Acrylamide causes preimplantation abnormalities in embryos and induces chromatin-adducts in male germ cells of mice

N. Holland, T. Ahlborn, Ken W Turteltaub, C. Markee, D. Moore, A. J. Wyrobek, M. T. Smith

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Acrylamide, a known male postmeiotic germ cell mutagen, caused a dose- dependent increase in the frequency of morphologic abnormalities in preimplantation embryos. Single-cell eggs, growth retardation, and blastomere lysis were detected after paternal treatment with acrylamide (10 to 50 mg/kg, 5 d). The major effects were seen at weeks 1 to 3 after male treatment, with the highest level of abnormalities at the first week (>90% vs. 5% in control). The frequency of abnormal four-day embryos was similar to preimplantation loss assessed at 15 to 16 d p.c. A > 100-fold elevation of chromatin adducts in sperm was observed during 1st and 2nd week after treatment, after which adduct levels decreased to baseline level. However, morphologic defects in embryos are not fully explained by the spermatid adduct curve. These findings demonstrate the effects of paternal exposure to acrylamide on preimplantation development and indicate a potential risk to the offspring of men exposed to acrylamide.

Original languageEnglish (US)
Pages (from-to)167-178
Number of pages12
JournalReproductive Toxicology
Volume13
Issue number3
DOIs
StatePublished - May 1999
Externally publishedYes

Fingerprint

Acrylamide
Germ Cells
Chromatin
Embryonic Structures
Cells
Paternal Exposure
Blastomeres
Spermatids
Mutagens
Cell growth
Blastocyst
Eggs
Spermatozoa
Therapeutics
Defects
Growth

Keywords

  • Acrylamide
  • Chromatin-adducts
  • Genotoxicity
  • Preimplantation abnormalities
  • Sperm

ASJC Scopus subject areas

  • Toxicology

Cite this

Acrylamide causes preimplantation abnormalities in embryos and induces chromatin-adducts in male germ cells of mice. / Holland, N.; Ahlborn, T.; Turteltaub, Ken W; Markee, C.; Moore, D.; Wyrobek, A. J.; Smith, M. T.

In: Reproductive Toxicology, Vol. 13, No. 3, 05.1999, p. 167-178.

Research output: Contribution to journalArticle

Holland, N. ; Ahlborn, T. ; Turteltaub, Ken W ; Markee, C. ; Moore, D. ; Wyrobek, A. J. ; Smith, M. T. / Acrylamide causes preimplantation abnormalities in embryos and induces chromatin-adducts in male germ cells of mice. In: Reproductive Toxicology. 1999 ; Vol. 13, No. 3. pp. 167-178.
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