Accurate PCR Detection of Influenza A/B and Respiratory Syncytial Viruses by Use of Cepheid Xpert Flu+RSV Xpress Assay in Point-of-Care Settings: Comparison to Prodesse ProFlu

Daniel M. Cohen, Jennifer Kline, Larissa S. May, Glenn Eric Harnett, Jane Gibson, Stephen Y. Liang, Zubaid Rafique, Carina A. Rodriguez, Kevin M. McGann, Charlotte A. Gaydos, Donna Mayne, David Phillips, Jason Cohen

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The Xpert Flu+RSV Xpress Assay is a fast, automated in vitro diagnostic test for qualitative detection and differentiation of influenza A and B viruses and respiratory syncytial virus (RSV) performed on the Cepheid GeneXpert Xpress System. The objective of this study was to establish performance characteristics of the Xpert Flu+RSV Xpress Assay compared to those of the Prodesse ProFlu+ real-time reverse transcription-PCR (RT-PCR) assay (ProFlu+) for the detection of influenza A and B viruses as well as RSV in a Clinical Laboratory Improvement Amendments (CLIA)-waived (CW) setting. Overall, the assay, using fresh and frozen nasopharyngeal (NP) swabs, demonstrated high concordance with results of the ProFlu+ assay in the combined CW and non-CW settings with positive percent agreements (PPA) (100%, 100%, and 97.1%) and negative percent agreements (NPA) (95.2%, 99.5%, and 99.6%) for influenza A and B viruses and RSV, respectively. In conclusion, this multicenter study using the Cepheid Xpert Flu+RSV Xpress Assay demonstrated high sensitivities and specificities for influenza A and B viruses and RSV in ∼60 min for use at the point-of-care in the CW setting.

Original languageEnglish (US)
JournalJournal of clinical microbiology
Volume56
Issue number2
DOIs
StatePublished - Feb 1 2018

Fingerprint

Point-of-Care Systems
Respiratory Syncytial Viruses
Human Influenza
Influenza B virus
Influenza A virus
Polymerase Chain Reaction
Routine Diagnostic Tests
Reverse Transcription
Multicenter Studies
Sensitivity and Specificity

Keywords

  • CLIA-waived
  • DNA polymerase
  • influenza
  • respiratory syncytial virus

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Accurate PCR Detection of Influenza A/B and Respiratory Syncytial Viruses by Use of Cepheid Xpert Flu+RSV Xpress Assay in Point-of-Care Settings : Comparison to Prodesse ProFlu. / Cohen, Daniel M.; Kline, Jennifer; May, Larissa S.; Harnett, Glenn Eric; Gibson, Jane; Liang, Stephen Y.; Rafique, Zubaid; Rodriguez, Carina A.; McGann, Kevin M.; Gaydos, Charlotte A.; Mayne, Donna; Phillips, David; Cohen, Jason.

In: Journal of clinical microbiology, Vol. 56, No. 2, 01.02.2018.

Research output: Contribution to journalArticle

Cohen, DM, Kline, J, May, LS, Harnett, GE, Gibson, J, Liang, SY, Rafique, Z, Rodriguez, CA, McGann, KM, Gaydos, CA, Mayne, D, Phillips, D & Cohen, J 2018, 'Accurate PCR Detection of Influenza A/B and Respiratory Syncytial Viruses by Use of Cepheid Xpert Flu+RSV Xpress Assay in Point-of-Care Settings: Comparison to Prodesse ProFlu', Journal of clinical microbiology, vol. 56, no. 2. https://doi.org/10.1128/JCM.01237-17
Cohen, Daniel M. ; Kline, Jennifer ; May, Larissa S. ; Harnett, Glenn Eric ; Gibson, Jane ; Liang, Stephen Y. ; Rafique, Zubaid ; Rodriguez, Carina A. ; McGann, Kevin M. ; Gaydos, Charlotte A. ; Mayne, Donna ; Phillips, David ; Cohen, Jason. / Accurate PCR Detection of Influenza A/B and Respiratory Syncytial Viruses by Use of Cepheid Xpert Flu+RSV Xpress Assay in Point-of-Care Settings : Comparison to Prodesse ProFlu. In: Journal of clinical microbiology. 2018 ; Vol. 56, No. 2.
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