Activity-dependent glial swelling is impaired in aquaporin-4 knockout mice

Hiroki Kitaura, Mika Tsujita, Vincent J. Huber, Akiyoshi Kakita, Katsuei Shibuki, Kenji Sakimura, Ingrid Kwee, Tsutomu Nakada

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

We investigated the role of aquaporin-4 (AQP4), a water channel expressed in glial cells, in neural activity mediated morphological changes observed in brain slice preparation. Changes in flavoprotein fluorescence (FF) and infrared light scattering (LS) signals were measured before and after repetitive stimulation of layer VI in rostral somatosensory cortical slices taken from AQP4 knockout (KO) and wild-type (WT) mice. Changes in FF, which reflect neural aerobic activities, were comparable for the two groups in all cortical layers. However, changes in LS signals, which are indicative of cell swelling, were significantly decreased in layer I of AQP4 KO mice compared to that of WT mice. We conclude that AQP4 likely plays a significant role in neural activity-dependent glial swelling.

Original languageEnglish (US)
Pages (from-to)208-212
Number of pages5
JournalNeuroscience Research
Volume64
Issue number2
DOIs
StatePublished - Jun 2009

Fingerprint

Aquaporin 4
Knockout Mice
Neuroglia
Flavoproteins
Fluorescence
Light
Aquaporins
Brain

Keywords

  • Aquaporin-4
  • Astrocyte
  • Flavoprotein fluorescence
  • Knockout mouse
  • Light scattering
  • Somatosensory cortex

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Kitaura, H., Tsujita, M., Huber, V. J., Kakita, A., Shibuki, K., Sakimura, K., ... Nakada, T. (2009). Activity-dependent glial swelling is impaired in aquaporin-4 knockout mice. Neuroscience Research, 64(2), 208-212. https://doi.org/10.1016/j.neures.2009.03.002

Activity-dependent glial swelling is impaired in aquaporin-4 knockout mice. / Kitaura, Hiroki; Tsujita, Mika; Huber, Vincent J.; Kakita, Akiyoshi; Shibuki, Katsuei; Sakimura, Kenji; Kwee, Ingrid; Nakada, Tsutomu.

In: Neuroscience Research, Vol. 64, No. 2, 06.2009, p. 208-212.

Research output: Contribution to journalArticle

Kitaura, H, Tsujita, M, Huber, VJ, Kakita, A, Shibuki, K, Sakimura, K, Kwee, I & Nakada, T 2009, 'Activity-dependent glial swelling is impaired in aquaporin-4 knockout mice', Neuroscience Research, vol. 64, no. 2, pp. 208-212. https://doi.org/10.1016/j.neures.2009.03.002
Kitaura H, Tsujita M, Huber VJ, Kakita A, Shibuki K, Sakimura K et al. Activity-dependent glial swelling is impaired in aquaporin-4 knockout mice. Neuroscience Research. 2009 Jun;64(2):208-212. https://doi.org/10.1016/j.neures.2009.03.002
Kitaura, Hiroki ; Tsujita, Mika ; Huber, Vincent J. ; Kakita, Akiyoshi ; Shibuki, Katsuei ; Sakimura, Kenji ; Kwee, Ingrid ; Nakada, Tsutomu. / Activity-dependent glial swelling is impaired in aquaporin-4 knockout mice. In: Neuroscience Research. 2009 ; Vol. 64, No. 2. pp. 208-212.
@article{6fdb421eb3cd4fc582d8a51771e8fbf0,
title = "Activity-dependent glial swelling is impaired in aquaporin-4 knockout mice",
abstract = "We investigated the role of aquaporin-4 (AQP4), a water channel expressed in glial cells, in neural activity mediated morphological changes observed in brain slice preparation. Changes in flavoprotein fluorescence (FF) and infrared light scattering (LS) signals were measured before and after repetitive stimulation of layer VI in rostral somatosensory cortical slices taken from AQP4 knockout (KO) and wild-type (WT) mice. Changes in FF, which reflect neural aerobic activities, were comparable for the two groups in all cortical layers. However, changes in LS signals, which are indicative of cell swelling, were significantly decreased in layer I of AQP4 KO mice compared to that of WT mice. We conclude that AQP4 likely plays a significant role in neural activity-dependent glial swelling.",
keywords = "Aquaporin-4, Astrocyte, Flavoprotein fluorescence, Knockout mouse, Light scattering, Somatosensory cortex",
author = "Hiroki Kitaura and Mika Tsujita and Huber, {Vincent J.} and Akiyoshi Kakita and Katsuei Shibuki and Kenji Sakimura and Ingrid Kwee and Tsutomu Nakada",
year = "2009",
month = "6",
doi = "10.1016/j.neures.2009.03.002",
language = "English (US)",
volume = "64",
pages = "208--212",
journal = "Neuroscience Research",
issn = "0168-0102",
publisher = "Elsevier Ireland Ltd",
number = "2",

}

TY - JOUR

T1 - Activity-dependent glial swelling is impaired in aquaporin-4 knockout mice

AU - Kitaura, Hiroki

AU - Tsujita, Mika

AU - Huber, Vincent J.

AU - Kakita, Akiyoshi

AU - Shibuki, Katsuei

AU - Sakimura, Kenji

AU - Kwee, Ingrid

AU - Nakada, Tsutomu

PY - 2009/6

Y1 - 2009/6

N2 - We investigated the role of aquaporin-4 (AQP4), a water channel expressed in glial cells, in neural activity mediated morphological changes observed in brain slice preparation. Changes in flavoprotein fluorescence (FF) and infrared light scattering (LS) signals were measured before and after repetitive stimulation of layer VI in rostral somatosensory cortical slices taken from AQP4 knockout (KO) and wild-type (WT) mice. Changes in FF, which reflect neural aerobic activities, were comparable for the two groups in all cortical layers. However, changes in LS signals, which are indicative of cell swelling, were significantly decreased in layer I of AQP4 KO mice compared to that of WT mice. We conclude that AQP4 likely plays a significant role in neural activity-dependent glial swelling.

AB - We investigated the role of aquaporin-4 (AQP4), a water channel expressed in glial cells, in neural activity mediated morphological changes observed in brain slice preparation. Changes in flavoprotein fluorescence (FF) and infrared light scattering (LS) signals were measured before and after repetitive stimulation of layer VI in rostral somatosensory cortical slices taken from AQP4 knockout (KO) and wild-type (WT) mice. Changes in FF, which reflect neural aerobic activities, were comparable for the two groups in all cortical layers. However, changes in LS signals, which are indicative of cell swelling, were significantly decreased in layer I of AQP4 KO mice compared to that of WT mice. We conclude that AQP4 likely plays a significant role in neural activity-dependent glial swelling.

KW - Aquaporin-4

KW - Astrocyte

KW - Flavoprotein fluorescence

KW - Knockout mouse

KW - Light scattering

KW - Somatosensory cortex

UR - http://www.scopus.com/inward/record.url?scp=67349268190&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67349268190&partnerID=8YFLogxK

U2 - 10.1016/j.neures.2009.03.002

DO - 10.1016/j.neures.2009.03.002

M3 - Article

C2 - 19428702

AN - SCOPUS:67349268190

VL - 64

SP - 208

EP - 212

JO - Neuroscience Research

JF - Neuroscience Research

SN - 0168-0102

IS - 2

ER -