Adenosine deaminase levels, serologic parameters, culture results, and polymerase chain reaction testing in pleural fluid

George Richard Thompson, Shobha Sharma, Derek J. Bays, Rachel Pruitt, David M. Engelthaler, Jolene Bowers, Elizabeth M. Driebe, Michael Davis, Robert Libke, Stuart H Cohen, Demosthenes Pappagianis

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Background: In a patient with positive serum serology for coccidioidomycosis, the differential diagnosis of concurrent pleural effusions can be challenging. We, therefore, sought to clarify the performance characteristics of biochemical, serologic, and nucleic-acid-based testing in an attempt to avoid invasive procedures. The utility of adenosine deaminase (ADA), coccidioidal serology, and polymerase chain reaction (PCR) in the evaluation of pleuropulmonary coccidioidomycosis has not been previously reported. Methods: Forty consecutive patients evaluated for pleuropulmonary coccidioidomycosis were included. Demographic data, pleural fluid values, culture results, and clinical diagnoses were obtained from patient chart review. ADA testing was performed by ARUP Laboratories, coccidioidal serologic testing was performed by the University of California-Davis coccidioidomycosis serology laboratory, and PCR testing was performed by the Translational Genomics Research Institute using a previously published methodology. Results: Fifteen patients were diagnosed with pleuropulmonary coccidioidomycosis by European Organization for the Research and Treatment of Cancer/Mycoses Study Group criteria. Pleural fluid ADA concentrations were <40 IU/L in all patients (range, <1.0-28.6 IU/L; median, 4.7). The sensitivity and specificity of coccidioidal serologic testing was 100% in this study. The specificity of PCR testing was high (100%), although the overall sensitivity remained low, and was comparable to the experience of others in the clinical use of PCR for coccidioidal diagnostics. Conclusion: Contrary to prior speculation, ADA levels in pleuropulmonary coccidioidomycosis were not elevated in this study. The sensitivity and specificity of coccidioidal serologic testing in nonserum samples remained high, but the clinical usefulness of PCR testing in pleural fluid was disappointing and was comparable to pleural fluid culture.

Original languageEnglish (US)
Pages (from-to)776-781
Number of pages6
JournalChest
Volume143
Issue number3
DOIs
StatePublished - Mar 2013

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Coccidioidomycosis
Adenosine Deaminase
Polymerase Chain Reaction
Serology
Sensitivity and Specificity
Translational Medical Research
Mycoses
Pleural Effusion
Genomics
Nucleic Acids
Differential Diagnosis
Demography
Organizations
Serum
Research
Neoplasms

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Critical Care and Intensive Care Medicine
  • Cardiology and Cardiovascular Medicine

Cite this

Adenosine deaminase levels, serologic parameters, culture results, and polymerase chain reaction testing in pleural fluid. / Thompson, George Richard; Sharma, Shobha; Bays, Derek J.; Pruitt, Rachel; Engelthaler, David M.; Bowers, Jolene; Driebe, Elizabeth M.; Davis, Michael; Libke, Robert; Cohen, Stuart H; Pappagianis, Demosthenes.

In: Chest, Vol. 143, No. 3, 03.2013, p. 776-781.

Research output: Contribution to journalArticle

Thompson, George Richard ; Sharma, Shobha ; Bays, Derek J. ; Pruitt, Rachel ; Engelthaler, David M. ; Bowers, Jolene ; Driebe, Elizabeth M. ; Davis, Michael ; Libke, Robert ; Cohen, Stuart H ; Pappagianis, Demosthenes. / Adenosine deaminase levels, serologic parameters, culture results, and polymerase chain reaction testing in pleural fluid. In: Chest. 2013 ; Vol. 143, No. 3. pp. 776-781.
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abstract = "Background: In a patient with positive serum serology for coccidioidomycosis, the differential diagnosis of concurrent pleural effusions can be challenging. We, therefore, sought to clarify the performance characteristics of biochemical, serologic, and nucleic-acid-based testing in an attempt to avoid invasive procedures. The utility of adenosine deaminase (ADA), coccidioidal serology, and polymerase chain reaction (PCR) in the evaluation of pleuropulmonary coccidioidomycosis has not been previously reported. Methods: Forty consecutive patients evaluated for pleuropulmonary coccidioidomycosis were included. Demographic data, pleural fluid values, culture results, and clinical diagnoses were obtained from patient chart review. ADA testing was performed by ARUP Laboratories, coccidioidal serologic testing was performed by the University of California-Davis coccidioidomycosis serology laboratory, and PCR testing was performed by the Translational Genomics Research Institute using a previously published methodology. Results: Fifteen patients were diagnosed with pleuropulmonary coccidioidomycosis by European Organization for the Research and Treatment of Cancer/Mycoses Study Group criteria. Pleural fluid ADA concentrations were <40 IU/L in all patients (range, <1.0-28.6 IU/L; median, 4.7). The sensitivity and specificity of coccidioidal serologic testing was 100{\%} in this study. The specificity of PCR testing was high (100{\%}), although the overall sensitivity remained low, and was comparable to the experience of others in the clinical use of PCR for coccidioidal diagnostics. Conclusion: Contrary to prior speculation, ADA levels in pleuropulmonary coccidioidomycosis were not elevated in this study. The sensitivity and specificity of coccidioidal serologic testing in nonserum samples remained high, but the clinical usefulness of PCR testing in pleural fluid was disappointing and was comparable to pleural fluid culture.",
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AU - Sharma, Shobha

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AU - Engelthaler, David M.

AU - Bowers, Jolene

AU - Driebe, Elizabeth M.

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AU - Libke, Robert

AU - Cohen, Stuart H

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