Abstract
Wnt reporter TOPgal mice carry a β-galactosidase (βgal) gene under the control of the Wnt/β-catenin signaling responsive elements. We found that the intensely immunolabeled βgal+ cells were co-immunolabeled with Nestin and formed a tangentially oriented single-cell layer in the "connecting or docking zone" where the olfactory sensory axons attached to the brain surface during mid-gestation. During early postnatal development, βgal+ cells were located in the inner olfactory nerve layer (ONLi) and co-labeled with olfactory ensheathing cell (OEC) markers S100β and NPY but not with lineage-specific markers for neurons, oligodendrocytes, astrocytes, and microglia, demonstrating that the TOPgal marked a subpopulation of OECs. By confocal microscopy, we found that TOPgal activated processes extended along the developing glomerulus and formed multiple tunnel-like structures that ensheathe and bridge olfactory sensory axonal bundles from ONLi to the glomerulus, which may play a key role in glomerulus formation and convergent sorting of the peripheral olfactory axons.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 3157-3168 |
| Number of pages | 12 |
| Journal | Developmental Dynamics |
| Volume | 237 |
| Issue number | 11 |
| DOIs | |
| State | Published - Nov 2008 |
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Keywords
- Convergent sorting
- Glomerulus formation
- Nestin
- NPY
- Olfactory ensheathing cells (OECs)
- Olfactory nerve layer (ONL)
- Olfactory sensory axons
- S100β
- TOPgal transgenic mice
- Wnt/β-catenin reporter
ASJC Scopus subject areas
- Developmental Biology
Cite this
Activation of the Wnt/β-catenin signaling reporter in developing mouse olfactory nerve layer marks a specialized subgroup of olfactory ensheathing cells. / Wang, Ya Zhou; Molotkov, Andrei; Song, Lanying; Li, Yunhong; Pleasure, David E; Zhou, Chengji.
In: Developmental Dynamics, Vol. 237, No. 11, 11.2008, p. 3157-3168.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Activation of the Wnt/β-catenin signaling reporter in developing mouse olfactory nerve layer marks a specialized subgroup of olfactory ensheathing cells
AU - Wang, Ya Zhou
AU - Molotkov, Andrei
AU - Song, Lanying
AU - Li, Yunhong
AU - Pleasure, David E
AU - Zhou, Chengji
PY - 2008/11
Y1 - 2008/11
N2 - Wnt reporter TOPgal mice carry a β-galactosidase (βgal) gene under the control of the Wnt/β-catenin signaling responsive elements. We found that the intensely immunolabeled βgal+ cells were co-immunolabeled with Nestin and formed a tangentially oriented single-cell layer in the "connecting or docking zone" where the olfactory sensory axons attached to the brain surface during mid-gestation. During early postnatal development, βgal+ cells were located in the inner olfactory nerve layer (ONLi) and co-labeled with olfactory ensheathing cell (OEC) markers S100β and NPY but not with lineage-specific markers for neurons, oligodendrocytes, astrocytes, and microglia, demonstrating that the TOPgal marked a subpopulation of OECs. By confocal microscopy, we found that TOPgal activated processes extended along the developing glomerulus and formed multiple tunnel-like structures that ensheathe and bridge olfactory sensory axonal bundles from ONLi to the glomerulus, which may play a key role in glomerulus formation and convergent sorting of the peripheral olfactory axons.
AB - Wnt reporter TOPgal mice carry a β-galactosidase (βgal) gene under the control of the Wnt/β-catenin signaling responsive elements. We found that the intensely immunolabeled βgal+ cells were co-immunolabeled with Nestin and formed a tangentially oriented single-cell layer in the "connecting or docking zone" where the olfactory sensory axons attached to the brain surface during mid-gestation. During early postnatal development, βgal+ cells were located in the inner olfactory nerve layer (ONLi) and co-labeled with olfactory ensheathing cell (OEC) markers S100β and NPY but not with lineage-specific markers for neurons, oligodendrocytes, astrocytes, and microglia, demonstrating that the TOPgal marked a subpopulation of OECs. By confocal microscopy, we found that TOPgal activated processes extended along the developing glomerulus and formed multiple tunnel-like structures that ensheathe and bridge olfactory sensory axonal bundles from ONLi to the glomerulus, which may play a key role in glomerulus formation and convergent sorting of the peripheral olfactory axons.
KW - Convergent sorting
KW - Glomerulus formation
KW - Nestin
KW - NPY
KW - Olfactory ensheathing cells (OECs)
KW - Olfactory nerve layer (ONL)
KW - Olfactory sensory axons
KW - S100β
KW - TOPgal transgenic mice
KW - Wnt/β-catenin reporter
UR - http://www.scopus.com/inward/record.url?scp=55549131561&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=55549131561&partnerID=8YFLogxK
U2 - 10.1002/dvdy.21712
DO - 10.1002/dvdy.21712
M3 - Article
C2 - 18816448
AN - SCOPUS:55549131561
VL - 237
SP - 3157
EP - 3168
JO - Developmental Dynamics
JF - Developmental Dynamics
SN - 1058-8388
IS - 11
ER -