Adenosine prevents cardioplegia-induced Ca2+ loading in cardiac cells

A. Jovanovic, A. Alexeey, J. López, W. Shen, A. Terzic

Research output: Contribution to journalArticle

Abstract

Hyperkalemic cardioplegia is used to arrest the heart during open heart surgery by depolarizing the sarcolemma. A recognized adverse effect of hyperkalemic cardioplegia is ventricular dysfunction related to intracellular Ca2+ loading, but no effective means of protection under these conditions have been established. Whether adenosine, a known cardioprotective agent, could prevent intracellular Ca2+ loading without altering cardioplegia-induced depolarization was investigated using epifluorescent and laser confocal microscopy, and perforated patch-clamp electrophysiology. In ventricular myocytes isolated from guinea pig hearts, and loaded with a Ca2+-sensitive fluorescent probe, exposure of myocytes to 16 mM K+ induced intracellular Ca2+ loading, and membrane depolarization. Yet, adenosine (1 mM) effectively prevented Ca2+ loading whithout reducing the magnitude of cardioplegia-induced depolarization. The preventing effect of adenosine on Ca2+ loading was antagonized by inhibitors of protein kinase C. Adenosine did slow the rate of K+-induced membrane depolarization which could be repsonsible for a decrease in net Ca2+ influx. The property of adenosine to prevent hyperkalemia-induced Ca2+ loading may contribute to the cytoprotective efficacy of this agent as an adjunct to conventional hyperkalemic cardioplegia used in cardiac surgery.

Original languageEnglish (US)
Pages (from-to)163
Number of pages1
JournalClinical Pharmacology and Therapeutics
Volume61
Issue number2
StatePublished - 1997
Externally publishedYes

Fingerprint

Induced Heart Arrest
Adenosine
Confocal Microscopy
Muscle Cells
Thoracic Surgery
Cardiotonic Agents
Ventricular Dysfunction
Sarcolemma
Hyperkalemia
Membranes
Electrophysiology
Heart Arrest
Fluorescent Dyes
Protein Kinase C
Guinea Pigs

ASJC Scopus subject areas

  • Pharmacology

Cite this

Jovanovic, A., Alexeey, A., López, J., Shen, W., & Terzic, A. (1997). Adenosine prevents cardioplegia-induced Ca2+ loading in cardiac cells. Clinical Pharmacology and Therapeutics, 61(2), 163.

Adenosine prevents cardioplegia-induced Ca2+ loading in cardiac cells. / Jovanovic, A.; Alexeey, A.; López, J.; Shen, W.; Terzic, A.

In: Clinical Pharmacology and Therapeutics, Vol. 61, No. 2, 1997, p. 163.

Research output: Contribution to journalArticle

Jovanovic, A, Alexeey, A, López, J, Shen, W & Terzic, A 1997, 'Adenosine prevents cardioplegia-induced Ca2+ loading in cardiac cells', Clinical Pharmacology and Therapeutics, vol. 61, no. 2, pp. 163.
Jovanovic, A. ; Alexeey, A. ; López, J. ; Shen, W. ; Terzic, A. / Adenosine prevents cardioplegia-induced Ca2+ loading in cardiac cells. In: Clinical Pharmacology and Therapeutics. 1997 ; Vol. 61, No. 2. pp. 163.
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