Activated c-N-ras in radiation-induced acute nonlymphocytic leukemia: twelfth codon aspartic acid

P. H. Gumerlock, Frederick J Meyers, B. A. Foster, T. G. Kawakami, Ralph W deVere White

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

We describe the detection and characterization of an activated c-N-ras allele from a γ-radiation-induced canine acute nonlymphocytic leukemia (ANLL). The activated allele was detected by use of the NIH3T3 transfection/transformation assay. The leukemia DNA had a transforming activity of 0.0125 foci/μg. By the use of a double anti-ras antibody enzyme-linked immunoblot assay, we have dissected the lesion within the activated c-N-ras allele. Aspartic acid has been substituted for the normal glycine at position 12 in the activated p21(c-N-ras). The expression of the mutant p21(ras) has also been detected in an in vivo passage of the radiation-induced canine ANLL from which the activated c-N-ras allele was isolated. We have demonstrated sufficient homology between canine c-N-ras genes and the human cDNA c-N-ras clone, p6a1, that allows this probe to be used in Southern blotting of canine tissues. In addition, anti-ras antibodies generated against both murine and human ras antigens are capable of detecting canine p21(ras) species.

Original languageEnglish (US)
Pages (from-to)198-206
Number of pages9
JournalRadiation Research
Volume117
Issue number2
StatePublished - 1989

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ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biophysics
  • Radiology Nuclear Medicine and imaging
  • Radiation

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