Activation of the Wnt/Beta-catenin signaling pathway during oral carcinogenesis process is not influenced by the absence of galectin-3 in mice

Juliana Moreira De Almeida Sant'Ana, Roger Chammas, Fu-Tong Liu, Suely Nonogaki, Sérgio Vitorino Cardoso, Adriano Mota Loyola, Paulo Rogério De Faria

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background/Aim: Galectin-3 has been associated with activated Wnt pathway, translocating beta-catenin into the nucleus. However, it is still unknown whether this lectin drives the Wnt signaling activation in lesions from galectin-3-deficient (Gal3-/-) mice. The purpose was to study beta-catenin expression in tongue lesions from Galo3-/- and wild-type (Gal3+/+) mice and the status of Wnt signaling. Materials and Methods: Twenty Gal3-/- and Gal3+/+ male mice were challenged with 4-nitroquinolin-1-oxide and killed at week 16 and 32. Tongues were processed and stained with H&E to detect dysplasias and carcinomas. An imunohistochemical assay was performed to evaluate beta-catenin expression. Results: Carcinomas were more evident in Gal3+/+ than Gal3 -/- mice (55.5% vs. 28.5%, respectively; p>0.05). Elevated expression of non-membranous beta-catenin was observed in dysplasias and carcinomas from both groups (p>0.05). Conclusion: Absence of galectin-3 does not interfere in the pattern of beta-catenin expression and therefore in the mediation of the Wnt signaling pathway.

Original languageEnglish (US)
Pages (from-to)2805-2811
Number of pages7
JournalAnticancer Research
Volume31
Issue number9
StatePublished - Sep 2011

Fingerprint

Galectin 3
Wnt Signaling Pathway
beta Catenin
Carcinogenesis
Carcinoma
Tongue
4-Nitroquinoline-1-oxide
Lectins

Keywords

  • Beta-catenin
  • Immunohistochemistry
  • Mice
  • Oral carcinogenesis
  • Tongue

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Sant'Ana, J. M. D. A., Chammas, R., Liu, F-T., Nonogaki, S., Cardoso, S. V., Loyola, A. M., & De Faria, P. R. (2011). Activation of the Wnt/Beta-catenin signaling pathway during oral carcinogenesis process is not influenced by the absence of galectin-3 in mice. Anticancer Research, 31(9), 2805-2811.

Activation of the Wnt/Beta-catenin signaling pathway during oral carcinogenesis process is not influenced by the absence of galectin-3 in mice. / Sant'Ana, Juliana Moreira De Almeida; Chammas, Roger; Liu, Fu-Tong; Nonogaki, Suely; Cardoso, Sérgio Vitorino; Loyola, Adriano Mota; De Faria, Paulo Rogério.

In: Anticancer Research, Vol. 31, No. 9, 09.2011, p. 2805-2811.

Research output: Contribution to journalArticle

Sant'Ana, JMDA, Chammas, R, Liu, F-T, Nonogaki, S, Cardoso, SV, Loyola, AM & De Faria, PR 2011, 'Activation of the Wnt/Beta-catenin signaling pathway during oral carcinogenesis process is not influenced by the absence of galectin-3 in mice', Anticancer Research, vol. 31, no. 9, pp. 2805-2811.
Sant'Ana, Juliana Moreira De Almeida ; Chammas, Roger ; Liu, Fu-Tong ; Nonogaki, Suely ; Cardoso, Sérgio Vitorino ; Loyola, Adriano Mota ; De Faria, Paulo Rogério. / Activation of the Wnt/Beta-catenin signaling pathway during oral carcinogenesis process is not influenced by the absence of galectin-3 in mice. In: Anticancer Research. 2011 ; Vol. 31, No. 9. pp. 2805-2811.
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abstract = "Background/Aim: Galectin-3 has been associated with activated Wnt pathway, translocating beta-catenin into the nucleus. However, it is still unknown whether this lectin drives the Wnt signaling activation in lesions from galectin-3-deficient (Gal3-/-) mice. The purpose was to study beta-catenin expression in tongue lesions from Galo3-/- and wild-type (Gal3+/+) mice and the status of Wnt signaling. Materials and Methods: Twenty Gal3-/- and Gal3+/+ male mice were challenged with 4-nitroquinolin-1-oxide and killed at week 16 and 32. Tongues were processed and stained with H&E to detect dysplasias and carcinomas. An imunohistochemical assay was performed to evaluate beta-catenin expression. Results: Carcinomas were more evident in Gal3+/+ than Gal3 -/- mice (55.5{\%} vs. 28.5{\%}, respectively; p>0.05). Elevated expression of non-membranous beta-catenin was observed in dysplasias and carcinomas from both groups (p>0.05). Conclusion: Absence of galectin-3 does not interfere in the pattern of beta-catenin expression and therefore in the mediation of the Wnt signaling pathway.",
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