Adhesion to fibronectin maintains regenerative capacity during ex vivo culture and transduction of human hematopoietic stem and progenitor cells

M. A. Dao, K. Hashino, I. Kato, Jan Nolta

Research output: Contribution to journalArticle

146 Scopus citations

Abstract

Recent reports have indicated that there is poor engraftment from hematopoietic stem cells (HSC) that have traversed cell cycle ex vivo. However, inducing cells to cycle in culture is critical to the fields of ex vivo stem cell expansion and retrovital-mediated gene therapy. Through the use of a xenograft model, the current data shows that human hematopoietic stem and progenitor cells can traverse M phase ex vivo, integrate retroviral vectors, engraft, and sustain long-term hematopoiesis only if they have had the opportunity to engage their integrin receptors to fibronectin during the culture period. If cultured in suspension under the same conditions, transduction is undetectable and the long-term multilineage regenerative capacity of the primitive cells is severely diminished.

Original languageEnglish (US)
Pages (from-to)4612-4621
Number of pages10
JournalBlood
Volume92
Issue number12
StatePublished - Dec 15 1998
Externally publishedYes

ASJC Scopus subject areas

  • Hematology

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