Adaptation of an Automated Assay for Determination of β‐Hydroxybutyrate in Dogs Using a Random Access Analyzer

Mary M Christopher, Jacqueline L. Periera, Robin L. Brigmon

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

An automated method for measuring β‐hydroxybutyrate was adapted to the Ciba‐Corning 550 Express™ random access analyzer. The assay was based on a kinetic reaction utilizing hydroxybutyrate‐dehydrogenase. β‐hydroxybutyrate concentration (mmol/L) was calculated ratiometrically using a 1.0 mmol/L standard. Canine serum, plasma, and urine were used without prior deproteinization and only a 30‐μl sample was required. The method demonstrated good linearity between 0 to 2 mmol/L of β‐hydroxybutyrate. Analytical recovery (accuracy) within these concentrations ranged from 85.8 to 113.3%. Both within‐run and day‐to‐day precision were determined, as was specificity of the assay in the presence of a variety of interfering substances. The automated assay was rapid and economical, with reagent stability maintained for at least 2 weeks at 4° C. This assay can readily be applied toward the assessment of ketoacidosis in dogs, and with further validation, other species.

Original languageEnglish (US)
Pages (from-to)3-8
Number of pages6
JournalVeterinary Clinical Pathology
Volume21
Issue number1
DOIs
StatePublished - 1992
Externally publishedYes

Fingerprint

Hydroxybutyrates
Dogs
dogs
assays
Ketosis
ketosis
Canidae
reaction kinetics
Urine
urine
Serum
methodology
sampling

Keywords

  • clinical chemistry
  • diabetes mellitus
  • ketone bodies
  • ketones

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Adaptation of an Automated Assay for Determination of β‐Hydroxybutyrate in Dogs Using a Random Access Analyzer. / Christopher, Mary M; Periera, Jacqueline L.; Brigmon, Robin L.

In: Veterinary Clinical Pathology, Vol. 21, No. 1, 1992, p. 3-8.

Research output: Contribution to journalArticle

@article{e08e0b66bf0144439c28641d5de10804,
title = "Adaptation of an Automated Assay for Determination of β‐Hydroxybutyrate in Dogs Using a Random Access Analyzer",
abstract = "An automated method for measuring β‐hydroxybutyrate was adapted to the Ciba‐Corning 550 Express™ random access analyzer. The assay was based on a kinetic reaction utilizing hydroxybutyrate‐dehydrogenase. β‐hydroxybutyrate concentration (mmol/L) was calculated ratiometrically using a 1.0 mmol/L standard. Canine serum, plasma, and urine were used without prior deproteinization and only a 30‐μl sample was required. The method demonstrated good linearity between 0 to 2 mmol/L of β‐hydroxybutyrate. Analytical recovery (accuracy) within these concentrations ranged from 85.8 to 113.3{\%}. Both within‐run and day‐to‐day precision were determined, as was specificity of the assay in the presence of a variety of interfering substances. The automated assay was rapid and economical, with reagent stability maintained for at least 2 weeks at 4° C. This assay can readily be applied toward the assessment of ketoacidosis in dogs, and with further validation, other species.",
keywords = "clinical chemistry, diabetes mellitus, ketone bodies, ketones",
author = "Christopher, {Mary M} and Periera, {Jacqueline L.} and Brigmon, {Robin L.}",
year = "1992",
doi = "10.1111/j.1939-165X.1992.tb00573.x",
language = "English (US)",
volume = "21",
pages = "3--8",
journal = "Veterinary Clinical Pathology",
issn = "0275-6382",
publisher = "American Society for Veterinary Clinical Pathology",
number = "1",

}

TY - JOUR

T1 - Adaptation of an Automated Assay for Determination of β‐Hydroxybutyrate in Dogs Using a Random Access Analyzer

AU - Christopher, Mary M

AU - Periera, Jacqueline L.

AU - Brigmon, Robin L.

PY - 1992

Y1 - 1992

N2 - An automated method for measuring β‐hydroxybutyrate was adapted to the Ciba‐Corning 550 Express™ random access analyzer. The assay was based on a kinetic reaction utilizing hydroxybutyrate‐dehydrogenase. β‐hydroxybutyrate concentration (mmol/L) was calculated ratiometrically using a 1.0 mmol/L standard. Canine serum, plasma, and urine were used without prior deproteinization and only a 30‐μl sample was required. The method demonstrated good linearity between 0 to 2 mmol/L of β‐hydroxybutyrate. Analytical recovery (accuracy) within these concentrations ranged from 85.8 to 113.3%. Both within‐run and day‐to‐day precision were determined, as was specificity of the assay in the presence of a variety of interfering substances. The automated assay was rapid and economical, with reagent stability maintained for at least 2 weeks at 4° C. This assay can readily be applied toward the assessment of ketoacidosis in dogs, and with further validation, other species.

AB - An automated method for measuring β‐hydroxybutyrate was adapted to the Ciba‐Corning 550 Express™ random access analyzer. The assay was based on a kinetic reaction utilizing hydroxybutyrate‐dehydrogenase. β‐hydroxybutyrate concentration (mmol/L) was calculated ratiometrically using a 1.0 mmol/L standard. Canine serum, plasma, and urine were used without prior deproteinization and only a 30‐μl sample was required. The method demonstrated good linearity between 0 to 2 mmol/L of β‐hydroxybutyrate. Analytical recovery (accuracy) within these concentrations ranged from 85.8 to 113.3%. Both within‐run and day‐to‐day precision were determined, as was specificity of the assay in the presence of a variety of interfering substances. The automated assay was rapid and economical, with reagent stability maintained for at least 2 weeks at 4° C. This assay can readily be applied toward the assessment of ketoacidosis in dogs, and with further validation, other species.

KW - clinical chemistry

KW - diabetes mellitus

KW - ketone bodies

KW - ketones

UR - http://www.scopus.com/inward/record.url?scp=84987205381&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84987205381&partnerID=8YFLogxK

U2 - 10.1111/j.1939-165X.1992.tb00573.x

DO - 10.1111/j.1939-165X.1992.tb00573.x

M3 - Article

AN - SCOPUS:84987205381

VL - 21

SP - 3

EP - 8

JO - Veterinary Clinical Pathology

JF - Veterinary Clinical Pathology

SN - 0275-6382

IS - 1

ER -