TY - JOUR
T1 - Adhesion and proliferation of corneal epithelial cells on self-assembled monolayers
AU - Franco, Marisol
AU - Nealey, Paul F.
AU - Campbell, Sean
AU - Teixeira, Ana I.
AU - Murphy, Christopher J
PY - 2000/11
Y1 - 2000/11
N2 - The effect of surface chemistry on the proliferation and adhesion of SV-40 human corneal epithelial cells was investigated. The surface chemistry of substrates was controlled by the deposition of self-assembled monolayers (SAMs) terminated with the following functional groups: -CF3, -CH3, -CO2H, and -NH2. SAMs of alkanethiols on gold and of alkylsiloxanes on SiOx were included in the study. Comparisons are made between different types and functionalities of SAMs and between SAM-covered substrates and tissue culture polystyrene. Adhesion assays were performed after incubation of the cells for 1 h in 10% fetal bovine serum and in serum-free conditions. The cellular response was found to be a function of surface chemistry and the presence of exogenous proteins. The number of cells that adhered to most of the SAMs in 10%-serum and in serum free conditions was not significantly different from the number of cells that adhered to TCPS. Proliferation assays were carried out in 10% serum and in 0.5% Serum. Cell behavior was influenced by surface Chemistry but did not deviate significantly from the behavior on TCPS for most of the SAMs. Serum level did not play a major role in cell proliferation. Our data establish the expected behaviors for a corneal epithelial cell line under defined conditions on specific surfaces. (C) 2000 John Wiley and Sons, Inc.
AB - The effect of surface chemistry on the proliferation and adhesion of SV-40 human corneal epithelial cells was investigated. The surface chemistry of substrates was controlled by the deposition of self-assembled monolayers (SAMs) terminated with the following functional groups: -CF3, -CH3, -CO2H, and -NH2. SAMs of alkanethiols on gold and of alkylsiloxanes on SiOx were included in the study. Comparisons are made between different types and functionalities of SAMs and between SAM-covered substrates and tissue culture polystyrene. Adhesion assays were performed after incubation of the cells for 1 h in 10% fetal bovine serum and in serum-free conditions. The cellular response was found to be a function of surface chemistry and the presence of exogenous proteins. The number of cells that adhered to most of the SAMs in 10%-serum and in serum free conditions was not significantly different from the number of cells that adhered to TCPS. Proliferation assays were carried out in 10% serum and in 0.5% Serum. Cell behavior was influenced by surface Chemistry but did not deviate significantly from the behavior on TCPS for most of the SAMs. Serum level did not play a major role in cell proliferation. Our data establish the expected behaviors for a corneal epithelial cell line under defined conditions on specific surfaces. (C) 2000 John Wiley and Sons, Inc.
KW - Adhesion
KW - Cell-surface interactions
KW - Corneal epithelial cells
KW - Proliferation
KW - Self-assembled monolayers
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U2 - 10.1002/1097-4636(200011)52:2<261::AID-JBM4>3.0.CO;2-2
DO - 10.1002/1097-4636(200011)52:2<261::AID-JBM4>3.0.CO;2-2
M3 - Article
C2 - 10951364
AN - SCOPUS:0034333623
VL - 52
SP - 261
EP - 269
JO - Journal of Biomedical Materials Research - Part A
JF - Journal of Biomedical Materials Research - Part A
SN - 1549-3296
IS - 2
ER -