Adipose depot-specific effects of ileal interposition surgery in UCD-T2D rats

unexpected implications for obesity and diabetes

Connie Hung, Casey Bronec, Eleonora Napoli, James Graham, Kimber Stanhope, Ilaria Marsilio, Maria Cecilia Giron, Peter J Havel, Cecilia R Giulivi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Ileal interposition (IT) surgery delays the onset of diabetes in a rat model of type-2 diabetes (UCD-T2DM). Here, to gain a deeper understanding of the molecular events underlying the effects of IT surgery, we examined the changes in the proteome of four white adipose depots (retroperitoneal, mesenteric, inguinal, and epididymal) and plasma-free fatty acid profile in pre-diabetic rats 1.5 months following IT or sham surgery. The IT-mediated changes were exerted mainly in mesenteric fat and spanned from delayed adipocyte maturation to a neuroendocrine remodeling. Conversely, inguinal, retroperitoneal, and epididymal depots showed opposite trends consistent with increased adipocyte maturation and adipogenesis development prior to overt signs of diabetes, probably orchestrated by peroxisome proliferator-activated receptor gamma signaling and higher plasma n-6/n-3 free fatty acid ratios. The resulting scenario suggests a targeted use of surgical strategies that seek to delay or improve diabetes in order to manipulate adipose depot-specific responses to maximize the duration and beneficial effects of the surgery.

Original languageEnglish (US)
Pages (from-to)649-662
Number of pages14
JournalBiochemical Journal
Volume475
Issue number3
DOIs
StatePublished - Feb 14 2018

Fingerprint

Medical problems
Surgery
Rats
Obesity
Groin
Nonesterified Fatty Acids
Adipocytes
Plasmas
Adipogenesis
PPAR gamma
Proteome
Type 2 Diabetes Mellitus
Fats

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Adipose depot-specific effects of ileal interposition surgery in UCD-T2D rats : unexpected implications for obesity and diabetes. / Hung, Connie; Bronec, Casey; Napoli, Eleonora; Graham, James; Stanhope, Kimber; Marsilio, Ilaria; Giron, Maria Cecilia; Havel, Peter J; Giulivi, Cecilia R.

In: Biochemical Journal, Vol. 475, No. 3, 14.02.2018, p. 649-662.

Research output: Contribution to journalArticle

Hung, Connie ; Bronec, Casey ; Napoli, Eleonora ; Graham, James ; Stanhope, Kimber ; Marsilio, Ilaria ; Giron, Maria Cecilia ; Havel, Peter J ; Giulivi, Cecilia R. / Adipose depot-specific effects of ileal interposition surgery in UCD-T2D rats : unexpected implications for obesity and diabetes. In: Biochemical Journal. 2018 ; Vol. 475, No. 3. pp. 649-662.
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