Achieving high detection sensitivity (14 zmol) of biomolecular ions in bioaerosol mass spectrometry

Scott C. Russell, Gregg Czerwieniec, Carlito B Lebrilla, Paul Steele, Vincent Riot, Keith Coffee, Matthias Frank, Eric E. Gard

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Bioaerosol mass spectrometry (BAMS) performs single-cell analysis in real time. However, the specificity of BAMS mass signatures has been limited by low sensitivity at high masses. To increase the mass range and sensitivity of BAMS, a novel design was developed that utilizes a linear flight tube with delayed extraction and an electrostatic ion guide. This study quantifies the sensitivity limits of the novel BAMS design and evaluates the feasibility of BAMS to detect higher mass biomarkers from single cells. All experiments were carried out using MALDI aerosol particles that were nebulized from solution. Sensitivity was assessed by generating particles with decreasing amounts of analyte via serial dilutions. The amount of analyte contained within each particle was calculated based on particle size, density, and molarity of the analyte within solution. A variety of biomolecular ions were studied and signals obtained from particles containing 300 zmol of maltopentaose, 132 zmol of α-cyclodextrin, and 14 zmol (∼8400 molecules) of gramicidin S are reported. The detection of 14 zmol of gramicidin S is to the best of our knowledge a record in sensitivity for MALDI TOF-MS.

Original languageEnglish (US)
Pages (from-to)4734-4741
Number of pages8
JournalAnalytical Chemistry
Volume77
Issue number15
DOIs
StatePublished - Aug 1 2005

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Mass spectrometry
Ions
Gramicidin
Cyclodextrins
Biomarkers
Aerosols
Dilution
Electrostatics
Particle size
Molecules
Experiments

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Russell, S. C., Czerwieniec, G., Lebrilla, C. B., Steele, P., Riot, V., Coffee, K., ... Gard, E. E. (2005). Achieving high detection sensitivity (14 zmol) of biomolecular ions in bioaerosol mass spectrometry. Analytical Chemistry, 77(15), 4734-4741. https://doi.org/10.1021/ac048202r

Achieving high detection sensitivity (14 zmol) of biomolecular ions in bioaerosol mass spectrometry. / Russell, Scott C.; Czerwieniec, Gregg; Lebrilla, Carlito B; Steele, Paul; Riot, Vincent; Coffee, Keith; Frank, Matthias; Gard, Eric E.

In: Analytical Chemistry, Vol. 77, No. 15, 01.08.2005, p. 4734-4741.

Research output: Contribution to journalArticle

Russell, SC, Czerwieniec, G, Lebrilla, CB, Steele, P, Riot, V, Coffee, K, Frank, M & Gard, EE 2005, 'Achieving high detection sensitivity (14 zmol) of biomolecular ions in bioaerosol mass spectrometry', Analytical Chemistry, vol. 77, no. 15, pp. 4734-4741. https://doi.org/10.1021/ac048202r
Russell SC, Czerwieniec G, Lebrilla CB, Steele P, Riot V, Coffee K et al. Achieving high detection sensitivity (14 zmol) of biomolecular ions in bioaerosol mass spectrometry. Analytical Chemistry. 2005 Aug 1;77(15):4734-4741. https://doi.org/10.1021/ac048202r
Russell, Scott C. ; Czerwieniec, Gregg ; Lebrilla, Carlito B ; Steele, Paul ; Riot, Vincent ; Coffee, Keith ; Frank, Matthias ; Gard, Eric E. / Achieving high detection sensitivity (14 zmol) of biomolecular ions in bioaerosol mass spectrometry. In: Analytical Chemistry. 2005 ; Vol. 77, No. 15. pp. 4734-4741.
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