Acetoacetate and D-(-)-beta-hydroxybutyrate as precursors for sterol synthesis by calf oligodendrocytes in suspension culture: extramitochondrial pathway for acetoacetate metabolism

David E Pleasure, C. Lichtman, S. Eastman, M. Lieb, O. Abramsky, D. Silberberg

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Oligodendroglia prepared from calf cerebral white matter were incubated with [3- 14C] acetoacetate (AcAc), D-[3 14C](-)-beta-hydroxybutyrate (OHbut) or 3H 2O. Addition to the medium of the ATP citrate oxaloacetate lyase inhibitor (-)-hydroxycitrate diminished sterol labelling from OHbut and 3H 2O, but caused 4 fold stimulation of sterol labelling from AcAc. The discrepancy between results with the two ketone bodies indicates the existence in oligodendroglia of an extramitochondrial pathway for conversion of AcAc but not OHbut to acetyl CoA. Acetoacetyl CoA synthetase, the first enzyme in this pathway, was more than 20-fold enriched in oligodendroglia over whole brain.

Original languageEnglish (US)
Pages (from-to)1447-1450
Number of pages4
JournalJournal of Neurochemistry
Volume32
Issue number5
DOIs
StatePublished - 1979
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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