Adoptive transfer of immunity to Nocardia asteroides in nude mice

R. L. Deem, B. L. Beaman, M. Eric Gershwin

Research output: Contribution to journalArticle

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Abstract

Nude mice on a BALB/c background were adoptively transferred with unprimed spleen cells, Nocardia-primed spleen cells, or Nocardia-primed splenic T lymphocytes from syngeneic, heterozygous (nu/+) littermates. Two days later, these recipient mice and unmanipulated (control) nude mice were infected intravenously with a 50% lethal dose of Nocardia asteroides GUH-2 from an early stationary-phase culture. Antibody titers, spleen weights, percent mortality, and organ clearance of the microorganisms were measured at 3 h to 28 days after infection. Adoptively transferred nude mice had larger spleens and greater titers of anti-nocardial antibody 7 to 28 days after infection as compared with control nude mice. Adoptive transfer with either primed spleen cells or primed splenic T lymphocytes enhanced both the survival of recipient nude mice and their ability to eliminate N. asteroides from the liver and spleen. These data indicate that adoptive immunity to infection with N. asteroides can be transferred with either specifically primed spleen cells or splenic T lymphocytes. Thus, it appears that cell-mediated immunity and T lymphocytes are of uppermost importance in host resistance to nocardial infection.

Original languageEnglish (US)
Pages (from-to)914-920
Number of pages7
JournalInfection and Immunity
Volume38
Issue number3
StatePublished - 1982

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Nocardia asteroides
Adoptive Transfer
Adaptive Immunity
Nude Mice
Spleen
T-Lymphocytes
Nocardia
Infection
Lethal Dose 50
Cellular Immunity
Anti-Idiotypic Antibodies
Weights and Measures
Mortality
Antibodies
Liver

ASJC Scopus subject areas

  • Immunology

Cite this

Adoptive transfer of immunity to Nocardia asteroides in nude mice. / Deem, R. L.; Beaman, B. L.; Gershwin, M. Eric.

In: Infection and Immunity, Vol. 38, No. 3, 1982, p. 914-920.

Research output: Contribution to journalArticle

Deem, R. L. ; Beaman, B. L. ; Gershwin, M. Eric. / Adoptive transfer of immunity to Nocardia asteroides in nude mice. In: Infection and Immunity. 1982 ; Vol. 38, No. 3. pp. 914-920.
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