Acute tobacco smoke exposure promotes mitochondrial permeability transition in rat heart

Matt Eaton, Hemamalini Gursahani, Yehuda Arieli, Kent E Pinkerton, Saul Schaefer

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Chronic exposure to tobacco smoke is known to impair mitochondrial function. However, the effect of acute tobacco smoke exposure (ATSE) in vivo, as might occur in social settings, on mitochondrial function and calcium handling of cardiac cells has not been examined. It was hypothesized that ATSE might adversely modify mitochondrial function as reflected in mitochondrial energetics, membrane potential, and calcium transport. Mitochondria were isolated from the hearts of adult rats either exposed to 6 h of environmental tobacco smoke (∼60 mg/mm 3 tobacco smoke particles) or sham exposure. To model a calcium stress similar to ischemia/reperfusion, mitochondria were exposed to a Ca 2+ bolus with measurement of membrane potential, energetics, Ca 2+ uptake and release, and redox state. ATSE mitochondria were characterized by significantly higher ADP-stimulated ATP production and a more reduced redox state (NADH ratio) under basal conditions without observed changes in resting Ψ m. Exposure of ATSE mitochondria to Ca 2+ stress resulted in significantly more rapid depolarization of Ψ m. The initial rate of Ca 2+ uptake was not altered in ATSE mitochondria, but CsA-sensitive Ca 2+ release was significantly increased. ATSE does not significantly alter resting mitochondrial function. However, ATSE modifies the response of cardiac mitochondria to calcium stress, resulting in a more rapid depolarization and subsequent release of Ca 2+ via the mitochondrial permeability transition (MPT). Copyright

Original languageEnglish (US)
Pages (from-to)1497-1510
Number of pages14
JournalJournal of Toxicology and Environmental Health - Part A: Current Issues
Volume69
Issue number15
DOIs
StatePublished - 2006

Fingerprint

Tobacco
Smoke
Rats
Permeability
Mitochondria
permeability
mitochondrion
Calcium
calcium
Depolarization
Oxidation-Reduction
energetics
exposure
tobacco smoke
membrane
Membranes
Administrative data processing
Mitochondrial Membrane Potential
Adenosinetriphosphate
NAD

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis
  • Environmental Science(all)
  • Environmental Chemistry
  • Public Health, Environmental and Occupational Health
  • Pollution

Cite this

Acute tobacco smoke exposure promotes mitochondrial permeability transition in rat heart. / Eaton, Matt; Gursahani, Hemamalini; Arieli, Yehuda; Pinkerton, Kent E; Schaefer, Saul.

In: Journal of Toxicology and Environmental Health - Part A: Current Issues, Vol. 69, No. 15, 2006, p. 1497-1510.

Research output: Contribution to journalArticle

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