Adipose fatty acid composition and rate of incorporation of α-linolenic acid differ between normal and lipoprotein lipase-deficient cats

Brian C. Veltri, Robert C. Backus, Quinton Rogers, Edward J. DePeters

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Normal adiposity occurs in humans and mice deficient of adipose lipoprotein lipase (LPL) activity. Subnormal adiposity found in LPL-deficient cats is indicative of limited de novo synthesis of fatty acids (FAs). In 14 LPL-deficient (3.0 ± 0.1 kg) and 8 normal (3.7 ± 0.1 kg) queens, FAs in triacylglycerol (TAG), phospholipid (PL), and nonesterified FAs (NEFAs) of plasma and inguinal subcutaneous adipose were determined before and after (d 38, 61, 110, 117, and 251) dietary linseed oil supplementation (30 g/kg). By d 60, LPL-deficient queens gained body weight (10.4 ± 0.1 kg), developed normal body fat mass (25 ± 2%), and were enriched in 18:3(n-3) in their plasma and adipose lipids. Adipose TAG 18:3(n-3) enrichment in LPL-deficient queens was subnormal at all sampling times and, as observed in normal queens, apparently not equilibrated by d 251. Adipose FA profiles in TAG but not PL were substantially different (P < 0.05) between LPL-deficient and normal queens; the 16:0 to 18:2(n-6) ratio was high in LPL-deficient (2.4-4.4) relative to normal queens (1.0-1.4). In LPL-deficient queens, fed-state plasma NEFA (n-6) and (n-3) enrichments were similar to those in adipose TAG, and plasma NEFA concentration was high (0.62 ± 0.05 mmol/L) and similar to that in normal queens after withholding diet for 16 h. These data indicate that LPL deficiency in cats reduces dietary FA storage efficiency, favors storage of saturated over unsaturated FAs, and stimulates de novo FA synthesis substantive enough to support normal adiposity.

Original languageEnglish (US)
Pages (from-to)2980-2986
Number of pages7
JournalJournal of Nutrition
Volume136
Issue number12
StatePublished - Dec 1 2006

Fingerprint

alpha-Linolenic Acid
lipoprotein lipase
Lipoprotein Lipase
linolenic acid
Cats
Fatty Acids
fatty acid composition
cats
Adiposity
Triglycerides
adiposity
triacylglycerols
fatty acids
Phospholipids
Hyperlipoproteinemia Type I
Linseed Oil
Unsaturated Dietary Fats
Groin
synthesis
linseed oil

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)

Cite this

Adipose fatty acid composition and rate of incorporation of α-linolenic acid differ between normal and lipoprotein lipase-deficient cats. / Veltri, Brian C.; Backus, Robert C.; Rogers, Quinton; DePeters, Edward J.

In: Journal of Nutrition, Vol. 136, No. 12, 01.12.2006, p. 2980-2986.

Research output: Contribution to journalArticle

@article{a3c2b04c03084cb48da826e523d84d51,
title = "Adipose fatty acid composition and rate of incorporation of α-linolenic acid differ between normal and lipoprotein lipase-deficient cats",
abstract = "Normal adiposity occurs in humans and mice deficient of adipose lipoprotein lipase (LPL) activity. Subnormal adiposity found in LPL-deficient cats is indicative of limited de novo synthesis of fatty acids (FAs). In 14 LPL-deficient (3.0 ± 0.1 kg) and 8 normal (3.7 ± 0.1 kg) queens, FAs in triacylglycerol (TAG), phospholipid (PL), and nonesterified FAs (NEFAs) of plasma and inguinal subcutaneous adipose were determined before and after (d 38, 61, 110, 117, and 251) dietary linseed oil supplementation (30 g/kg). By d 60, LPL-deficient queens gained body weight (10.4 ± 0.1 kg), developed normal body fat mass (25 ± 2{\%}), and were enriched in 18:3(n-3) in their plasma and adipose lipids. Adipose TAG 18:3(n-3) enrichment in LPL-deficient queens was subnormal at all sampling times and, as observed in normal queens, apparently not equilibrated by d 251. Adipose FA profiles in TAG but not PL were substantially different (P < 0.05) between LPL-deficient and normal queens; the 16:0 to 18:2(n-6) ratio was high in LPL-deficient (2.4-4.4) relative to normal queens (1.0-1.4). In LPL-deficient queens, fed-state plasma NEFA (n-6) and (n-3) enrichments were similar to those in adipose TAG, and plasma NEFA concentration was high (0.62 ± 0.05 mmol/L) and similar to that in normal queens after withholding diet for 16 h. These data indicate that LPL deficiency in cats reduces dietary FA storage efficiency, favors storage of saturated over unsaturated FAs, and stimulates de novo FA synthesis substantive enough to support normal adiposity.",
author = "Veltri, {Brian C.} and Backus, {Robert C.} and Quinton Rogers and DePeters, {Edward J.}",
year = "2006",
month = "12",
day = "1",
language = "English (US)",
volume = "136",
pages = "2980--2986",
journal = "Journal of Nutrition",
issn = "0022-3166",
publisher = "American Society for Nutrition",
number = "12",

