Acceleration of regenerate ossification during distraction osteogenesis with recombinant human bone morphogenetic protein-7

Yoshihiko Mizumoto, Timothy Moseley, Michael Drews, Virgil N. Cooper, A Hari Reddi

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Background: Bone-lengthening can be accomplished by means of distraction osteogenesis. In the present study, we examined the effect of a single dose of recombinant human bone morphogenetic protein-7 (rhBMP-7) on the rate of new-bone formation during distraction osteogenesis in the rat. Methods: Fourteen Long-Evans rats were randomized into two groups of seven rats each. An external fixator was applied to the left femur, and a transverse osteotomy was performed. One group was treated with rhBMP-7 in an aqueous solvent, and the other group received the solvent alone and served as the control. rhBMP-7 was administered on the day of surgery by means of a single injection into the osteotomy gap. Distraction was started seven days after surgery at a rate of 0.25 mm every twelve hours. Distraction was continued for twenty days, resulting in a total of 10 mm of lengthening. The regenerate was monitored with use of radiographs and bone-mineral-density measurements at the conclusion of distraction and at two and four weeks after the cessation of distraction. The lengthened femora were harvested, and biomechanical studies were carried out to determine the stiffness and maximum torque to failure. Results: Radiographs showed accelerated regenerate ossification in the BMP-7 group, with a larger amount of new bone compared with the control group. The bone-mineral-density values were dramatically enhanced on Day 20 in the BMP-7 group (103.6 ± 12.6 mg/cm2) compared with the control group (26.2 ± 15.1 mg/cm2). These differences continued to be greater at two and four weeks after the cessation of distraction. Normalized values of stiffness (percent stiffness) reached 76.5% ± 5.4% in the BMP-7 group, compared with 6.6% ± 0.5% in the control group. The percent maximum torque at failure was 81.1% ± 1.2% in the BMP-7 group, compared with 20.8% ± 0.3% in the control group. Conclusion: A single injection of rhBMP-7 at the time of osteotomy surgery stimulated the rate of regenerate ossification and increased bone-mineral density during distraction osteogenesis. The biomechanical properties of the newly formed bone were also increased by BMP-7 injection. Clinical Relevance: The present study demonstrates the benefits of rhBMP-7 on the acceleration of regenerate ossification and indicates its potential utility in clinical situations to reduce the treatment period with an external fixator during distraction osteogenesis.

Original languageEnglish (US)
Pages (from-to)124-130
Number of pages7
JournalJournal of Bone and Joint Surgery - Series A
Volume85
Issue numberSUPPL. 3
StatePublished - Aug 1 2003

Fingerprint

Bone Morphogenetic Protein 7
Distraction Osteogenesis
Osteogenesis
Osteotomy
Bone Density
External Fixators
Control Groups
Torque
Ambulatory Surgical Procedures
Femur
Injections
Bone Lengthening
Bone and Bones
Long Evans Rats
human BMP7 protein

ASJC Scopus subject areas

  • Surgery
  • Orthopedics and Sports Medicine

Cite this

Acceleration of regenerate ossification during distraction osteogenesis with recombinant human bone morphogenetic protein-7. / Mizumoto, Yoshihiko; Moseley, Timothy; Drews, Michael; Cooper, Virgil N.; Reddi, A Hari.

In: Journal of Bone and Joint Surgery - Series A, Vol. 85, No. SUPPL. 3, 01.08.2003, p. 124-130.

