Activated monocytes and platelet-monocyte aggregates in patients with sickle cell disease

Theodore Wun, Miguel Cordoba, Arun Rangaswami, Anthony W. Cheung, Teresa Paglieroni

Research output: Contribution to journalArticle

87 Scopus citations

Abstract

Tumour necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) increase endothelial surface receptors that mediate the adherence of sickle erythrocytes to the endothelium. Increased circulating levels of these cytokines have been found in patients with sickle cell disease (SCD). Monocytes are a source of both of these inflammatory mediators; we therefore determined whether circulating monocytes were activated in SCD, as defined by intracellular expression of these cytokines. Blood was also assayed for the presence of platelet-monocyte aggregates (PMAs), as platelet adherence is one possible mechanism for monocyte activation. The median percentages of monocytes expressing intracellular TNF-α and IL-1β in SCD patients were 6.8 (2.8-17.3) [median (range)] and 14.1 (1.3-44.8), respectively. In African-American controls the corresponding percentages were 0.3 (0.1-0.5) and 0.4 (0.1-3.0), and in Caucasians 0.2 (0.1-0.5) and 0.8 (0.8-1.9) (P < 0.001, Kruskal-Wallis). The mean percentage (± SD) of PMA was 14.0 ± 8.3 for Caucasian controls, 25.7 ± 7.3 for African-American controls, and 45.7 ± 21.6 for patients with SCD (P < 0.001, RM ANOVA; P ± 0.05, Newman-Keuls posthoc test). We conclude that there are increased circulating PMAs and monocyte activation in patients with SCD.

Original languageEnglish (US)
Pages (from-to)81-88
Number of pages8
JournalClinical and Laboratory Haematology
Volume24
Issue number2
DOIs
StatePublished - 2002

Keywords

  • Inflammation
  • Monocyte
  • Platelet
  • Sickle cell

ASJC Scopus subject areas

  • Hematology

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