Abstract
Background/Aim: Galectin-3 has been associated with activated Wnt pathway, translocating beta-catenin into the nucleus. However, it is still unknown whether this lectin drives the Wnt signaling activation in lesions from galectin-3-deficient (Gal3-/-) mice. The purpose was to study beta-catenin expression in tongue lesions from Galo3-/- and wild-type (Gal3+/+) mice and the status of Wnt signaling. Materials and Methods: Twenty Gal3-/- and Gal3+/+ male mice were challenged with 4-nitroquinolin-1-oxide and killed at week 16 and 32. Tongues were processed and stained with H&E to detect dysplasias and carcinomas. An imunohistochemical assay was performed to evaluate beta-catenin expression. Results: Carcinomas were more evident in Gal3+/+ than Gal3 -/- mice (55.5% vs. 28.5%, respectively; p>0.05). Elevated expression of non-membranous beta-catenin was observed in dysplasias and carcinomas from both groups (p>0.05). Conclusion: Absence of galectin-3 does not interfere in the pattern of beta-catenin expression and therefore in the mediation of the Wnt signaling pathway.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2805-2811 |
| Number of pages | 7 |
| Journal | Anticancer Research |
| Volume | 31 |
| Issue number | 9 |
| State | Published - Sep 2011 |
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Keywords
- Beta-catenin
- Immunohistochemistry
- Mice
- Oral carcinogenesis
- Tongue
ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Activation of the Wnt/Beta-catenin signaling pathway during oral carcinogenesis process is not influenced by the absence of galectin-3 in mice. / Sant'Ana, Juliana Moreira De Almeida; Chammas, Roger; Liu, Fu-Tong; Nonogaki, Suely; Cardoso, Sérgio Vitorino; Loyola, Adriano Mota; De Faria, Paulo Rogério.
In: Anticancer Research, Vol. 31, No. 9, 09.2011, p. 2805-2811.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Activation of the Wnt/Beta-catenin signaling pathway during oral carcinogenesis process is not influenced by the absence of galectin-3 in mice
AU - Sant'Ana, Juliana Moreira De Almeida
AU - Chammas, Roger
AU - Liu, Fu-Tong
AU - Nonogaki, Suely
AU - Cardoso, Sérgio Vitorino
AU - Loyola, Adriano Mota
AU - De Faria, Paulo Rogério
PY - 2011/9
Y1 - 2011/9
N2 - Background/Aim: Galectin-3 has been associated with activated Wnt pathway, translocating beta-catenin into the nucleus. However, it is still unknown whether this lectin drives the Wnt signaling activation in lesions from galectin-3-deficient (Gal3-/-) mice. The purpose was to study beta-catenin expression in tongue lesions from Galo3-/- and wild-type (Gal3+/+) mice and the status of Wnt signaling. Materials and Methods: Twenty Gal3-/- and Gal3+/+ male mice were challenged with 4-nitroquinolin-1-oxide and killed at week 16 and 32. Tongues were processed and stained with H&E to detect dysplasias and carcinomas. An imunohistochemical assay was performed to evaluate beta-catenin expression. Results: Carcinomas were more evident in Gal3+/+ than Gal3 -/- mice (55.5% vs. 28.5%, respectively; p>0.05). Elevated expression of non-membranous beta-catenin was observed in dysplasias and carcinomas from both groups (p>0.05). Conclusion: Absence of galectin-3 does not interfere in the pattern of beta-catenin expression and therefore in the mediation of the Wnt signaling pathway.
AB - Background/Aim: Galectin-3 has been associated with activated Wnt pathway, translocating beta-catenin into the nucleus. However, it is still unknown whether this lectin drives the Wnt signaling activation in lesions from galectin-3-deficient (Gal3-/-) mice. The purpose was to study beta-catenin expression in tongue lesions from Galo3-/- and wild-type (Gal3+/+) mice and the status of Wnt signaling. Materials and Methods: Twenty Gal3-/- and Gal3+/+ male mice were challenged with 4-nitroquinolin-1-oxide and killed at week 16 and 32. Tongues were processed and stained with H&E to detect dysplasias and carcinomas. An imunohistochemical assay was performed to evaluate beta-catenin expression. Results: Carcinomas were more evident in Gal3+/+ than Gal3 -/- mice (55.5% vs. 28.5%, respectively; p>0.05). Elevated expression of non-membranous beta-catenin was observed in dysplasias and carcinomas from both groups (p>0.05). Conclusion: Absence of galectin-3 does not interfere in the pattern of beta-catenin expression and therefore in the mediation of the Wnt signaling pathway.
KW - Beta-catenin
KW - Immunohistochemistry
KW - Mice
KW - Oral carcinogenesis
KW - Tongue
UR - http://www.scopus.com/inward/record.url?scp=80051646301&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80051646301&partnerID=8YFLogxK
M3 - Article
C2 - 21868523
AN - SCOPUS:80051646301
VL - 31
SP - 2805
EP - 2811
JO - Anticancer Research
JF - Anticancer Research
SN - 0250-7005
IS - 9
ER -