Activation and regulation of the Spc1 stress-activated protein kinase in Schizosaccharomyces pombe

Geneviève Degols, Kazuhiro Shiozaki, Paul Russell

Research output: Contribution to journalArticle

243 Citations (Scopus)

Abstract

Spc1, an osmotic-stress-stimulated mitogen-activated protein kinase (MAPK) homolog in the fission yeast Schizosaccharomyces pombe, is required for the induction of mitosis and survival in high-osmolarity conditions. Spc1, also known as Sty1, is activated by Wis1 MAPK kinase and inhibited by Pyp1 tyrosine phosphatase. Spc1 is most closely related to Saccharomyces cerevisiae Hog1 and mammalian p38 kinases. Whereas Hog1 is specifically responsive to osmotic stress, we report here that Spc1 is activated by multiple forms of stress, including high temperature and oxidative stress. In this regard Spc1 is more similar to mammalian p38. Activation of Spc1 is crucial for survival of various forms of stress. Spc1 regulates expression of genes encoding stress-related proteins such as glycerol-3-phosphate dehydrogenase (gpd1+) and trehalose-6-phosphate synthase (tps1+). Spc1 also promotes expression of pyp2+, which encodes a tyrosine phosphatase postulated as a negative regulator of Spc1. This proposal is supported by the finding that Spc1 associates with Pyp2 in vivo and that the amount of Spc1 tyrosine phosphorylation is lower in a Pyp2-overproducing strain than in the wild type. Moreover, the level of stress-stimulated gpd1+ expression is higher in Δpyp2 mutants than in the wild type. These findings demonstrate that Spc1 promotes expression of genes involved in stress survival and that Spc1-regulated transcription of one of these genes, pyp2+, serves to attenuate Spc1 activity. This mechanism of regulation may be commonly employed to modulate MAPK signal transduction pathways in eukaryotic species.

Original languageEnglish (US)
Pages (from-to)2870-2877
Number of pages8
JournalMolecular and Cellular Biology
Volume16
Issue number6
StatePublished - 1996
Externally publishedYes

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Schizosaccharomyces
Heat-Shock Proteins
Protein Kinases
Tyrosine
Osmotic Pressure
Mitogen-Activated Protein Kinases
Phosphoric Monoester Hydrolases
Glycerolphosphate Dehydrogenase
Gene Expression
Mitogen-Activated Protein Kinase Kinases
Mitosis
Osmolar Concentration
Saccharomyces cerevisiae
Signal Transduction
Oxidative Stress
Phosphotransferases
Phosphorylation
Temperature
Genes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Activation and regulation of the Spc1 stress-activated protein kinase in Schizosaccharomyces pombe. / Degols, Geneviève; Shiozaki, Kazuhiro; Russell, Paul.

In: Molecular and Cellular Biology, Vol. 16, No. 6, 1996, p. 2870-2877.

Research output: Contribution to journalArticle

Degols, Geneviève ; Shiozaki, Kazuhiro ; Russell, Paul. / Activation and regulation of the Spc1 stress-activated protein kinase in Schizosaccharomyces pombe. In: Molecular and Cellular Biology. 1996 ; Vol. 16, No. 6. pp. 2870-2877.
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