Abstract
Recent reports have indicated that there is poor engraftment from hematopoietic stem cells (HSC) that have traversed cell cycle ex vivo. However, inducing cells to cycle in culture is critical to the fields of ex vivo stem cell expansion and retrovital-mediated gene therapy. Through the use of a xenograft model, the current data shows that human hematopoietic stem and progenitor cells can traverse M phase ex vivo, integrate retroviral vectors, engraft, and sustain long-term hematopoiesis only if they have had the opportunity to engage their integrin receptors to fibronectin during the culture period. If cultured in suspension under the same conditions, transduction is undetectable and the long-term multilineage regenerative capacity of the primitive cells is severely diminished.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 4612-4621 |
| Number of pages | 10 |
| Journal | Blood |
| Volume | 92 |
| Issue number | 12 |
| State | Published - Dec 15 1998 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Hematology
Cite this
Adhesion to fibronectin maintains regenerative capacity during ex vivo culture and transduction of human hematopoietic stem and progenitor cells. / Dao, M. A.; Hashino, K.; Kato, I.; Nolta, Jan.
In: Blood, Vol. 92, No. 12, 15.12.1998, p. 4612-4621.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Adhesion to fibronectin maintains regenerative capacity during ex vivo culture and transduction of human hematopoietic stem and progenitor cells
AU - Dao, M. A.
AU - Hashino, K.
AU - Kato, I.
AU - Nolta, Jan
PY - 1998/12/15
Y1 - 1998/12/15
N2 - Recent reports have indicated that there is poor engraftment from hematopoietic stem cells (HSC) that have traversed cell cycle ex vivo. However, inducing cells to cycle in culture is critical to the fields of ex vivo stem cell expansion and retrovital-mediated gene therapy. Through the use of a xenograft model, the current data shows that human hematopoietic stem and progenitor cells can traverse M phase ex vivo, integrate retroviral vectors, engraft, and sustain long-term hematopoiesis only if they have had the opportunity to engage their integrin receptors to fibronectin during the culture period. If cultured in suspension under the same conditions, transduction is undetectable and the long-term multilineage regenerative capacity of the primitive cells is severely diminished.
AB - Recent reports have indicated that there is poor engraftment from hematopoietic stem cells (HSC) that have traversed cell cycle ex vivo. However, inducing cells to cycle in culture is critical to the fields of ex vivo stem cell expansion and retrovital-mediated gene therapy. Through the use of a xenograft model, the current data shows that human hematopoietic stem and progenitor cells can traverse M phase ex vivo, integrate retroviral vectors, engraft, and sustain long-term hematopoiesis only if they have had the opportunity to engage their integrin receptors to fibronectin during the culture period. If cultured in suspension under the same conditions, transduction is undetectable and the long-term multilineage regenerative capacity of the primitive cells is severely diminished.
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UR - http://www.scopus.com/inward/citedby.url?scp=0032535694&partnerID=8YFLogxK
M3 - Article
C2 - 9845527
AN - SCOPUS:0032535694
VL - 92
SP - 4612
EP - 4621
JO - Blood
JF - Blood
SN - 0006-4971
IS - 12
ER -