Adeno-associated virus 9-mediated airway expression of antibody protects old and immunodeficient mice against influenza virus

Virginie S. Adam, Marco Crosariol, Sachin Kumar, Moyar Q. Ge, Sarah E. Czack, Soumitra Roy, Angela Franciska Haczku, Anna Tretiakova, James M. Wilson, Maria P. Limberis

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Influenza causes serious and sometimes fatal disease in individuals at risk due to advanced age or immunodeficiencies. Despite progress in the development of seasonal influenza vaccines, vaccine efficacy in elderly and immunocompromised individuals remains low. We recently developed a passive immunization strategy using an adeno-associated virus (AAV) vector to deliver a neutralizing anti-influenza antibody at the site of infection, the nasal airways. Here we show that young, old, and immunodeficient (severe combined immunodeficient [SCID]) mice that were treated intranasally with AAV9 vector expressing a modified version of the broadly neutralizing anti-influenza antibody FI6 were protected and exhibited no signs of disease following an intranasal challenge with the mouse-adapted H1N1 influenza strain A/Puerto Rico/8/1934(H1N1) (PR8) (Mt. Sinai strain). Nonvaccinated mice succumbed to the PR8 challenge due to severe weight loss. We propose that airway-directed AAV9 passive immunization against airborne infectious agents may be beneficial in elderly and immunocompromised patients, for whom there still exists an unmet need for effective vaccination against influenza.

Original languageEnglish (US)
Pages (from-to)1528-1533
Number of pages6
JournalClinical and Vaccine Immunology
Volume21
Issue number11
DOIs
StatePublished - Nov 1 2014
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry
  • Microbiology (medical)

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    Adam, V. S., Crosariol, M., Kumar, S., Ge, M. Q., Czack, S. E., Roy, S., Haczku, A. F., Tretiakova, A., Wilson, J. M., & Limberis, M. P. (2014). Adeno-associated virus 9-mediated airway expression of antibody protects old and immunodeficient mice against influenza virus. Clinical and Vaccine Immunology, 21(11), 1528-1533. https://doi.org/10.1128/CVI.00572-14