortisol concentrations doubled within 15 min in both sexes. Administration of ACTH 1 h before GnRH did not affect GnRH-induced LH or FSH release (P > 0.05); however, testosterone secretion was only 30% of that observed after GnRH alone (P < 0.05). We conclude that (1) the high incidence of sperm abnormalities in the leopards of Sri Lanka may be related to parallel findings of genetic homozygosity; and (2) decreases in basal and GnRH-stimulated testosterone secretion were related to increases in serum cortisol afte electroejaculation or ACTH and were not associated with changes in pituitary gonadotrophin secretion.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 605-613 |
| Number of pages | 9 |
| Journal | Journal of Reproduction and Fertility |
| Volume | 85 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jan 1 1989 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Physiology
- Embryology
- Molecular Biology
- Obstetrics and Gynecology
- Developmental Biology
Cite this
Adrenal-pituitary-gonadal relationships and ejaculate characteristics in captive leopards (Panthera pardus kotiya) isolated on the island of Sri Lanka. / Brown, J. L.; Wildt, D. E.; Phillips, Lyndsay; Seidensticker, J.; Fernando, S. B.U.; Miththapala, S.; Goodrowe, K. L.
In: Journal of Reproduction and Fertility, Vol. 85, No. 2, 01.01.1989, p. 605-613.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Adrenal-pituitary-gonadal relationships and ejaculate characteristics in captive leopards (Panthera pardus kotiya) isolated on the island of Sri Lanka
AU - Brown, J. L.
AU - Wildt, D. E.
AU - Phillips, Lyndsay
AU - Seidensticker, J.
AU - Fernando, S. B.U.
AU - Miththapala, S.
AU - Goodrowe, K. L.
PY - 1989/1/1
Y1 - 1989/1/1
N2 - In Study 1, semen was collected using a standardized electroejaculation procedure. Males (N = 8) produced ejaculates with a high incidence of sperm abnormalities (77 ± 3.3%). After electroejaculation under anaesthesia, serum cortisol concentrations increased (P < 0.05), while testosterone concentrations decreased (P < 0.05) and LH and FSH concentrations were unchanged (P > 0.05) over a 2-h bleeding period. In Study 2, male and female leopards were bled at 5-min intervals for 3 h and given (i.v.): (1) saline (N = 2/sex); (2) GnRH (1 μg/kg body weight) 30 min after the onset of sampling (N = 5/sex); or (3) ACTH (250 μg) at 30 min followed by GnRH 1 h later (N = 5/sex). Basal concentrations of serum LH, FSH and cortisol were comparable (P > 0.05) between male and female leopards. After GnRH, peak LH concentrations were 2-fold greater (P < 0.05) in males than females while FSH responses were similar. In males, testosterone concentrations increased 2-3-fold following GnRH. After ACTH, serum cortisol concentrations doubled within 15 min in both sexes. Administration of ACTH 1 h before GnRH did not affect GnRH-induced LH or FSH release (P > 0.05); however, testosterone secretion was only 30% of that observed after GnRH alone (P < 0.05). We conclude that (1) the high incidence of sperm abnormalities in the leopards of Sri Lanka may be related to parallel findings of genetic homozygosity; and (2) decreases in basal and GnRH-stimulated testosterone secretion were related to increases in serum cortisol afte electroejaculation or ACTH and were not associated with changes in pituitary gonadotrophin secretion.
AB - In Study 1, semen was collected using a standardized electroejaculation procedure. Males (N = 8) produced ejaculates with a high incidence of sperm abnormalities (77 ± 3.3%). After electroejaculation under anaesthesia, serum cortisol concentrations increased (P < 0.05), while testosterone concentrations decreased (P < 0.05) and LH and FSH concentrations were unchanged (P > 0.05) over a 2-h bleeding period. In Study 2, male and female leopards were bled at 5-min intervals for 3 h and given (i.v.): (1) saline (N = 2/sex); (2) GnRH (1 μg/kg body weight) 30 min after the onset of sampling (N = 5/sex); or (3) ACTH (250 μg) at 30 min followed by GnRH 1 h later (N = 5/sex). Basal concentrations of serum LH, FSH and cortisol were comparable (P > 0.05) between male and female leopards. After GnRH, peak LH concentrations were 2-fold greater (P < 0.05) in males than females while FSH responses were similar. In males, testosterone concentrations increased 2-3-fold following GnRH. After ACTH, serum cortisol concentrations doubled within 15 min in both sexes. Administration of ACTH 1 h before GnRH did not affect GnRH-induced LH or FSH release (P > 0.05); however, testosterone secretion was only 30% of that observed after GnRH alone (P < 0.05). We conclude that (1) the high incidence of sperm abnormalities in the leopards of Sri Lanka may be related to parallel findings of genetic homozygosity; and (2) decreases in basal and GnRH-stimulated testosterone secretion were related to increases in serum cortisol afte electroejaculation or ACTH and were not associated with changes in pituitary gonadotrophin secretion.
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UR - http://www.scopus.com/inward/citedby.url?scp=0024590140&partnerID=8YFLogxK
U2 - 10.1530/jrf.0.0850605
DO - 10.1530/jrf.0.0850605
M3 - Article
C2 - 2539473
AN - SCOPUS:0024590140
VL - 85
SP - 605
EP - 613
JO - Journal of Reproduction and Fertility
JF - Journal of Reproduction and Fertility
SN - 0022-4251
IS - 2
ER -