Accelerator mass spectrometry in the biomedical sciences: applications in low-exposure biomedical and environmental dosimetry

J. S. Felton, Ken W Turteltaub, J. S. Vogel, R. Balhorn, B. L. Gledhill, J. R. Southon, M. W. Caffee, R. C. Finkel, D. E. Nelson, I. D. Proctor, J. C. Davis

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

We are utilizing accelerator mass spectrometry as a sensitive detector for tracking the disposition of radioisotopically labeled molecules in the biomedical sciences. These applications have shown the effectiveness of AMS as a tool to quantify biologically important molecules at extremely low levels. For example, AMS is being used to determine the amount of carcinogen covalently bound to animal DNA (DNA adduct) at levels relevent to human exposure. Detection sensitivities are 1 carcinogen molecule bound in 1011 to 1012 DNA bases, depending on the specific activity of the radiolabeled carcinogen. Studies have been undertaken in our laboratory utilizing heterocyclic amine food-borne carcinogens and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), a potent environmental carcinogen, to study the metabolism of carcinogens at low doses. In addition, AMS is being used to detect the presence of rare proteins (mutant forms of protamine) in human sperm. Approximately l per 106 sperm analyzed contain the rare form of the protamine. Protamine isolated from this small number of cells is being analyzed by AMS, following 14C labeling. Thus, AMS can be used to verify the identity of an extremely small amount of biological material. Furthermore, an additional improvement of 2 orders of magnitude in the sensitivity of biomédical tracer studies is suggested by preliminary work with bacterial hosts depleted in radiocarbon. Other problems in the life sciences where detection sensitivity or sample sizes are limitations should also benefit from AMS. Studies are underway to measure the molecular targeting of cancer chemotherapeutics in human tissue and to pursue applications for receptor biology. We are also applying other candidate isotopes, such as 3H (double labeling with 14C) and 41Ca (bone absorption) to problems in biology. The detection of 36Cl and 26Al have applications for determination of human neutron exposure and understanding neurological toxicity, respectively. The results described here with 14C-labeled molecules coupled with new isotope applications clearly show AMS technology to be an important new tool for the biomedical sciences community.

Original languageEnglish (US)
Pages (from-to)517-523
Number of pages7
JournalNuclear Inst. and Methods in Physics Research, B
Volume52
Issue number3-4
DOIs
StatePublished - Dec 2 1990
Externally publishedYes

Fingerprint

Carcinogens
carcinogens
Alpha Magnetic Spectrometer
Dosimetry
Particle accelerators
dosimeters
Mass spectrometry
accelerators
mass spectroscopy
Protamines
Molecules
Isotopes
Labeling
deoxyribonucleic acid
biology
Environmental Carcinogens
DNA
marking
molecules
DNA Adducts

ASJC Scopus subject areas

  • Surfaces, Coatings and Films
  • Instrumentation
  • Surfaces and Interfaces

Cite this

Accelerator mass spectrometry in the biomedical sciences : applications in low-exposure biomedical and environmental dosimetry. / Felton, J. S.; Turteltaub, Ken W; Vogel, J. S.; Balhorn, R.; Gledhill, B. L.; Southon, J. R.; Caffee, M. W.; Finkel, R. C.; Nelson, D. E.; Proctor, I. D.; Davis, J. C.

In: Nuclear Inst. and Methods in Physics Research, B, Vol. 52, No. 3-4, 02.12.1990, p. 517-523.

Research output: Contribution to journalArticle

Felton, JS, Turteltaub, KW, Vogel, JS, Balhorn, R, Gledhill, BL, Southon, JR, Caffee, MW, Finkel, RC, Nelson, DE, Proctor, ID & Davis, JC 1990, 'Accelerator mass spectrometry in the biomedical sciences: applications in low-exposure biomedical and environmental dosimetry', Nuclear Inst. and Methods in Physics Research, B, vol. 52, no. 3-4, pp. 517-523. https://doi.org/10.1016/0168-583X(90)90469-B
Felton, J. S. ; Turteltaub, Ken W ; Vogel, J. S. ; Balhorn, R. ; Gledhill, B. L. ; Southon, J. R. ; Caffee, M. W. ; Finkel, R. C. ; Nelson, D. E. ; Proctor, I. D. ; Davis, J. C. / Accelerator mass spectrometry in the biomedical sciences : applications in low-exposure biomedical and environmental dosimetry. In: Nuclear Inst. and Methods in Physics Research, B. 1990 ; Vol. 52, No. 3-4. pp. 517-523.
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