Acute hippocampal slice preparation and hippocampal slice cultures

Pamela J Lein, Christopher D. Barnhart, Isaac N Pessah

Research output: Chapter in Book/Report/Conference proceedingChapter

34 Scopus citations

Abstract

A major advantage of hippocampal slice preparations is that the cytoarchitecture and synaptic circuits of the hippocampus are largely retained. In neurotoxicology research, organotypic hippocampal slices have mostly been used as acute ex vivo preparations for investigating the effects of neurotoxic chemicals on synaptic function. More recently, hippocampal slice cultures, which can be maintained for several weeks to several months in vitro, have been employed to study how neurotoxic chemicals influence the structural and functional plasticity in hippocampal neurons. This chapter provides protocols for preparing hippocampal slices to be used acutely for electrophysiological measurements using glass microelectrodes or microelectrode arrays or to be cultured for morphometric assessments of individual neurons labeled using biolistics.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages115-134
Number of pages20
Volume758
DOIs
StatePublished - 2011

Publication series

NameMethods in Molecular Biology
Volume758
ISSN (Print)10643745

Keywords

  • Acute hippocampal slice
  • Biolistics
  • Electrophysiology
  • Hippocampal slice culture
  • Microelectrode arrays
  • Morphometry

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

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  • Cite this

    Lein, P. J., Barnhart, C. D., & Pessah, I. N. (2011). Acute hippocampal slice preparation and hippocampal slice cultures. In Methods in Molecular Biology (Vol. 758, pp. 115-134). (Methods in Molecular Biology; Vol. 758). https://doi.org/10.1007/978-1-61779-170-3_8