Adaptive optics-optical coherence tomography: Optimizing visualization of microscopic retinal structures in three dimensions

Robert Zawadzki, Stacey S. Choi, Steven M. Jones, Scot S. Oliver, John S Werner

Research output: Contribution to journalArticle

121 Scopus citations

Abstract

Adaptive optics-optical coherence tomography (AO-OCT) permits improved imaging of microscopic retinal structures by combining the high lateral resolution of AO with the high axial resolution of OCT, resulting in the narrowest three-dimensional (3D) point-spread function (PSF) of all in vivo retinal imaging techniques. Owing to the high volumetric resolution of AO-OCT systems, it is now possible, for the first time, to acquire images of 3D cellular structures in the living retina. Thus, with AO-OCT, those retinal structures that are not visible with AO or OCT alone (e.g., bundles of retinal nerve fiber layers, 3D mosaic of photoreceptors, 3D structure of microvasculature, and detailed structure of retinal disruptions) can be visualized. Our current AO-OCT instrumentation uses spectrometer-based Fourier-domain OCT technology and two-deformable-mirror-based AO wavefront correction. We describe image processing methods that help to remove motion artifacts observed in volumetric data, followed by innovative data visualization techniques [including two-dimensional (2D) and 3D representations]. Finally, examples of microscopic retinal structures that are acquired with the University of California Davis AO-OCT system are presented.

Original languageEnglish (US)
Pages (from-to)1373-1383
Number of pages11
JournalJournal of the Optical Society of America A: Optics and Image Science, and Vision
Volume24
Issue number5
DOIs
StatePublished - 2007

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Computer Vision and Pattern Recognition

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