ADAM22, a Kv1 channel-interacting protein, recruits membrane-associated guanylate kinases to juxtaparanodes of myelinated axons

Yasuhiro Ogawa, Juan Oses-Prieto, Moon Young Kim, Ido Horresh, Elior Peles, Alma L. Burlingame, James Trimmer, Dies Meijer, Matthew N. Rasband

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Abstract

Clustered Kv1 K+ channels regulate neuronal excitability at juxtaparanodes of myelinated axons, axon initial segments, and cerebellar basket cell terminals (BCTs). These channels are part of a larger protein complex that includes cell adhesion molecules and scaffolding proteins. To identify proteins that regulate assembly, clustering, and/or maintenance of axonal Kv1 channel protein complexes, we immunoprecipitated Kv1.2 αsubunits, and then used mass spectrometry to identify interacting proteins.We found that a disintegrin and metalloproteinase 22 (ADAM22) is a component of the Kv1 channel complex and that ADAM22 coimmunoprecipitates Kv1.2 and the membrane-associated guanylate kinases (MAGUKs) PSD-93 and PSD-95. When coexpressed with MAGUKs in heterologous cells, ADAM22 and Kv1 channels are recruited into membrane surface clusters. However, coexpression of Kv1.2 with ADAM22 and MAGUKs does not alter channel properties. Among all the known Kv1 channel-interacting proteins, only ADAM22 is found at every site where Kv1 channels are clustered. Analysis of Caspr-null mice showed that, like other previously described juxtaparanodal proteins, disruption of the paranodal junction resulted in redistribution of ADAM22 into paranodal zones. Analysis of Caspr2-, PSD-93-, PSD-95-, and double PSD-93/PSD-95-null mice showed ADAM22 clustering at BCTs requires PSD-95, but ADAM22 clustering at juxtaparanodes requires neither PSD-93 nor PSD-95. In direct contrast, analysis of ADAM22-null mice demonstrated juxtaparanodal clustering of PSD-93 and PSD-95 requires ADAM22, whereas Kv1.2 and Caspr2 clustering is normal in ADAM22-null mice. Thus, ADAM22 is an axonal component of the Kv1 K+ channel complex that recruits MAGUKs to juxtaparanodes.

Original languageEnglish (US)
Pages (from-to)1038-1048
Number of pages11
JournalJournal of Neuroscience
Volume30
Issue number3
DOIs
StatePublished - Jan 20 2010

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Guanylate Kinases
Disintegrins
Metalloproteases
Axons
Proteins
Cluster Analysis
Cell Adhesion Molecules

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)

Cite this

Ogawa, Y., Oses-Prieto, J., Kim, M. Y., Horresh, I., Peles, E., Burlingame, A. L., ... Rasband, M. N. (2010). ADAM22, a Kv1 channel-interacting protein, recruits membrane-associated guanylate kinases to juxtaparanodes of myelinated axons. Journal of Neuroscience, 30(3), 1038-1048. https://doi.org/10.1523/JNEUROSCI.4661-09.2010

ADAM22, a Kv1 channel-interacting protein, recruits membrane-associated guanylate kinases to juxtaparanodes of myelinated axons. / Ogawa, Yasuhiro; Oses-Prieto, Juan; Kim, Moon Young; Horresh, Ido; Peles, Elior; Burlingame, Alma L.; Trimmer, James; Meijer, Dies; Rasband, Matthew N.

In: Journal of Neuroscience, Vol. 30, No. 3, 20.01.2010, p. 1038-1048.

Research output: Contribution to journalArticle

Ogawa, Y, Oses-Prieto, J, Kim, MY, Horresh, I, Peles, E, Burlingame, AL, Trimmer, J, Meijer, D & Rasband, MN 2010, 'ADAM22, a Kv1 channel-interacting protein, recruits membrane-associated guanylate kinases to juxtaparanodes of myelinated axons', Journal of Neuroscience, vol. 30, no. 3, pp. 1038-1048. https://doi.org/10.1523/JNEUROSCI.4661-09.2010
Ogawa, Yasuhiro ; Oses-Prieto, Juan ; Kim, Moon Young ; Horresh, Ido ; Peles, Elior ; Burlingame, Alma L. ; Trimmer, James ; Meijer, Dies ; Rasband, Matthew N. / ADAM22, a Kv1 channel-interacting protein, recruits membrane-associated guanylate kinases to juxtaparanodes of myelinated axons. In: Journal of Neuroscience. 2010 ; Vol. 30, No. 3. pp. 1038-1048.
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abstract = "Clustered Kv1 K+ channels regulate neuronal excitability at juxtaparanodes of myelinated axons, axon initial segments, and cerebellar basket cell terminals (BCTs). These channels are part of a larger protein complex that includes cell adhesion molecules and scaffolding proteins. To identify proteins that regulate assembly, clustering, and/or maintenance of axonal Kv1 channel protein complexes, we immunoprecipitated Kv1.2 αsubunits, and then used mass spectrometry to identify interacting proteins.We found that a disintegrin and metalloproteinase 22 (ADAM22) is a component of the Kv1 channel complex and that ADAM22 coimmunoprecipitates Kv1.2 and the membrane-associated guanylate kinases (MAGUKs) PSD-93 and PSD-95. When coexpressed with MAGUKs in heterologous cells, ADAM22 and Kv1 channels are recruited into membrane surface clusters. However, coexpression of Kv1.2 with ADAM22 and MAGUKs does not alter channel properties. Among all the known Kv1 channel-interacting proteins, only ADAM22 is found at every site where Kv1 channels are clustered. Analysis of Caspr-null mice showed that, like other previously described juxtaparanodal proteins, disruption of the paranodal junction resulted in redistribution of ADAM22 into paranodal zones. Analysis of Caspr2-, PSD-93-, PSD-95-, and double PSD-93/PSD-95-null mice showed ADAM22 clustering at BCTs requires PSD-95, but ADAM22 clustering at juxtaparanodes requires neither PSD-93 nor PSD-95. In direct contrast, analysis of ADAM22-null mice demonstrated juxtaparanodal clustering of PSD-93 and PSD-95 requires ADAM22, whereas Kv1.2 and Caspr2 clustering is normal in ADAM22-null mice. Thus, ADAM22 is an axonal component of the Kv1 K+ channel complex that recruits MAGUKs to juxtaparanodes.",
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AU - Horresh, Ido

AU - Peles, Elior

AU - Burlingame, Alma L.

AU - Trimmer, James

AU - Meijer, Dies

AU - Rasband, Matthew N.

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