TY - JOUR
T1 - Acute hyperoxia-induced transcriptional response in the mouse RPE/choroid
AU - Weigel, Andrea L.
AU - Ida, Hisashi
AU - Boylan, Sharon A.
AU - Hjelmeland, Leonard M
PY - 2003/9/1
Y1 - 2003/9/1
N2 - Oxidative stress has been studied in the retinal pigmented epithelium (RPE) in vitro but not in vivo. Our purpose, therefore, was to develop an in vivo model of acute oxidative stress in the C57BL/6J mouse. Mice were exposed to ≥ 98% oxygen for 0, 2, or 6 h, and amplified total RNA from the RPE/choroid was applied to microarrays examining about 2200 unique genes. Statistical analysis determined that 642 genes, out of a total of 1349 expressed, were significantly downregulated at only 2 h, only 6 h, or both 2 and 6 h, and a single gene, ubiquitin, was upregulated. These genes are involved in all aspects of cellular functions, and there are no major differences among the three groups. The effect of hyperoxia on the RPE/choroid in vivo appears to be very similar to oxidative stress studies performed with an RPE cell line in vitro. All 11 genes identified as being regulated by all three oxidants in our previous study, and were expressed by mouse, were also differentially regulated by hyperoxia. At least for the initial response to an oxidative challenge, the in vitro ARPE-19 cell line is a reasonable model for in vivo studies.
AB - Oxidative stress has been studied in the retinal pigmented epithelium (RPE) in vitro but not in vivo. Our purpose, therefore, was to develop an in vivo model of acute oxidative stress in the C57BL/6J mouse. Mice were exposed to ≥ 98% oxygen for 0, 2, or 6 h, and amplified total RNA from the RPE/choroid was applied to microarrays examining about 2200 unique genes. Statistical analysis determined that 642 genes, out of a total of 1349 expressed, were significantly downregulated at only 2 h, only 6 h, or both 2 and 6 h, and a single gene, ubiquitin, was upregulated. These genes are involved in all aspects of cellular functions, and there are no major differences among the three groups. The effect of hyperoxia on the RPE/choroid in vivo appears to be very similar to oxidative stress studies performed with an RPE cell line in vitro. All 11 genes identified as being regulated by all three oxidants in our previous study, and were expressed by mouse, were also differentially regulated by hyperoxia. At least for the initial response to an oxidative challenge, the in vitro ARPE-19 cell line is a reasonable model for in vivo studies.
KW - cDNA microarray
KW - Free radicals
KW - In vivo
KW - Oxidative stress
KW - RPE cells
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U2 - 10.1016/S0891-5849(03)00320-4
DO - 10.1016/S0891-5849(03)00320-4
M3 - Article
C2 - 12927596
AN - SCOPUS:0142043468
VL - 35
SP - 465
EP - 474
JO - Free Radical Biology and Medicine
JF - Free Radical Biology and Medicine
SN - 0891-5849
IS - 5
ER -