Adaptation of an Automated Assay for Determination of β‐Hydroxybutyrate in Dogs Using a Random Access Analyzer

Mary M Christopher, Jacqueline L. Periera, Robin L. Brigmon

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

An automated method for measuring β‐hydroxybutyrate was adapted to the Ciba‐Corning 550 Express™ random access analyzer. The assay was based on a kinetic reaction utilizing hydroxybutyrate‐dehydrogenase. β‐hydroxybutyrate concentration (mmol/L) was calculated ratiometrically using a 1.0 mmol/L standard. Canine serum, plasma, and urine were used without prior deproteinization and only a 30‐μl sample was required. The method demonstrated good linearity between 0 to 2 mmol/L of β‐hydroxybutyrate. Analytical recovery (accuracy) within these concentrations ranged from 85.8 to 113.3%. Both within‐run and day‐to‐day precision were determined, as was specificity of the assay in the presence of a variety of interfering substances. The automated assay was rapid and economical, with reagent stability maintained for at least 2 weeks at 4° C. This assay can readily be applied toward the assessment of ketoacidosis in dogs, and with further validation, other species.

Original languageEnglish (US)
Pages (from-to)3-8
Number of pages6
JournalVeterinary Clinical Pathology
Volume21
Issue number1
DOIs
StatePublished - 1992
Externally publishedYes

Keywords

  • clinical chemistry
  • diabetes mellitus
  • ketone bodies
  • ketones

ASJC Scopus subject areas

  • veterinary(all)

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