Adhesion and proliferation of corneal epithelial cells on self-assembled monolayers

Marisol Franco, Paul F. Nealey, Sean Campbell, Ana I. Teixeira, Christopher J Murphy

Research output: Contribution to journalArticle

65 Scopus citations

Abstract

The effect of surface chemistry on the proliferation and adhesion of SV-40 human corneal epithelial cells was investigated. The surface chemistry of substrates was controlled by the deposition of self-assembled monolayers (SAMs) terminated with the following functional groups: -CF3, -CH3, -CO2H, and -NH2. SAMs of alkanethiols on gold and of alkylsiloxanes on SiOx were included in the study. Comparisons are made between different types and functionalities of SAMs and between SAM-covered substrates and tissue culture polystyrene. Adhesion assays were performed after incubation of the cells for 1 h in 10% fetal bovine serum and in serum-free conditions. The cellular response was found to be a function of surface chemistry and the presence of exogenous proteins. The number of cells that adhered to most of the SAMs in 10%-serum and in serum free conditions was not significantly different from the number of cells that adhered to TCPS. Proliferation assays were carried out in 10% serum and in 0.5% Serum. Cell behavior was influenced by surface Chemistry but did not deviate significantly from the behavior on TCPS for most of the SAMs. Serum level did not play a major role in cell proliferation. Our data establish the expected behaviors for a corneal epithelial cell line under defined conditions on specific surfaces. (C) 2000 John Wiley and Sons, Inc.

Original languageEnglish (US)
Pages (from-to)261-269
Number of pages9
JournalJournal of Biomedical Materials Research
Volume52
Issue number2
DOIs
StatePublished - Nov 2000
Externally publishedYes

Keywords

  • Adhesion
  • Cell-surface interactions
  • Corneal epithelial cells
  • Proliferation
  • Self-assembled monolayers

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

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