The development of neuronal responses to γ-aminobutyric acid (GABA) was examined using intracellular recording techniques in area CA1 of rabbit hippocampal slices maintained in vitro. Micro-application of GABA (via pressure ejection) in stratum pyramidale in slices from mature rabbits (age 1 month) evoked a hyperpolarization of CA1 pyramidal neurons. The reversal potential (Erev) for this response was approximately -70 mV. In contrast, local application of GABA into stratum pyramidale in slices from immature rabbits (age 7-10 days) produced a depolarizing response with an Erev of approximately -54 mV. The relationship between these findings and the development of inhibitory synaptic activity in rabbit hippocampus is discussed.
- pressure ejection
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