Acute effects of tumor necrosis factor on hydraulic conductivity of mammalian postcapillary venules

Ruby A. Skinner, Vicky L. Tucker, Fitz Roy E Curry

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background: Tumor necrosis factor (TNF) is a cytokine that has been implicated in the development of capillary leak in sepsis. Methods: We examined the direct effects of intraluminally applied TNF on microvessel hydraulic permeability (Lp) in the in situ mesentery of pentobarbital anesthetized female rats. Postcapillary venules were cannulated and perfused with Ringer's solution containing 0.5% albumin and washed erythrocytes. Transcapillary volume flow per unit surface area (Jv/S) was measured by using the modified Landis technique and Lp was calculated from the regression of Jv/S on pressures between 35 and 75 cm H2O. Results: Under control conditions the Lp (mean ± SE) was 1.06 ± 0.08 x 10-7 cm/(s · cm H2O) (n = 16). Lp was 0.87 ± 0.12 x 10-7 cm/(s · cm H2O) after a 20-minute perfusion with murine recombinant TNF at a concentration of 150 pg/mL (n = 5, p vs. control = 0.3). At a concentration of 10 ng/mL Lp was 1.15 ± 0.15 x 10-7 cm/(s · cm H2O) (n = 7, p vs. control = 0.6). In vessels perfused for 2 hours with TNF at 10 ng/mL, Lp was 0.96 ± 0.33 x 10-7 cm/(s · cm H2O) (n = 4, p = 0.66). At 100 ng/mL, Lp was 2.4 ± 0.40 x 10-7 cm/(s · cm H2O) (n = 7, p = 0.046). Conclusion: The acute intraluminal exposure to TNF, in the absence of other circulating factors, does not increase venular hydraulic permeability at concentrations of 150 pg/mL and 10 ng/mL. In vessels exposed at high or supraphysiologic concentrations (100 ng/mL), an acute twofold increase in Lp was demonstrated.

Original languageEnglish (US)
Pages (from-to)486-491
Number of pages6
JournalJournal of Trauma - Injury, Infection and Critical Care
Volume47
Issue number3
DOIs
StatePublished - Sep 1999

Fingerprint

Venules
Tumor Necrosis Factor-alpha
Permeability
Mesentery
Pentobarbital
Microvessels
Albumins
Sepsis
Perfusion
Erythrocytes
Cytokines
Pressure

ASJC Scopus subject areas

  • Surgery

Cite this

Acute effects of tumor necrosis factor on hydraulic conductivity of mammalian postcapillary venules. / Skinner, Ruby A.; Tucker, Vicky L.; Curry, Fitz Roy E.

In: Journal of Trauma - Injury, Infection and Critical Care, Vol. 47, No. 3, 09.1999, p. 486-491.

Research output: Contribution to journalArticle

@article{b5848b95c1dc4c8d859ce18def393d7c,
title = "Acute effects of tumor necrosis factor on hydraulic conductivity of mammalian postcapillary venules",
abstract = "Background: Tumor necrosis factor (TNF) is a cytokine that has been implicated in the development of capillary leak in sepsis. Methods: We examined the direct effects of intraluminally applied TNF on microvessel hydraulic permeability (Lp) in the in situ mesentery of pentobarbital anesthetized female rats. Postcapillary venules were cannulated and perfused with Ringer's solution containing 0.5{\%} albumin and washed erythrocytes. Transcapillary volume flow per unit surface area (Jv/S) was measured by using the modified Landis technique and Lp was calculated from the regression of Jv/S on pressures between 35 and 75 cm H2O. Results: Under control conditions the Lp (mean ± SE) was 1.06 ± 0.08 x 10-7 cm/(s · cm H2O) (n = 16). Lp was 0.87 ± 0.12 x 10-7 cm/(s · cm H2O) after a 20-minute perfusion with murine recombinant TNF at a concentration of 150 pg/mL (n = 5, p vs. control = 0.3). At a concentration of 10 ng/mL Lp was 1.15 ± 0.15 x 10-7 cm/(s · cm H2O) (n = 7, p vs. control = 0.6). In vessels perfused for 2 hours with TNF at 10 ng/mL, Lp was 0.96 ± 0.33 x 10-7 cm/(s · cm H2O) (n = 4, p = 0.66). At 100 ng/mL, Lp was 2.4 ± 0.40 x 10-7 cm/(s · cm H2O) (n = 7, p = 0.046). Conclusion: The acute intraluminal exposure to TNF, in the absence of other circulating factors, does not increase venular hydraulic permeability at concentrations of 150 pg/mL and 10 ng/mL. In vessels exposed at high or supraphysiologic concentrations (100 ng/mL), an acute twofold increase in Lp was demonstrated.",
author = "Skinner, {Ruby A.} and Tucker, {Vicky L.} and Curry, {Fitz Roy E}",
year = "1999",
month = "9",
doi = "10.1097/00005373-199909000-00009",
language = "English (US)",
volume = "47",
pages = "486--491",
journal = "Journal of Trauma and Acute Care Surgery",
issn = "2163-0755",
publisher = "Lippincott Williams and Wilkins",
number = "3",

}

TY - JOUR

T1 - Acute effects of tumor necrosis factor on hydraulic conductivity of mammalian postcapillary venules

AU - Skinner, Ruby A.

