Adaptation of a mallard H5N2 low pathogenicity influenza virus in chickens with prior history of infection with infectious bursal disease virus

Gloria Ramirez-Nieto, H L Shivaprasad, Chul Hong Kim, Hyun Soon Lillehoj, Haichen Song, Ivan Gomez Osorio, Daniel R. Perez

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

The influenza A/Mallard/Pennsylvania/10218/1984 (H5N2) virus is unable to replicate in 3-wk-old immunocompetent specific-pathogen-free chickens when a dose of 5 × 106 50 egg infectious dose/ml is used. In contrast, this mallard virus shows limited replication in 3-wk-old chickens that had been previously infected at 2 days of age with, and recovered from, the immunosuppressive agent infectious bursal disease virus (IBDV; herein referred to as IBDV chickens). This limited replication in IBDV chickens allowed for the serial passage of the mallard influenza virus in chickens. After 22 passages (P22) in IBDV chickens, the resulting chicken-adapted influenza virus replicated in both immunocompetent and IBDV chickens more efficiently than the mallard influenza virus. Analysis of the outcomes of infection and the lesions caused by the two viruses at the microscopic level in a time-point study showed that the P22 virus is more virulent than the parental mallard virus in both immunocompetent and IBDV chickens. Our studies provide evidence that a previous history of IBDV infection in chickens may render them more susceptible to avian influenza virus (AIV) infections, allowing for the potential introduction of AIVs in an otherwise resistant population.

Original languageEnglish (US)
Pages (from-to)513-521
Number of pages9
JournalAvian Diseases
Volume54
Issue numberSUPPL. 1
DOIs
StatePublished - Mar 2010

Keywords

  • Adaptation
  • Avian influenza
  • Infectious bursal disease virus
  • Pathogenesis

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Food Animals
  • Immunology and Microbiology(all)

Fingerprint Dive into the research topics of 'Adaptation of a mallard H5N2 low pathogenicity influenza virus in chickens with prior history of infection with infectious bursal disease virus'. Together they form a unique fingerprint.

Cite this