Abstract
We describe the detection and characterization of an activated c-N-ras allele from a γ-radiation-induced canine acute nonlymphocytic leukemia (ANLL). The activated allele was detected by use of the NIH3T3 transfection/transformation assay. The leukemia DNA had a transforming activity of 0.0125 foci/μg. By the use of a double anti-ras antibody enzyme-linked immunoblot assay, we have dissected the lesion within the activated c-N-ras allele. Aspartic acid has been substituted for the normal glycine at position 12 in the activated p21(c-N-ras). The expression of the mutant p21(ras) has also been detected in an in vivo passage of the radiation-induced canine ANLL from which the activated c-N-ras allele was isolated. We have demonstrated sufficient homology between canine c-N-ras genes and the human cDNA c-N-ras clone, p6a1, that allows this probe to be used in Southern blotting of canine tissues. In addition, anti-ras antibodies generated against both murine and human ras antigens are capable of detecting canine p21(ras) species.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 198-206 |
| Number of pages | 9 |
| Journal | Radiation Research |
| Volume | 117 |
| Issue number | 2 |
| State | Published - 1989 |
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ASJC Scopus subject areas
- Agricultural and Biological Sciences (miscellaneous)
- Biophysics
- Radiology Nuclear Medicine and imaging
- Radiation
Cite this
Activated c-N-ras in radiation-induced acute nonlymphocytic leukemia : twelfth codon aspartic acid. / Gumerlock, P. H.; Meyers, Frederick J; Foster, B. A.; Kawakami, T. G.; deVere White, Ralph W.
In: Radiation Research, Vol. 117, No. 2, 1989, p. 198-206.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Activated c-N-ras in radiation-induced acute nonlymphocytic leukemia
T2 - twelfth codon aspartic acid
AU - Gumerlock, P. H.
AU - Meyers, Frederick J
AU - Foster, B. A.
AU - Kawakami, T. G.
AU - deVere White, Ralph W
PY - 1989
Y1 - 1989
N2 - We describe the detection and characterization of an activated c-N-ras allele from a γ-radiation-induced canine acute nonlymphocytic leukemia (ANLL). The activated allele was detected by use of the NIH3T3 transfection/transformation assay. The leukemia DNA had a transforming activity of 0.0125 foci/μg. By the use of a double anti-ras antibody enzyme-linked immunoblot assay, we have dissected the lesion within the activated c-N-ras allele. Aspartic acid has been substituted for the normal glycine at position 12 in the activated p21(c-N-ras). The expression of the mutant p21(ras) has also been detected in an in vivo passage of the radiation-induced canine ANLL from which the activated c-N-ras allele was isolated. We have demonstrated sufficient homology between canine c-N-ras genes and the human cDNA c-N-ras clone, p6a1, that allows this probe to be used in Southern blotting of canine tissues. In addition, anti-ras antibodies generated against both murine and human ras antigens are capable of detecting canine p21(ras) species.
AB - We describe the detection and characterization of an activated c-N-ras allele from a γ-radiation-induced canine acute nonlymphocytic leukemia (ANLL). The activated allele was detected by use of the NIH3T3 transfection/transformation assay. The leukemia DNA had a transforming activity of 0.0125 foci/μg. By the use of a double anti-ras antibody enzyme-linked immunoblot assay, we have dissected the lesion within the activated c-N-ras allele. Aspartic acid has been substituted for the normal glycine at position 12 in the activated p21(c-N-ras). The expression of the mutant p21(ras) has also been detected in an in vivo passage of the radiation-induced canine ANLL from which the activated c-N-ras allele was isolated. We have demonstrated sufficient homology between canine c-N-ras genes and the human cDNA c-N-ras clone, p6a1, that allows this probe to be used in Southern blotting of canine tissues. In addition, anti-ras antibodies generated against both murine and human ras antigens are capable of detecting canine p21(ras) species.
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UR - http://www.scopus.com/inward/citedby.url?scp=0024563659&partnerID=8YFLogxK
M3 - Article
C2 - 2646654
AN - SCOPUS:0024563659
VL - 117
SP - 198
EP - 206
JO - Radiation Research
JF - Radiation Research
SN - 0033-7587
IS - 2
ER -