Activation-dependent surface expression of LOX-1 in human platelets

M. Chen, M. Kakutani, T. Naruko, M. Ueda, S. Narumiya, T. Masaki, T. Sawamura

Research output: Contribution to journalArticle

109 Citations (Scopus)

Abstract

Lectin-like oxidized LDL receptor-1 (LOX-1) was initially identified as an oxidized LDL receptor in aortic endothelial cells. Here we identified LOX-1 mRNA and protein in human platelets in addition to recent findings on the expression in macrophages and smooth muscle cells. The presence of LOX-1 was further confirmed in the megakaryocytic cell lines. Flow cytometric analyses revealed that LOX-1 was exposed on the surface of platelets in an activation-dependent manner. Consistently, the activation-dependent binding of OxLDL to platelets was mostly inhibited by anti-LOX-1 antibody. Immunohistochemistry of the atherosclerotic plaque from a patient with unstable angina pectoris (UAP) revealed accumulation of LOX-1 protein at the site of thrombus. As LOX-1 recognizes and binds activated platelets, exposure of LOX-1 on activated platelets surface might assist thrombosis formation.

Original languageEnglish (US)
Pages (from-to)153-158
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume282
Issue number1
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Oxidized LDL Receptors
Platelets
LDL Lipoproteins
Blood Platelets
Chemical activation
Thrombosis
Cells
Macrophages
Unstable Angina
Endothelial cells
Platelet Activation
Atherosclerotic Plaques
Lectins
Smooth Muscle Myocytes
Muscle
Proteins
Endothelial Cells
Immunohistochemistry

Keywords

  • Human platelet
  • LOX-1
  • Oxidized LDL

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Chen, M., Kakutani, M., Naruko, T., Ueda, M., Narumiya, S., Masaki, T., & Sawamura, T. (2001). Activation-dependent surface expression of LOX-1 in human platelets. Biochemical and Biophysical Research Communications, 282(1), 153-158. https://doi.org/10.1006/bbrc.2001.4516

Activation-dependent surface expression of LOX-1 in human platelets. / Chen, M.; Kakutani, M.; Naruko, T.; Ueda, M.; Narumiya, S.; Masaki, T.; Sawamura, T.

In: Biochemical and Biophysical Research Communications, Vol. 282, No. 1, 2001, p. 153-158.

Research output: Contribution to journalArticle

Chen, M, Kakutani, M, Naruko, T, Ueda, M, Narumiya, S, Masaki, T & Sawamura, T 2001, 'Activation-dependent surface expression of LOX-1 in human platelets', Biochemical and Biophysical Research Communications, vol. 282, no. 1, pp. 153-158. https://doi.org/10.1006/bbrc.2001.4516
Chen, M. ; Kakutani, M. ; Naruko, T. ; Ueda, M. ; Narumiya, S. ; Masaki, T. ; Sawamura, T. / Activation-dependent surface expression of LOX-1 in human platelets. In: Biochemical and Biophysical Research Communications. 2001 ; Vol. 282, No. 1. pp. 153-158.
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