Actin and α-actinin orchestrate the assembly and maturation of nascent adhesions in a myosin II motor-independent manner

Colin K. Choi, Miguel Vicente-Manzanares, Jessica Zareno, Leanna A. Whitmore, Alex Mogilner, Alan Rick Horwitz

Research output: Contribution to journalArticle

476 Citations (Scopus)

Abstract

Using two-colour imaging and high resolution TIRF microscopy, we investigated the assembly and maturation of nascent adhesions in migrating cells. We show that nascent adhesions assemble and are stable within the lamellipodium. The assembly is independent of myosin II but its rate is proportional to the protrusion rate and requires actin polymerization. At the lamellipodium back, the nascent adhesions either disassemble or mature through growth and elongation. Maturation occurs along an α-actinin-actin template that elongates centripetally from nascent adhesions. α-Actinin mediates the formation of the template and organization of adhesions associated with actin filaments, suggesting that actin crosslinking has a major role in this process. Adhesion maturation also requires myosin II. Rescue of a myosin IIA knockdown with an actin-bound but motor-inhibited mutant of myosin IIA shows that the actin crosslinking function of myosin II mediates initial adhesion maturation. From these studies, we have developed a model for adhesion assembly that clarifies the relative contributions of myosin II and actin polymerization and organization.

Original languageEnglish (US)
Pages (from-to)1039-1050
Number of pages12
JournalNature Cell Biology
Volume10
Issue number9
DOIs
StatePublished - 2008

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Myosin Type II
Actinin
Actins
Nonmuscle Myosin Type IIA
Pseudopodia
Polymerization
Actin Cytoskeleton
Microscopy
Color
Growth

ASJC Scopus subject areas

  • Cell Biology

Cite this

Choi, C. K., Vicente-Manzanares, M., Zareno, J., Whitmore, L. A., Mogilner, A., & Horwitz, A. R. (2008). Actin and α-actinin orchestrate the assembly and maturation of nascent adhesions in a myosin II motor-independent manner. Nature Cell Biology, 10(9), 1039-1050. https://doi.org/10.1038/ncb1763

Actin and α-actinin orchestrate the assembly and maturation of nascent adhesions in a myosin II motor-independent manner. / Choi, Colin K.; Vicente-Manzanares, Miguel; Zareno, Jessica; Whitmore, Leanna A.; Mogilner, Alex; Horwitz, Alan Rick.

In: Nature Cell Biology, Vol. 10, No. 9, 2008, p. 1039-1050.

Research output: Contribution to journalArticle

Choi, CK, Vicente-Manzanares, M, Zareno, J, Whitmore, LA, Mogilner, A & Horwitz, AR 2008, 'Actin and α-actinin orchestrate the assembly and maturation of nascent adhesions in a myosin II motor-independent manner', Nature Cell Biology, vol. 10, no. 9, pp. 1039-1050. https://doi.org/10.1038/ncb1763
Choi, Colin K. ; Vicente-Manzanares, Miguel ; Zareno, Jessica ; Whitmore, Leanna A. ; Mogilner, Alex ; Horwitz, Alan Rick. / Actin and α-actinin orchestrate the assembly and maturation of nascent adhesions in a myosin II motor-independent manner. In: Nature Cell Biology. 2008 ; Vol. 10, No. 9. pp. 1039-1050.
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