AU - Tucker, Vicky L.

AU - Curry, Fitz Roy E

PY - 1999/9

Y1 - 1999/9

N2 - Background: Tumor necrosis factor (TNF) is a cytokine that has been implicated in the development of capillary leak in sepsis. Methods: We examined the direct effects of intraluminally applied TNF on microvessel hydraulic permeability (Lp) in the in situ mesentery of pentobarbital anesthetized female rats. Postcapillary venules were cannulated and perfused with Ringer's solution containing 0.5% albumin and washed erythrocytes. Transcapillary volume flow per unit surface area (Jv/S) was measured by using the modified Landis technique and Lp was calculated from the regression of Jv/S on pressures between 35 and 75 cm H2O. Results: Under control conditions the Lp (mean ± SE) was 1.06 ± 0.08 x 10-7 cm/(s · cm H2O) (n = 16). Lp was 0.87 ± 0.12 x 10-7 cm/(s · cm H2O) after a 20-minute perfusion with murine recombinant TNF at a concentration of 150 pg/mL (n = 5, p vs. control = 0.3). At a concentration of 10 ng/mL Lp was 1.15 ± 0.15 x 10-7 cm/(s · cm H2O) (n = 7, p vs. control = 0.6). In vessels perfused for 2 hours with TNF at 10 ng/mL, Lp was 0.96 ± 0.33 x 10-7 cm/(s · cm H2O) (n = 4, p = 0.66). At 100 ng/mL, Lp was 2.4 ± 0.40 x 10-7 cm/(s · cm H2O) (n = 7, p = 0.046). Conclusion: The acute intraluminal exposure to TNF, in the absence of other circulating factors, does not increase venular hydraulic permeability at concentrations of 150 pg/mL and 10 ng/mL. In vessels exposed at high or supraphysiologic concentrations (100 ng/mL), an acute twofold increase in Lp was demonstrated.

AB - Background: Tumor necrosis factor (TNF) is a cytokine that has been implicated in the development of capillary leak in sepsis. Methods: We examined the direct effects of intraluminally applied TNF on microvessel hydraulic permeability (Lp) in the in situ mesentery of pentobarbital anesthetized female rats. Postcapillary venules were cannulated and perfused with Ringer's solution containing 0.5% albumin and washed erythrocytes. Transcapillary volume flow per unit surface area (Jv/S) was measured by using the modified Landis technique and Lp was calculated from the regression of Jv/S on pressures between 35 and 75 cm H2O. Results: Under control conditions the Lp (mean ± SE) was 1.06 ± 0.08 x 10-7 cm/(s · cm H2O) (n = 16). Lp was 0.87 ± 0.12 x 10-7 cm/(s · cm H2O) after a 20-minute perfusion with murine recombinant TNF at a concentration of 150 pg/mL (n = 5, p vs. control = 0.3). At a concentration of 10 ng/mL Lp was 1.15 ± 0.15 x 10-7 cm/(s · cm H2O) (n = 7, p vs. control = 0.6). In vessels perfused for 2 hours with TNF at 10 ng/mL, Lp was 0.96 ± 0.33 x 10-7 cm/(s · cm H2O) (n = 4, p = 0.66). At 100 ng/mL, Lp was 2.4 ± 0.40 x 10-7 cm/(s · cm H2O) (n = 7, p = 0.046). Conclusion: The acute intraluminal exposure to TNF, in the absence of other circulating factors, does not increase venular hydraulic permeability at concentrations of 150 pg/mL and 10 ng/mL. In vessels exposed at high or supraphysiologic concentrations (100 ng/mL), an acute twofold increase in Lp was demonstrated.

UR - http://www.scopus.com/inward/record.url?scp=0032864888&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032864888&partnerID=8YFLogxK

U2 - 10.1097/00005373-199909000-00009

DO - 10.1097/00005373-199909000-00009

M3 - Article

VL - 47

SP - 486

EP - 491

JO - Journal of Trauma and Acute Care Surgery

JF - Journal of Trauma and Acute Care Surgery

SN - 2163-0755

IS - 3

ER -