}

TY - JOUR

T1 - Adipose fatty acid composition and rate of incorporation of α-linolenic acid differ between normal and lipoprotein lipase-deficient cats

AU - Veltri, Brian C.

AU - Backus, Robert C.

AU - Rogers, Quinton

AU - DePeters, Edward J.

PY - 2006/12/1

Y1 - 2006/12/1

N2 - Normal adiposity occurs in humans and mice deficient of adipose lipoprotein lipase (LPL) activity. Subnormal adiposity found in LPL-deficient cats is indicative of limited de novo synthesis of fatty acids (FAs). In 14 LPL-deficient (3.0 ± 0.1 kg) and 8 normal (3.7 ± 0.1 kg) queens, FAs in triacylglycerol (TAG), phospholipid (PL), and nonesterified FAs (NEFAs) of plasma and inguinal subcutaneous adipose were determined before and after (d 38, 61, 110, 117, and 251) dietary linseed oil supplementation (30 g/kg). By d 60, LPL-deficient queens gained body weight (10.4 ± 0.1 kg), developed normal body fat mass (25 ± 2%), and were enriched in 18:3(n-3) in their plasma and adipose lipids. Adipose TAG 18:3(n-3) enrichment in LPL-deficient queens was subnormal at all sampling times and, as observed in normal queens, apparently not equilibrated by d 251. Adipose FA profiles in TAG but not PL were substantially different (P < 0.05) between LPL-deficient and normal queens; the 16:0 to 18:2(n-6) ratio was high in LPL-deficient (2.4-4.4) relative to normal queens (1.0-1.4). In LPL-deficient queens, fed-state plasma NEFA (n-6) and (n-3) enrichments were similar to those in adipose TAG, and plasma NEFA concentration was high (0.62 ± 0.05 mmol/L) and similar to that in normal queens after withholding diet for 16 h. These data indicate that LPL deficiency in cats reduces dietary FA storage efficiency, favors storage of saturated over unsaturated FAs, and stimulates de novo FA synthesis substantive enough to support normal adiposity.

AB - Normal adiposity occurs in humans and mice deficient of adipose lipoprotein lipase (LPL) activity. Subnormal adiposity found in LPL-deficient cats is indicative of limited de novo synthesis of fatty acids (FAs). In 14 LPL-deficient (3.0 ± 0.1 kg) and 8 normal (3.7 ± 0.1 kg) queens, FAs in triacylglycerol (TAG), phospholipid (PL), and nonesterified FAs (NEFAs) of plasma and inguinal subcutaneous adipose were determined before and after (d 38, 61, 110, 117, and 251) dietary linseed oil supplementation (30 g/kg). By d 60, LPL-deficient queens gained body weight (10.4 ± 0.1 kg), developed normal body fat mass (25 ± 2%), and were enriched in 18:3(n-3) in their plasma and adipose lipids. Adipose TAG 18:3(n-3) enrichment in LPL-deficient queens was subnormal at all sampling times and, as observed in normal queens, apparently not equilibrated by d 251. Adipose FA profiles in TAG but not PL were substantially different (P < 0.05) between LPL-deficient and normal queens; the 16:0 to 18:2(n-6) ratio was high in LPL-deficient (2.4-4.4) relative to normal queens (1.0-1.4). In LPL-deficient queens, fed-state plasma NEFA (n-6) and (n-3) enrichments were similar to those in adipose TAG, and plasma NEFA concentration was high (0.62 ± 0.05 mmol/L) and similar to that in normal queens after withholding diet for 16 h. These data indicate that LPL deficiency in cats reduces dietary FA storage efficiency, favors storage of saturated over unsaturated FAs, and stimulates de novo FA synthesis substantive enough to support normal adiposity.

UR - http://www.scopus.com/inward/record.url?scp=33845317621&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33845317621&partnerID=8YFLogxK

M3 - Article

C2 - 17116707

AN - SCOPUS:33845317621

VL - 136

SP - 2980

EP - 2986

JO - Journal of Nutrition

JF - Journal of Nutrition

SN - 0022-3166

IS - 12

ER -