Adaptations of skeletal muscle to exercise: Rapid increase in the transcriptional coactivator PGC-1

Keith Baar, Adam R. Wende, Terry E. Jones, Matthew Marison, Lorraine A. Nolte, May Chen, Daniel P. Kelly, John O. Holloszy

Research output: Contribution to journalArticle

665 Citations (Scopus)

Abstract

Endurance exercise induces increases in mitochondria and the GLUT4 isoform of the glucose transporter in muscle. Although little is known about the mechanisms underlying these adaptations, new information has accumulated regarding how mitochondrial biogenesis and GLUT4 expression are regulated. This includes the findings that the transcriptional coactivator PGC-1 promotes mitochondrial biogenesis and that NRF-1 and NRF-2 act as transcriptional activators of genes encoding mitochondrial enzymes. We tested the hypothesis that increases in PGC-1, NRF-1, and NRF-2 are involved in the initial adaptive response of muscle to exercise. Five daily bouts of swimming induced increases in mitochondrial enzymes and GLUT4 in skeletal muscle in rats. One exercise bout resulted in ∼ twofold increases in full-length muscle PGC-1 mRNA and PGC-1 protein, which were evident 18 h after exercise. A smaller form of PGC-1 increased after exercise. The exercise induced increases in muscle NRF-1 and NRF-2 that were evident 12 to 18 h after one exercise bout. These findings suggest that increases in PGC-1, NRF-1, NRFd, and NRF-2 represent key regulatory components of the stimulation of mitochondrial biogenesis by exercise and that PGC-1 mediates the coordinated increases in GLUT4 and mitochondria.

Original languageEnglish (US)
Pages (from-to)1879-1886
Number of pages8
JournalFASEB Journal
Volume16
Issue number14
DOIs
StatePublished - Dec 2002
Externally publishedYes

Fingerprint

Muscle
skeletal muscle
Skeletal Muscle
exercise
Organelle Biogenesis
Muscles
Mitochondria
muscles
Mitochondrial Genes
Facilitative Glucose Transport Proteins
Enzymes
Gene encoding
Protein Isoforms
mitochondria
Rats
Durability
glucose transporters
Messenger RNA
enzymes
Proteins

Keywords

  • GLUT4
  • Mitochondrial biogenesis
  • NRF-1
  • NRF-2

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Baar, K., Wende, A. R., Jones, T. E., Marison, M., Nolte, L. A., Chen, M., ... Holloszy, J. O. (2002). Adaptations of skeletal muscle to exercise: Rapid increase in the transcriptional coactivator PGC-1. FASEB Journal, 16(14), 1879-1886. https://doi.org/10.1096/fj.02-0367com

Adaptations of skeletal muscle to exercise : Rapid increase in the transcriptional coactivator PGC-1. / Baar, Keith; Wende, Adam R.; Jones, Terry E.; Marison, Matthew; Nolte, Lorraine A.; Chen, May; Kelly, Daniel P.; Holloszy, John O.

In: FASEB Journal, Vol. 16, No. 14, 12.2002, p. 1879-1886.

Research output: Contribution to journalArticle

Baar, K, Wende, AR, Jones, TE, Marison, M, Nolte, LA, Chen, M, Kelly, DP & Holloszy, JO 2002, 'Adaptations of skeletal muscle to exercise: Rapid increase in the transcriptional coactivator PGC-1', FASEB Journal, vol. 16, no. 14, pp. 1879-1886. https://doi.org/10.1096/fj.02-0367com
Baar, Keith ; Wende, Adam R. ; Jones, Terry E. ; Marison, Matthew ; Nolte, Lorraine A. ; Chen, May ; Kelly, Daniel P. ; Holloszy, John O. / Adaptations of skeletal muscle to exercise : Rapid increase in the transcriptional coactivator PGC-1. In: FASEB Journal. 2002 ; Vol. 16, No. 14. pp. 1879-1886.
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