Activation of the Wnt/β-catenin signaling reporter in developing mouse olfactory nerve layer marks a specialized subgroup of olfactory ensheathing cells

Ya Zhou Wang, Andrei Molotkov, Lanying Song, Yunhong Li, David E Pleasure, Chengji Zhou

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Wnt reporter TOPgal mice carry a β-galactosidase (βgal) gene under the control of the Wnt/β-catenin signaling responsive elements. We found that the intensely immunolabeled βgal+ cells were co-immunolabeled with Nestin and formed a tangentially oriented single-cell layer in the "connecting or docking zone" where the olfactory sensory axons attached to the brain surface during mid-gestation. During early postnatal development, βgal+ cells were located in the inner olfactory nerve layer (ONLi) and co-labeled with olfactory ensheathing cell (OEC) markers S100β and NPY but not with lineage-specific markers for neurons, oligodendrocytes, astrocytes, and microglia, demonstrating that the TOPgal marked a subpopulation of OECs. By confocal microscopy, we found that TOPgal activated processes extended along the developing glomerulus and formed multiple tunnel-like structures that ensheathe and bridge olfactory sensory axonal bundles from ONLi to the glomerulus, which may play a key role in glomerulus formation and convergent sorting of the peripheral olfactory axons.

Original languageEnglish (US)
Pages (from-to)3157-3168
Number of pages12
JournalDevelopmental Dynamics
Volume237
Issue number11
DOIs
StatePublished - Nov 2008

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Olfactory Nerve
Catenins
Galactosidases
Axons
Nestin
Oligodendroglia
Microglia
Confocal Microscopy
Astrocytes
Neurons
Pregnancy
Brain
Genes

Keywords

  • Convergent sorting
  • Glomerulus formation
  • Nestin
  • NPY
  • Olfactory ensheathing cells (OECs)
  • Olfactory nerve layer (ONL)
  • Olfactory sensory axons
  • S100β
  • TOPgal transgenic mice
  • Wnt/β-catenin reporter

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Activation of the Wnt/β-catenin signaling reporter in developing mouse olfactory nerve layer marks a specialized subgroup of olfactory ensheathing cells. / Wang, Ya Zhou; Molotkov, Andrei; Song, Lanying; Li, Yunhong; Pleasure, David E; Zhou, Chengji.

In: Developmental Dynamics, Vol. 237, No. 11, 11.2008, p. 3157-3168.

Research output: Contribution to journalArticle

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