Accumulation of α [14C]aminoisobutyric acid by canine cranial meninges from cerebrospinal fluid in vitro

L. A. O'Tuama, R. J. Tallaksen, M. P. Remler, H. P. Nichols

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Active transport out of CSF has been described for several compounds and is considered to be one of the homeostatic mechanisms by which a tight regulation of the brain extracellular composition is maintained. Considerable interest has therefore centered on defining the mechanisms and sites of CSF transport. In the case of amino acids, the presence of a carrier mediated and energy dependent transport from ventricular CSF was shown and in vitro experiments established a role in this process for the choroid plexus, which showed a concentrative uptake of amino acids. An important extension of this concept was provided by the demonstration of mediated clearance of amino acids from the extraventricular CSF of the spinal and cranial subarachnoid compartments. Since these compartments lack choroid plexus, these observations indicated that other cell types may be sites for the transport of amino acids from CSF. The present study sought to establish whether the meningeal membranes might be such a locus: adult mongrel dogs were used for the experiment. Although the authors found an accumulation of [14]AIB by meninges to be less by 2.6 fold than that of choroid plexus on a unit weight basis, the accumulation of solute by available sites in the total meningeal surface may exceed (considerably) that occurring across choroidal epithelium. The mechanism for removal of the accumulated solute from the meninges is uncertain, but may involve clearance by pial blood vessels.

Original languageEnglish (US)
Pages (from-to)581-583
Number of pages3
JournalJournal of Neurochemistry
Volume24
Issue number3
StatePublished - 1975
Externally publishedYes

Fingerprint

Aminoisobutyric Acids
Cerebrospinal fluid
Meninges
Choroid Plexus
Cerebrospinal Fluid
Canidae
Amino Acids
Active Biological Transport
Blood vessels
Blood Vessels
Brain
Demonstrations
Epithelium
Experiments
Dogs
Membranes
Weights and Measures
In Vitro Techniques
Chemical analysis

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

O'Tuama, L. A., Tallaksen, R. J., Remler, M. P., & Nichols, H. P. (1975). Accumulation of α [14C]aminoisobutyric acid by canine cranial meninges from cerebrospinal fluid in vitro. Journal of Neurochemistry, 24(3), 581-583.

Accumulation of α [14C]aminoisobutyric acid by canine cranial meninges from cerebrospinal fluid in vitro. / O'Tuama, L. A.; Tallaksen, R. J.; Remler, M. P.; Nichols, H. P.

In: Journal of Neurochemistry, Vol. 24, No. 3, 1975, p. 581-583.

Research output: Contribution to journalArticle

O'Tuama, LA, Tallaksen, RJ, Remler, MP & Nichols, HP 1975, 'Accumulation of α [14C]aminoisobutyric acid by canine cranial meninges from cerebrospinal fluid in vitro', Journal of Neurochemistry, vol. 24, no. 3, pp. 581-583.
O'Tuama, L. A. ; Tallaksen, R. J. ; Remler, M. P. ; Nichols, H. P. / Accumulation of α [14C]aminoisobutyric acid by canine cranial meninges from cerebrospinal fluid in vitro. In: Journal of Neurochemistry. 1975 ; Vol. 24, No. 3. pp. 581-583.
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