Activation of NFATc3 down-regulates the β1 subunit of large conductance, calcium-activated K+ channels in arterial smooth muscle and contributes to hypertension

Madeline Nieves-Cintrón, Gregory C. Amberg, C. Blake Nichols, Jeffery D. Molkentin, Luis F. Santana

Research output: Contribution to journalArticlepeer-review

89 Scopus citations

Abstract

Large conductance, Ca2+-activated K+ (BK) channels modulate the excitability and contractile state of arterial smooth muscle. Recently, we demonstrated that during hypertension, expression of the accessory β1 subunit was decreased relative to the pore-forming α subunit of the BK channel. Reduced β1 subunit expression resulted in BK channels with impaired function due to lowered sensitivity to Ca2+. Here, we tested the hypothesis that activation of the calcineurin/NFATc3 signaling pathway down-regulates β1 expression during angiotensin II-induced hypertension. Consistent with this hypothesis, we found that in vivo administration of angiotensin II-activated calcineurin/NFATc3 signaling in arterial smooth muscle. During angiotensin II infusion, arterial smooth muscle BK channel function was decreased in wild type (WT) but not in NFATc3 null (NFATc3-/-) mice. Accordingly, β1 expression was decreased in WT but not in NFATc3 -/- arteries. Angiotensin II-induced down-regulation of the β1 subunit required Ca2+ influx via L-type Ca2+ channels. However, in the absence of angiotensin II, moderate elevation of [Ca 2+]i alone was not sufficient to activate NFAT transcriptional activity and, thus, decrease β1 subunit expression. Importantly, angiotensin II infusion increased systemic blood pressure to a lower extent in NFATc3-/- than in WT mice, indicating that this transcription factor is required for the development of severe hypertension during chronic angiotensin II signaling activation. We conclude that activation of calcineurin and NFATc3 during sustained angiotensin II signaling down-regulates the expression of the β1 subunit of the BK channel, which in turn contributes to arterial dysfunction and the development of hypertension.

Original languageEnglish (US)
Pages (from-to)3231-3240
Number of pages10
JournalJournal of Biological Chemistry
Volume282
Issue number5
DOIs
StatePublished - Jan 2 2007
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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