Activity and distribution of lysosomal enzymes during collagenous matrix-induced cartilage, bone, and bone marrow development

N. C. Rath, A. R. Hand, A Hari Reddi

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The appearance of the lysosomal enzymes acid phosphatase, arylsulfatase, and β-glucuronidase was studied during endochondral bone and bone marrow formation induced by implantation of demineralized bone matrix. The activities of acid phosphatase and β-glucuronidase gradually increased from the stage of mesenchymal cell proliferation on Day 3 onward to reach a peak on Day 13, during maximal bone remodeling. However, arysulfatase activity exhibited a sharp increase on Day 9, associated with the onset of cartilage hypertrophy and chondrolysis. The peak of arylsulfatase activity was also attained on Day 13. The activities of all three enzymes declined on Day 15 but acid phosphatase again exhibited an increase during hematopoietic bone marrow differentiation on Days 19-21. Histochemical and ultrastructural studies revealed intense lysosomal enzyme activity in macrophage-like cells on Day 7 and thereafter. During chondrolysis and bone remodeling, these cells were present in a perivascular location. Osteoclasts also exhibited strong reactivity for the lysosomal enzymes. Due to its characteristic temporal appearance during development of endochondral bone, arysulfatase may be used as a marker enzyme for chondrolysis and bone resorption.

Original languageEnglish (US)
Pages (from-to)89-98
Number of pages10
JournalDevelopmental Biology
Volume85
Issue number1
DOIs
StatePublished - Jul 15 1981
Externally publishedYes

Fingerprint

Bone Development
Cartilage
Bone Marrow
Bone and Bones
Acid Phosphatase
Arylsulfatases
Enzymes
Bone Remodeling
Glucuronidase
Bone Matrix
Osteoclasts
Bone Resorption
Osteogenesis
Hypertrophy
Macrophages
Cell Proliferation

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Activity and distribution of lysosomal enzymes during collagenous matrix-induced cartilage, bone, and bone marrow development. / Rath, N. C.; Hand, A. R.; Reddi, A Hari.

In: Developmental Biology, Vol. 85, No. 1, 15.07.1981, p. 89-98.

Research output: Contribution to journalArticle

@article{e651c24b741a4c1eb938ce0b5dbae87c,
title = "Activity and distribution of lysosomal enzymes during collagenous matrix-induced cartilage, bone, and bone marrow development",
abstract = "The appearance of the lysosomal enzymes acid phosphatase, arylsulfatase, and β-glucuronidase was studied during endochondral bone and bone marrow formation induced by implantation of demineralized bone matrix. The activities of acid phosphatase and β-glucuronidase gradually increased from the stage of mesenchymal cell proliferation on Day 3 onward to reach a peak on Day 13, during maximal bone remodeling. However, arysulfatase activity exhibited a sharp increase on Day 9, associated with the onset of cartilage hypertrophy and chondrolysis. The peak of arylsulfatase activity was also attained on Day 13. The activities of all three enzymes declined on Day 15 but acid phosphatase again exhibited an increase during hematopoietic bone marrow differentiation on Days 19-21. Histochemical and ultrastructural studies revealed intense lysosomal enzyme activity in macrophage-like cells on Day 7 and thereafter. During chondrolysis and bone remodeling, these cells were present in a perivascular location. Osteoclasts also exhibited strong reactivity for the lysosomal enzymes. Due to its characteristic temporal appearance during development of endochondral bone, arysulfatase may be used as a marker enzyme for chondrolysis and bone resorption.",
author = "Rath, {N. C.} and Hand, {A. R.} and Reddi, {A Hari}",
year = "1981",
month = "7",
day = "15",
doi = "10.1016/0012-1606(81)90238-4",
language = "English (US)",
volume = "85",
pages = "89--98",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Activity and distribution of lysosomal enzymes during collagenous matrix-induced cartilage, bone, and bone marrow development

AU - Rath, N. C.

AU - Hand, A. R.

AU - Reddi, A Hari

PY - 1981/7/15

Y1 - 1981/7/15

N2 - The appearance of the lysosomal enzymes acid phosphatase, arylsulfatase, and β-glucuronidase was studied during endochondral bone and bone marrow formation induced by implantation of demineralized bone matrix. The activities of acid phosphatase and β-glucuronidase gradually increased from the stage of mesenchymal cell proliferation on Day 3 onward to reach a peak on Day 13, during maximal bone remodeling. However, arysulfatase activity exhibited a sharp increase on Day 9, associated with the onset of cartilage hypertrophy and chondrolysis. The peak of arylsulfatase activity was also attained on Day 13. The activities of all three enzymes declined on Day 15 but acid phosphatase again exhibited an increase during hematopoietic bone marrow differentiation on Days 19-21. Histochemical and ultrastructural studies revealed intense lysosomal enzyme activity in macrophage-like cells on Day 7 and thereafter. During chondrolysis and bone remodeling, these cells were present in a perivascular location. Osteoclasts also exhibited strong reactivity for the lysosomal enzymes. Due to its characteristic temporal appearance during development of endochondral bone, arysulfatase may be used as a marker enzyme for chondrolysis and bone resorption.

AB - The appearance of the lysosomal enzymes acid phosphatase, arylsulfatase, and β-glucuronidase was studied during endochondral bone and bone marrow formation induced by implantation of demineralized bone matrix. The activities of acid phosphatase and β-glucuronidase gradually increased from the stage of mesenchymal cell proliferation on Day 3 onward to reach a peak on Day 13, during maximal bone remodeling. However, arysulfatase activity exhibited a sharp increase on Day 9, associated with the onset of cartilage hypertrophy and chondrolysis. The peak of arylsulfatase activity was also attained on Day 13. The activities of all three enzymes declined on Day 15 but acid phosphatase again exhibited an increase during hematopoietic bone marrow differentiation on Days 19-21. Histochemical and ultrastructural studies revealed intense lysosomal enzyme activity in macrophage-like cells on Day 7 and thereafter. During chondrolysis and bone remodeling, these cells were present in a perivascular location. Osteoclasts also exhibited strong reactivity for the lysosomal enzymes. Due to its characteristic temporal appearance during development of endochondral bone, arysulfatase may be used as a marker enzyme for chondrolysis and bone resorption.

UR - http://www.scopus.com/inward/record.url?scp=0019881462&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019881462&partnerID=8YFLogxK

U2 - 10.1016/0012-1606(81)90238-4

DO - 10.1016/0012-1606(81)90238-4

M3 - Article

VL - 85

SP - 89

EP - 98

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

IS - 1

ER -