ADAM12-mediated focal adhesion formation is differently regulated by β1 and β3 integrins

Charles Kumar Thodeti, Camilla Fröhlich, Christian Kamp Nielsen, Yoshikazu Takada, Reinhard Fässler, Reidar Albrechtsen, Ulla M. Wewer

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

ADAM12, a disintegrin and metalloprotease, has been demonstrated to be upregulated in human malignant tumors and to accelerate the malignant phenotype in a mouse model for breast cancer. ADAM12 is a substrate for β1 integrins and may affect tumor and stromal cell behavior through its binding to β1 integrins. Here, we report that cells deficient in β1 integrin or overexpressing β3 integrin can bind to recombinant full-length human ADAM12 via β3 integrin. Furthermore, cell binding to ADAM12 via β3 integrin results in the formation of focal adhesions, which are not formed upon β1 integrin-mediated cell attachment. We also show that RhoA is involved in β3 integrin-mediated focal adhesion formation.

Original languageEnglish (US)
Pages (from-to)5589-5595
Number of pages7
JournalFEBS Letters
Volume579
Issue number25
DOIs
StatePublished - Oct 24 2005

Fingerprint

Focal Adhesions
Integrins
Adhesion
Tumors
Disintegrins
Metalloproteases
Stromal Cells
Neoplasms
Breast Neoplasms
Phenotype
Substrates

Keywords

  • ADAM12 integrin
  • Cancer
  • Cell adhesion
  • Focal adhesion
  • RhoA

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Thodeti, C. K., Fröhlich, C., Nielsen, C. K., Takada, Y., Fässler, R., Albrechtsen, R., & Wewer, U. M. (2005). ADAM12-mediated focal adhesion formation is differently regulated by β1 and β3 integrins. FEBS Letters, 579(25), 5589-5595. https://doi.org/10.1016/j.febslet.2005.09.024

ADAM12-mediated focal adhesion formation is differently regulated by β1 and β3 integrins. / Thodeti, Charles Kumar; Fröhlich, Camilla; Nielsen, Christian Kamp; Takada, Yoshikazu; Fässler, Reinhard; Albrechtsen, Reidar; Wewer, Ulla M.

In: FEBS Letters, Vol. 579, No. 25, 24.10.2005, p. 5589-5595.

Research output: Contribution to journalArticle

Thodeti, CK, Fröhlich, C, Nielsen, CK, Takada, Y, Fässler, R, Albrechtsen, R & Wewer, UM 2005, 'ADAM12-mediated focal adhesion formation is differently regulated by β1 and β3 integrins', FEBS Letters, vol. 579, no. 25, pp. 5589-5595. https://doi.org/10.1016/j.febslet.2005.09.024
Thodeti CK, Fröhlich C, Nielsen CK, Takada Y, Fässler R, Albrechtsen R et al. ADAM12-mediated focal adhesion formation is differently regulated by β1 and β3 integrins. FEBS Letters. 2005 Oct 24;579(25):5589-5595. https://doi.org/10.1016/j.febslet.2005.09.024
Thodeti, Charles Kumar ; Fröhlich, Camilla ; Nielsen, Christian Kamp ; Takada, Yoshikazu ; Fässler, Reinhard ; Albrechtsen, Reidar ; Wewer, Ulla M. / ADAM12-mediated focal adhesion formation is differently regulated by β1 and β3 integrins. In: FEBS Letters. 2005 ; Vol. 579, No. 25. pp. 5589-5595.
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