Activation of Rat Basophilic Leukemia Cells by ε{lunate}BP, an IgE-Binding Endogenous Lectin

Riaz I. Zuberi, Luciano G. Frigeri, Fu-Tong Liu

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

IgE-binding protein (ε{lunate}BP) is a β-galactoside-binding animal lectin identified by its affinity for IgE. We have reported that ε{lunate}BP also binds the mast cell high-affinity IgE receptor (Fcε{lunate}RI), via lectin-carbohydrate interaction. We have now studied the physiological significance of ε{lunate}BP-IgE-Fcε{lunate}RI interactions in mast cell activation using rat basophilic leukemia (RBL) cells as the model system. We report here that both unsensitized and IgE-sensitized RBL cells are activated upon exposure to ε{lunate}BP-coated surfaces. Activation of RBL cells by the lectin ε{lunate}BP can be significantly inhibited by appropriate saccharides. Exposure of RBL cells to ε{lunate}BP-coated surfaces caused cell spreading similar to that caused from adherence to fibronectin-coated surfaces. However, ε{lunate}BP by itself caused mediator release whereas fibronectin only potentiated antigen-mediated activation of RBL cells. Under appropriate conditions, ε{lunate}BP, therefore, has the potential to activate mast cells culminating in augmentation of an inflammatory response.

Original languageEnglish (US)
Pages (from-to)1-12
Number of pages12
JournalCellular Immunology
Volume156
Issue number1
DOIs
StatePublished - Jun 1994

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Lectins
Immunoglobulin E
Leukemia
Mast Cells
Fibronectins
Galectin 3
IgE Receptors
Galactosides
Carbohydrates
Antigens

ASJC Scopus subject areas

  • Immunology
  • Cell Biology

Cite this

Activation of Rat Basophilic Leukemia Cells by ε{lunate}BP, an IgE-Binding Endogenous Lectin. / Zuberi, Riaz I.; Frigeri, Luciano G.; Liu, Fu-Tong.

In: Cellular Immunology, Vol. 156, No. 1, 06.1994, p. 1-12.

Research output: Contribution to journalArticle

Zuberi, Riaz I. ; Frigeri, Luciano G. ; Liu, Fu-Tong. / Activation of Rat Basophilic Leukemia Cells by ε{lunate}BP, an IgE-Binding Endogenous Lectin. In: Cellular Immunology. 1994 ; Vol. 156, No. 1. pp. 1-12.
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