Activation of neurons in trigeminal caudalis by noxious oral acidic or salt stimuli is not reduced by amiloride

Satoko Sudo, Makoto Sudo, Christopher T. Simons, Jean Marc Dessirier, Mirela Iodi Carstens, Earl Carstens

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We investigated the possible role of amiloride-sensitive ion channels of the ENaC/DEGenerin superfamily in the activation of trigeminal nociceptive neurons elicited by noxious chemical stimulation of the oral mucosa using two methodologies, single-unit recording and c-fos immunohistochemistry. In pentobarbital-anesthetized rats, single-unit recordings were made from neurons in superficial laminae of dorsomedial trigeminal subnucleus caudalis (Vc) that responded to noxious thermal and chemical stimuli applied to the dorsal tongue. Successive application of each of three chemicals (250 mM pentanoic acid, n=6 units; 250 mM citric acid, n=8; 5 M NaCl, n=6) evoked responses that were not affected following topical application of amiloride (1 mM). In separate experiments, pentobarbital-anesthetized rats received one of the following stimuli delivered to the dorsal tongue: 250 mM pentanoic acid (n=6); 1 mM amiloride followed by 250 mM pentanoic (N=6); 5 M NaCl (n=5); or 1 mM amiloride followed by 5 M NaCl (n=5). Two hours later they were perfused with 4% paraformaldehyde and the brain stems processed for c-fos immunoreactivity. Both pentanoic acid and 5 M NaCl evoked similar numbers and patterns of fos-like immunoreactivity (FLI) in dorsomedial Vc and other brain stem regions, with no significant difference in counts of FLI in animals pretreated with amiloride. These results suggest that amiloride-sensitive Na+ channels are not essential in mediating the activation of intraoral trigeminal nociceptors.

Original languageEnglish (US)
Pages (from-to)237-243
Number of pages7
JournalBrain Research
Volume969
Issue number1-2
DOIs
StatePublished - Apr 18 2003

Fingerprint

Amiloride
Salts
Neurons
Nociceptors
Pentobarbital
Tongue
Brain Stem
Degenerin Sodium Channels
Pentanoic Acids
Chemical Stimulation
Mouth Mucosa
Citric Acid
Hot Temperature
Immunohistochemistry

Keywords

  • Acid-sensing ion channel
  • Amiloride
  • Chemonociception
  • ENac/DEGenerin
  • Oral imitation
  • Trigeminal caudalis

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Sudo, S., Sudo, M., Simons, C. T., Dessirier, J. M., Carstens, M. I., & Carstens, E. (2003). Activation of neurons in trigeminal caudalis by noxious oral acidic or salt stimuli is not reduced by amiloride. Brain Research, 969(1-2), 237-243. https://doi.org/10.1016/S0006-8993(03)02341-2

Activation of neurons in trigeminal caudalis by noxious oral acidic or salt stimuli is not reduced by amiloride. / Sudo, Satoko; Sudo, Makoto; Simons, Christopher T.; Dessirier, Jean Marc; Carstens, Mirela Iodi; Carstens, Earl.

In: Brain Research, Vol. 969, No. 1-2, 18.04.2003, p. 237-243.

Research output: Contribution to journalArticle

Sudo, S, Sudo, M, Simons, CT, Dessirier, JM, Carstens, MI & Carstens, E 2003, 'Activation of neurons in trigeminal caudalis by noxious oral acidic or salt stimuli is not reduced by amiloride', Brain Research, vol. 969, no. 1-2, pp. 237-243. https://doi.org/10.1016/S0006-8993(03)02341-2
Sudo, Satoko ; Sudo, Makoto ; Simons, Christopher T. ; Dessirier, Jean Marc ; Carstens, Mirela Iodi ; Carstens, Earl. / Activation of neurons in trigeminal caudalis by noxious oral acidic or salt stimuli is not reduced by amiloride. In: Brain Research. 2003 ; Vol. 969, No. 1-2. pp. 237-243.
@article{fe8da3fef00940dc95f6cb76fabc6547,
title = "Activation of neurons in trigeminal caudalis by noxious oral acidic or salt stimuli is not reduced by amiloride",
abstract = "We investigated the possible role of amiloride-sensitive ion channels of the ENaC/DEGenerin superfamily in the activation of trigeminal nociceptive neurons elicited by noxious chemical stimulation of the oral mucosa using two methodologies, single-unit recording and c-fos immunohistochemistry. In pentobarbital-anesthetized rats, single-unit recordings were made from neurons in superficial laminae of dorsomedial trigeminal subnucleus caudalis (Vc) that responded to noxious thermal and chemical stimuli applied to the dorsal tongue. Successive application of each of three chemicals (250 mM pentanoic acid, n=6 units; 250 mM citric acid, n=8; 5 M NaCl, n=6) evoked responses that were not affected following topical application of amiloride (1 mM). In separate experiments, pentobarbital-anesthetized rats received one of the following stimuli delivered to the dorsal tongue: 250 mM pentanoic acid (n=6); 1 mM amiloride followed by 250 mM pentanoic (N=6); 5 M NaCl (n=5); or 1 mM amiloride followed by 5 M NaCl (n=5). Two hours later they were perfused with 4{\%} paraformaldehyde and the brain stems processed for c-fos immunoreactivity. Both pentanoic acid and 5 M NaCl evoked similar numbers and patterns of fos-like immunoreactivity (FLI) in dorsomedial Vc and other brain stem regions, with no significant difference in counts of FLI in animals pretreated with amiloride. These results suggest that amiloride-sensitive Na+ channels are not essential in mediating the activation of intraoral trigeminal nociceptors.",
keywords = "Acid-sensing ion channel, Amiloride, Chemonociception, ENac/DEGenerin, Oral imitation, Trigeminal caudalis",
author = "Satoko Sudo and Makoto Sudo and Simons, {Christopher T.} and Dessirier, {Jean Marc} and Carstens, {Mirela Iodi} and Earl Carstens",
year = "2003",
month = "4",
day = "18",
doi = "10.1016/S0006-8993(03)02341-2",
language = "English (US)",
volume = "969",
pages = "237--243",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Activation of neurons in trigeminal caudalis by noxious oral acidic or salt stimuli is not reduced by amiloride

