Acetylation/Deacetylation Reactions of T-2, Acetyl T-2, HT-2, and Acetyl HT-2 Toxins in Bovine Rumen Fluid in Vitro

Craig E. Munger, G. Wayne Ivie, Ronald J. Christopher, Bruce D. Hammock, Timothy D. Phillips

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

A tritiated preparation of the trichothecene mycotoxin, T-2 toxin, underwent both acetylation and deacetylation reactions when incubated with bovine rumen fluid in vitro. Products from incubations of T-2 in rumen fluid included acetyl T-2, HT-2, and acetyl HT-2. Direct studies with tritiated samples of each of these metabolites confirmed their relatively facile interconversion in the rumen. Studies with [3H]HT-2 under conditions of inhibited esterase activity (added diisopropyl fluorophosphate) showed that acetylation is preferred at C-3 vs. C-4. Studies with [3H]acetyl T-2 indicated that deacetylation similarly occurs with greater rapidity at C-3. There were no indications that ester hydrolysis of these trichothecenes occurred at C-8 or C-15 or that they were subjected to epoxide reduction reactions. These data suggest that acetylation of T-2 and other trichothecenes in the rumen in situ may ultimately result in the absorption of more lipophilic metabolites whose toxicological and residual properties are at present unknown.

Original languageEnglish (US)
Pages (from-to)354-358
Number of pages5
JournalJournal of Agricultural and Food Chemistry
Volume35
Issue number3
DOIs
StatePublished - May 1 1987

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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