Adaptive mutation: General mutagenesis is not a programmed response to stress but results from rare coamplification of dinB with lac

E. Susan Slechta, Kim L. Bunny, Elisabeth Kugelberg, Eric Kofoid, Dan I. Andersson, John R. Roth

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

In a particular genetic system, selection stimulates reversion of a lac mutation and causes genome-wide mutagenesis (adaptive mutation). Selection allows rare plated cells with a duplication of the leaky lac allele to initiate clones within which further lac amplification improves growth rate. Growth and amplification add mutational targets to each clone and thereby increase the likelihood of reversion. We suggest that general mutagenesis occurs only in clones whose lac amplification includes the nearby dinB+ gene (for error-prone DNA polymerase IV). Thus mutagenesis is not a programmed response to stress but a side effect of amplification in a few clones; it is not central to the effect of selection on reversion.

Original languageEnglish (US)
Pages (from-to)12847-12852
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number22
DOIs
StatePublished - Oct 28 2003

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Mutagenesis
Clone Cells
Mutation
DNA Polymerase beta
Growth
Alleles
Genome
Genes

ASJC Scopus subject areas

  • Genetics
  • General

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Adaptive mutation : General mutagenesis is not a programmed response to stress but results from rare coamplification of dinB with lac. / Slechta, E. Susan; Bunny, Kim L.; Kugelberg, Elisabeth; Kofoid, Eric; Andersson, Dan I.; Roth, John R.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, No. 22, 28.10.2003, p. 12847-12852.

Research output: Contribution to journalArticle

Slechta, E. Susan ; Bunny, Kim L. ; Kugelberg, Elisabeth ; Kofoid, Eric ; Andersson, Dan I. ; Roth, John R. / Adaptive mutation : General mutagenesis is not a programmed response to stress but results from rare coamplification of dinB with lac. In: Proceedings of the National Academy of Sciences of the United States of America. 2003 ; Vol. 100, No. 22. pp. 12847-12852.
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