AU - Sudo, Satoko

AU - Sudo, Makoto

AU - Simons, Christopher T.

AU - Dessirier, Jean Marc

AU - Carstens, Mirela Iodi

AU - Carstens, Earl

PY - 2003/4/18

Y1 - 2003/4/18

N2 - We investigated the possible role of amiloride-sensitive ion channels of the ENaC/DEGenerin superfamily in the activation of trigeminal nociceptive neurons elicited by noxious chemical stimulation of the oral mucosa using two methodologies, single-unit recording and c-fos immunohistochemistry. In pentobarbital-anesthetized rats, single-unit recordings were made from neurons in superficial laminae of dorsomedial trigeminal subnucleus caudalis (Vc) that responded to noxious thermal and chemical stimuli applied to the dorsal tongue. Successive application of each of three chemicals (250 mM pentanoic acid, n=6 units; 250 mM citric acid, n=8; 5 M NaCl, n=6) evoked responses that were not affected following topical application of amiloride (1 mM). In separate experiments, pentobarbital-anesthetized rats received one of the following stimuli delivered to the dorsal tongue: 250 mM pentanoic acid (n=6); 1 mM amiloride followed by 250 mM pentanoic (N=6); 5 M NaCl (n=5); or 1 mM amiloride followed by 5 M NaCl (n=5). Two hours later they were perfused with 4% paraformaldehyde and the brain stems processed for c-fos immunoreactivity. Both pentanoic acid and 5 M NaCl evoked similar numbers and patterns of fos-like immunoreactivity (FLI) in dorsomedial Vc and other brain stem regions, with no significant difference in counts of FLI in animals pretreated with amiloride. These results suggest that amiloride-sensitive Na+ channels are not essential in mediating the activation of intraoral trigeminal nociceptors.

AB - We investigated the possible role of amiloride-sensitive ion channels of the ENaC/DEGenerin superfamily in the activation of trigeminal nociceptive neurons elicited by noxious chemical stimulation of the oral mucosa using two methodologies, single-unit recording and c-fos immunohistochemistry. In pentobarbital-anesthetized rats, single-unit recordings were made from neurons in superficial laminae of dorsomedial trigeminal subnucleus caudalis (Vc) that responded to noxious thermal and chemical stimuli applied to the dorsal tongue. Successive application of each of three chemicals (250 mM pentanoic acid, n=6 units; 250 mM citric acid, n=8; 5 M NaCl, n=6) evoked responses that were not affected following topical application of amiloride (1 mM). In separate experiments, pentobarbital-anesthetized rats received one of the following stimuli delivered to the dorsal tongue: 250 mM pentanoic acid (n=6); 1 mM amiloride followed by 250 mM pentanoic (N=6); 5 M NaCl (n=5); or 1 mM amiloride followed by 5 M NaCl (n=5). Two hours later they were perfused with 4% paraformaldehyde and the brain stems processed for c-fos immunoreactivity. Both pentanoic acid and 5 M NaCl evoked similar numbers and patterns of fos-like immunoreactivity (FLI) in dorsomedial Vc and other brain stem regions, with no significant difference in counts of FLI in animals pretreated with amiloride. These results suggest that amiloride-sensitive Na+ channels are not essential in mediating the activation of intraoral trigeminal nociceptors.

KW - Acid-sensing ion channel

KW - Amiloride

KW - Chemonociception

KW - ENac/DEGenerin

KW - Oral imitation

KW - Trigeminal caudalis

UR - http://www.scopus.com/inward/record.url?scp=0037453268&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037453268&partnerID=8YFLogxK

U2 - 10.1016/S0006-8993(03)02341-2

DO - 10.1016/S0006-8993(03)02341-2

M3 - Article

C2 - 12676384

AN - SCOPUS:0037453268

VL - 969

SP - 237

EP - 243

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1-2

ER -