Accumulation of mutants in "aging" bacterial colonies is due to growth under selection, not stress-induced mutagenesis

Marie Wrande, John R. Roth, Diarmaid Hughes

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Several bacterial systems show behavior interpreted as evidence for stress-induced mutagenesis (adaptive mutation), a postulated process by which nongrowing cells temporarily increase their general mutation rate. Theoretical considerations suggest that periodic stress-induced general mutagenesis would not be advantageous in the long term, due to the high cost of deleterious mutations. Alternative explanations have been tested for very few of the systems used as evidence for stress-induced mutation. In one prominent system, mutants resistant to rifampicin (RifR; rpoB; RNA polymerase) accumulate in cell populations that "age" on solid medium with little net growth. Mutant accumulation was initially attributed to stress-induced general mutagenesis in nongrowing cells. Evidence is presented that these Rif R mutants accumulate because they grow faster than parent cells during the aging period. Direct tests revealed no increase in the frequency of other mutant types during the aging period.

Original languageEnglish (US)
Pages (from-to)11863-11868
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue number33
DOIs
StatePublished - Aug 19 2008

Fingerprint

Mutagenesis
Mutation
Growth
Cell Aging
Mutation Rate
DNA-Directed RNA Polymerases
Rifampin
Costs and Cost Analysis
Population

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Accumulation of mutants in "aging" bacterial colonies is due to growth under selection, not stress-induced mutagenesis. / Wrande, Marie; Roth, John R.; Hughes, Diarmaid.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, No. 33, 19.08.2008, p. 11863-11868.

Research output: Contribution to journalArticle

@article{4e6d4747d1654e2cb51df4d140dfe00e,
title = "Accumulation of mutants in {"}aging{"} bacterial colonies is due to growth under selection, not stress-induced mutagenesis",
abstract = "Several bacterial systems show behavior interpreted as evidence for stress-induced mutagenesis (adaptive mutation), a postulated process by which nongrowing cells temporarily increase their general mutation rate. Theoretical considerations suggest that periodic stress-induced general mutagenesis would not be advantageous in the long term, due to the high cost of deleterious mutations. Alternative explanations have been tested for very few of the systems used as evidence for stress-induced mutation. In one prominent system, mutants resistant to rifampicin (RifR; rpoB; RNA polymerase) accumulate in cell populations that {"}age{"} on solid medium with little net growth. Mutant accumulation was initially attributed to stress-induced general mutagenesis in nongrowing cells. Evidence is presented that these Rif R mutants accumulate because they grow faster than parent cells during the aging period. Direct tests revealed no increase in the frequency of other mutant types during the aging period.",
author = "Marie Wrande and Roth, {John R.} and Diarmaid Hughes",
year = "2008",
month = "8",
day = "19",
doi = "10.1073/pnas.0804739105",
language = "English (US)",
volume = "105",
pages = "11863--11868",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "33",

}

TY - JOUR

T1 - Accumulation of mutants in "aging" bacterial colonies is due to growth under selection, not stress-induced mutagenesis

AU - Wrande, Marie

AU - Roth, John R.

AU - Hughes, Diarmaid

PY - 2008/8/19

Y1 - 2008/8/19

N2 - Several bacterial systems show behavior interpreted as evidence for stress-induced mutagenesis (adaptive mutation), a postulated process by which nongrowing cells temporarily increase their general mutation rate. Theoretical considerations suggest that periodic stress-induced general mutagenesis would not be advantageous in the long term, due to the high cost of deleterious mutations. Alternative explanations have been tested for very few of the systems used as evidence for stress-induced mutation. In one prominent system, mutants resistant to rifampicin (RifR; rpoB; RNA polymerase) accumulate in cell populations that "age" on solid medium with little net growth. Mutant accumulation was initially attributed to stress-induced general mutagenesis in nongrowing cells. Evidence is presented that these Rif R mutants accumulate because they grow faster than parent cells during the aging period. Direct tests revealed no increase in the frequency of other mutant types during the aging period.

AB - Several bacterial systems show behavior interpreted as evidence for stress-induced mutagenesis (adaptive mutation), a postulated process by which nongrowing cells temporarily increase their general mutation rate. Theoretical considerations suggest that periodic stress-induced general mutagenesis would not be advantageous in the long term, due to the high cost of deleterious mutations. Alternative explanations have been tested for very few of the systems used as evidence for stress-induced mutation. In one prominent system, mutants resistant to rifampicin (RifR; rpoB; RNA polymerase) accumulate in cell populations that "age" on solid medium with little net growth. Mutant accumulation was initially attributed to stress-induced general mutagenesis in nongrowing cells. Evidence is presented that these Rif R mutants accumulate because they grow faster than parent cells during the aging period. Direct tests revealed no increase in the frequency of other mutant types during the aging period.

UR - http://www.scopus.com/inward/record.url?scp=50149112177&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=50149112177&partnerID=8YFLogxK

U2 - 10.1073/pnas.0804739105

DO - 10.1073/pnas.0804739105

M3 - Article

C2 - 18701713

AN - SCOPUS:50149112177

VL - 105

SP - 11863

EP - 11868

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 33

ER -