Activation of the AIDS retrovirus promoter by the cellular transcription factor, Sp1

Katherine A. Jones, James T. Kadonaga, Paul A Luciw, Robert Tjian

Research output: Contribution to journalArticle

404 Citations (Scopus)

Abstract

The nature and position of transcriptional control elements responsible for the expression of genes encoded by the retrovirus associated with acquired immune deficiency syndrome (AIDS) have not been precisely defined. In this study it is shown that the mammalian Sp1 transcription factor binds to promoter sequences within the AIDS retrovirus long terminal repeat (LTR) and activates RNA synthesis five- to eightfold in reconstituted reactions in vitro. Experiments in which regions of DNA were protected from added reagents by specifically bound proteins (footprinting) indicated that the upstream promoter region of the AIDS virus LTR lies between -45 and -77 (relative to the RNA start site, +1) and contains three tandem, closely spaced Sp1 binding sites of variable affinity. Base-substitution mutations targeted to one or all three Sp1 binding sites were found both to eliminate the binding of Sp1 and to cause up to a tenfold reduction in transcriptional efficiency in vitro. These findings suggest that one important component of the AIDS virus transcriptional control region interacts with a cellular transcription factor, Sp1, and that this factor must function in conjunction with transcriptional elements located downstream of the RNA cap site to mediate the response of the LTR to viral trans-activation.

Original languageEnglish (US)
Pages (from-to)755-759
Number of pages5
JournalScience
Volume232
Issue number4751
StatePublished - 1986
Externally publishedYes

Fingerprint

Sp1 Transcription Factor
Terminal Repeat Sequences
Retroviridae
Acquired Immunodeficiency Syndrome
Protein Footprinting
RNA Caps
Binding Sites
HIV
RNA
Virus Activation
Genetic Promoter Regions
Gene Expression
Mutation
DNA
In Vitro Techniques

ASJC Scopus subject areas

  • General

Cite this

Jones, K. A., Kadonaga, J. T., Luciw, P. A., & Tjian, R. (1986). Activation of the AIDS retrovirus promoter by the cellular transcription factor, Sp1. Science, 232(4751), 755-759.

Activation of the AIDS retrovirus promoter by the cellular transcription factor, Sp1. / Jones, Katherine A.; Kadonaga, James T.; Luciw, Paul A; Tjian, Robert.

In: Science, Vol. 232, No. 4751, 1986, p. 755-759.

Research output: Contribution to journalArticle

Jones, KA, Kadonaga, JT, Luciw, PA & Tjian, R 1986, 'Activation of the AIDS retrovirus promoter by the cellular transcription factor, Sp1', Science, vol. 232, no. 4751, pp. 755-759.
Jones, Katherine A. ; Kadonaga, James T. ; Luciw, Paul A ; Tjian, Robert. / Activation of the AIDS retrovirus promoter by the cellular transcription factor, Sp1. In: Science. 1986 ; Vol. 232, No. 4751. pp. 755-759.
@article{e29e24054f8c4a0ca4485ab0e58c1a34,
title = "Activation of the AIDS retrovirus promoter by the cellular transcription factor, Sp1",
abstract = "The nature and position of transcriptional control elements responsible for the expression of genes encoded by the retrovirus associated with acquired immune deficiency syndrome (AIDS) have not been precisely defined. In this study it is shown that the mammalian Sp1 transcription factor binds to promoter sequences within the AIDS retrovirus long terminal repeat (LTR) and activates RNA synthesis five- to eightfold in reconstituted reactions in vitro. Experiments in which regions of DNA were protected from added reagents by specifically bound proteins (footprinting) indicated that the upstream promoter region of the AIDS virus LTR lies between -45 and -77 (relative to the RNA start site, +1) and contains three tandem, closely spaced Sp1 binding sites of variable affinity. Base-substitution mutations targeted to one or all three Sp1 binding sites were found both to eliminate the binding of Sp1 and to cause up to a tenfold reduction in transcriptional efficiency in vitro. These findings suggest that one important component of the AIDS virus transcriptional control region interacts with a cellular transcription factor, Sp1, and that this factor must function in conjunction with transcriptional elements located downstream of the RNA cap site to mediate the response of the LTR to viral trans-activation.",
author = "Jones, {Katherine A.} and Kadonaga, {James T.} and Luciw, {Paul A} and Robert Tjian",
year = "1986",
language = "English (US)",
volume = "232",
pages = "755--759",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "4751",

}

TY - JOUR

T1 - Activation of the AIDS retrovirus promoter by the cellular transcription factor, Sp1

AU - Jones, Katherine A.

AU - Kadonaga, James T.

AU - Luciw, Paul A

AU - Tjian, Robert

PY - 1986

Y1 - 1986

N2 - The nature and position of transcriptional control elements responsible for the expression of genes encoded by the retrovirus associated with acquired immune deficiency syndrome (AIDS) have not been precisely defined. In this study it is shown that the mammalian Sp1 transcription factor binds to promoter sequences within the AIDS retrovirus long terminal repeat (LTR) and activates RNA synthesis five- to eightfold in reconstituted reactions in vitro. Experiments in which regions of DNA were protected from added reagents by specifically bound proteins (footprinting) indicated that the upstream promoter region of the AIDS virus LTR lies between -45 and -77 (relative to the RNA start site, +1) and contains three tandem, closely spaced Sp1 binding sites of variable affinity. Base-substitution mutations targeted to one or all three Sp1 binding sites were found both to eliminate the binding of Sp1 and to cause up to a tenfold reduction in transcriptional efficiency in vitro. These findings suggest that one important component of the AIDS virus transcriptional control region interacts with a cellular transcription factor, Sp1, and that this factor must function in conjunction with transcriptional elements located downstream of the RNA cap site to mediate the response of the LTR to viral trans-activation.

AB - The nature and position of transcriptional control elements responsible for the expression of genes encoded by the retrovirus associated with acquired immune deficiency syndrome (AIDS) have not been precisely defined. In this study it is shown that the mammalian Sp1 transcription factor binds to promoter sequences within the AIDS retrovirus long terminal repeat (LTR) and activates RNA synthesis five- to eightfold in reconstituted reactions in vitro. Experiments in which regions of DNA were protected from added reagents by specifically bound proteins (footprinting) indicated that the upstream promoter region of the AIDS virus LTR lies between -45 and -77 (relative to the RNA start site, +1) and contains three tandem, closely spaced Sp1 binding sites of variable affinity. Base-substitution mutations targeted to one or all three Sp1 binding sites were found both to eliminate the binding of Sp1 and to cause up to a tenfold reduction in transcriptional efficiency in vitro. These findings suggest that one important component of the AIDS virus transcriptional control region interacts with a cellular transcription factor, Sp1, and that this factor must function in conjunction with transcriptional elements located downstream of the RNA cap site to mediate the response of the LTR to viral trans-activation.

UR - http://www.scopus.com/inward/record.url?scp=0022541602&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022541602&partnerID=8YFLogxK

M3 - Article

C2 - 3008338

AN - SCOPUS:0022541602

VL - 232

SP - 755

EP - 759

JO - Science

JF - Science

SN - 0036-8075

IS - 4751

ER -