Oligodendroglia prepared from calf cerebral white matter were incubated with [3- 14C] acetoacetate (AcAc), D-[3 14C](-)-beta-hydroxybutyrate (OHbut) or 3H 2O. Addition to the medium of the ATP citrate oxaloacetate lyase inhibitor (-)-hydroxycitrate diminished sterol labelling from OHbut and 3H 2O, but caused 4 fold stimulation of sterol labelling from AcAc. The discrepancy between results with the two ketone bodies indicates the existence in oligodendroglia of an extramitochondrial pathway for conversion of AcAc but not OHbut to acetyl CoA. Acetoacetyl CoA synthetase, the first enzyme in this pathway, was more than 20-fold enriched in oligodendroglia over whole brain.
|Original language||English (US)|
|Number of pages||4|
|Journal||Journal of Neurochemistry|
|State||Published - 1979|
ASJC Scopus subject areas
- Cellular and Molecular Neuroscience