Accurate mass QTOF and MS∧n Ion trap measurements require additional NMR data for plant metabolites de-novo identification

Vladimir Tolstikov, Burkhard Costisella, Wolfram Weckwerth, Baichen Zhang, Oliver Fiehn

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

The de novo identification of plant metabolites was carried out by accurate mass QTOF and MS^n ion trap measurements. Hydrophilic interaction chromatography (HILIC) separation of protein free samples from phloem transport system of cucurbita maxima(pumpkin), coupled with ESI MS and UVD detection allowed to detect more than 70 components. Accurate mass measurement of isolated unknown compounds was done for ESI FTICR MS. When compared to reference compounds, it was found that pumpkin phloem samples have a number of small N-containing molecules like regular and irregular amino acids, amino polyols, monosaccharides, oligomers of the raffinose sugar family and sugar nucleotides.

Original languageEnglish (US)
Title of host publicationProceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages417-418
Number of pages2
StatePublished - 2002
Externally publishedYes
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

ASJC Scopus subject areas

  • Spectroscopy

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    Tolstikov, V., Costisella, B., Weckwerth, W., Zhang, B., & Fiehn, O. (2002). Accurate mass QTOF and MS∧n Ion trap measurements require additional NMR data for plant metabolites de-novo identification. In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics (pp. 417-418)