Actin-myosin viscoelastic flow in the keratocyte lamellipod

Boris Rubinstein, Maxime F. Fournier, Ken Jacobson, Alexander B. Verkhovsky, Alex Mogilner

Research output: Contribution to journalArticle

122 Scopus citations

Abstract

The lamellipod, the locomotory region of migratory cells, is shaped by the balance of protrusion and contraction. The latter is the result of myosin-generated centripetal flow of the viscoelastic actin network. Recently, quantitative flow data was obtained, yet there is no detailed theory explaining the flow in a realistic geometry. We introduce models of viscoelastic actin mechanics and myosin transport and solve the model equations numerically for the flat, fan-shaped lamellipodial domain of keratocytes. The solutions demonstrate that in the rapidly crawling cell, myosin concentrates at the rear boundary and pulls the actin network inward, so the centripetal actin flow is very slow at the front, and faster at the rear and at the sides. The computed flow and respective traction forces compare well with the experimental data. We also calculate the graded protrusion at the cell boundary necessary to maintain the cell shape and make a number of other testable predictions. We discuss model implications for the cell shape, speed, and bi-stability.

Original languageEnglish (US)
Pages (from-to)1853-1863
Number of pages11
JournalBiophysical Journal
Volume97
Issue number7
DOIs
StatePublished - Oct 7 2009

ASJC Scopus subject areas

  • Biophysics

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    Rubinstein, B., Fournier, M. F., Jacobson, K., Verkhovsky, A. B., & Mogilner, A. (2009). Actin-myosin viscoelastic flow in the keratocyte lamellipod. Biophysical Journal, 97(7), 1853-1863. https://doi.org/10.1016/j.bpj.2009.07.020