Activation of a plant plasma membrane Ca2+ channel by TGα1, a heterotrimeric G protein α-subunit homologue

Gilad S. Aharon, Angela C Gelli, Wayne A. Snedden, Eduardo Blumwald

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Wild-type and GTPase-deficient recombinant TGα1 were used along patch-clamp techniques to study the role of heterotrimeric G proteins in the regulation of the hyperpolarized active tomato plasma membrane Ca2+ channel. Recombinant α-subunits induced an increase in channel activity as shown by the increase in channel events and the mean open probability of the channel. Our results suggest a membrane-delimited pathway involving heterotrimeric G proteins in Ca2+ channel activation.

Original languageEnglish (US)
Pages (from-to)17-21
Number of pages5
JournalFEBS Letters
Volume424
Issue number1-2
DOIs
StatePublished - Mar 6 1998
Externally publishedYes

Fingerprint

Heterotrimeric GTP-Binding Proteins
Protein Subunits
Cell membranes
Ion Channels
Chemical activation
Cell Membrane
GTP Phosphohydrolases
Clamping devices
Patch-Clamp Techniques
Lycopersicon esculentum
Membranes

Keywords

  • Heterotrimeric G protein
  • Plasma membrane Ca channel
  • Signal transduction
  • TGα1

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Activation of a plant plasma membrane Ca2+ channel by TGα1, a heterotrimeric G protein α-subunit homologue. / Aharon, Gilad S.; Gelli, Angela C; Snedden, Wayne A.; Blumwald, Eduardo.

In: FEBS Letters, Vol. 424, No. 1-2, 06.03.1998, p. 17-21.

Research output: Contribution to journalArticle

Aharon, Gilad S. ; Gelli, Angela C ; Snedden, Wayne A. ; Blumwald, Eduardo. / Activation of a plant plasma membrane Ca2+ channel by TGα1, a heterotrimeric G protein α-subunit homologue. In: FEBS Letters. 1998 ; Vol. 424, No. 1-2. pp. 17-21.
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