Research output: Contribution to journalArticle

Mizumoto, Yoshihiko ; Moseley, Timothy ; Drews, Michael ; Cooper, Virgil N. ; Reddi, A Hari. / Acceleration of regenerate ossification during distraction osteogenesis with recombinant human bone morphogenetic protein-7. In: Journal of Bone and Joint Surgery - Series A. 2003 ; Vol. 85, No. SUPPL. 3. pp. 124-130.
@article{543c77ed7d50413c843172bc1f63f8b7,
title = "Acceleration of regenerate ossification during distraction osteogenesis with recombinant human bone morphogenetic protein-7",
abstract = "Background: Bone-lengthening can be accomplished by means of distraction osteogenesis. In the present study, we examined the effect of a single dose of recombinant human bone morphogenetic protein-7 (rhBMP-7) on the rate of new-bone formation during distraction osteogenesis in the rat. Methods: Fourteen Long-Evans rats were randomized into two groups of seven rats each. An external fixator was applied to the left femur, and a transverse osteotomy was performed. One group was treated with rhBMP-7 in an aqueous solvent, and the other group received the solvent alone and served as the control. rhBMP-7 was administered on the day of surgery by means of a single injection into the osteotomy gap. Distraction was started seven days after surgery at a rate of 0.25 mm every twelve hours. Distraction was continued for twenty days, resulting in a total of 10 mm of lengthening. The regenerate was monitored with use of radiographs and bone-mineral-density measurements at the conclusion of distraction and at two and four weeks after the cessation of distraction. The lengthened femora were harvested, and biomechanical studies were carried out to determine the stiffness and maximum torque to failure. Results: Radiographs showed accelerated regenerate ossification in the BMP-7 group, with a larger amount of new bone compared with the control group. The bone-mineral-density values were dramatically enhanced on Day 20 in the BMP-7 group (103.6 ± 12.6 mg/cm2) compared with the control group (26.2 ± 15.1 mg/cm2). These differences continued to be greater at two and four weeks after the cessation of distraction. Normalized values of stiffness (percent stiffness) reached 76.5{\%} ± 5.4{\%} in the BMP-7 group, compared with 6.6{\%} ± 0.5{\%} in the control group. The percent maximum torque at failure was 81.1{\%} ± 1.2{\%} in the BMP-7 group, compared with 20.8{\%} ± 0.3{\%} in the control group. Conclusion: A single injection of rhBMP-7 at the time of osteotomy surgery stimulated the rate of regenerate ossification and increased bone-mineral density during distraction osteogenesis. The biomechanical properties of the newly formed bone were also increased by BMP-7 injection. Clinical Relevance: The present study demonstrates the benefits of rhBMP-7 on the acceleration of regenerate ossification and indicates its potential utility in clinical situations to reduce the treatment period with an external fixator during distraction osteogenesis.",
author = "Yoshihiko Mizumoto and Timothy Moseley and Michael Drews and Cooper, {Virgil N.} and Reddi, {A Hari}",
year = "2003",
month = "8",
day = "1",
language = "English (US)",
volume = "85",
pages = "124--130",
journal = "Journal of Bone and Joint Surgery - American Volume",
issn = "0021-9355",
publisher = "Journal of Bone and Joint Surgery Inc.",
number = "SUPPL. 3",

}

TY - JOUR

T1 - Acceleration of regenerate ossification during distraction osteogenesis with recombinant human bone morphogenetic protein-7

AU - Mizumoto, Yoshihiko

AU - Moseley, Timothy

AU - Drews, Michael

AU - Cooper, Virgil N.

AU - Reddi, A Hari

PY - 2003/8/1

Y1 - 2003/8/1

N2 - Background: Bone-lengthening can be accomplished by means of distraction osteogenesis. In the present study, we examined the effect of a single dose of recombinant human bone morphogenetic protein-7 (rhBMP-7) on the rate of new-bone formation during distraction osteogenesis in the rat. Methods: Fourteen Long-Evans rats were randomized into two groups of seven rats each. An external fixator was applied to the left femur, and a transverse osteotomy was performed. One group was treated with rhBMP-7 in an aqueous solvent, and the other group received the solvent alone and served as the control. rhBMP-7 was administered on the day of surgery by means of a single injection into the osteotomy gap. Distraction was started seven days after surgery at a rate of 0.25 mm every twelve hours. Distraction was continued for twenty days, resulting in a total of 10 mm of lengthening. The regenerate was monitored with use of radiographs and bone-mineral-density measurements at the conclusion of distraction and at two and four weeks after the cessation of distraction. The lengthened femora were harvested, and biomechanical studies were carried out to determine the stiffness and maximum torque to failure. Results: Radiographs showed accelerated regenerate ossification in the BMP-7 group, with a larger amount of new bone compared with the control group. The bone-mineral-density values were dramatically enhanced on Day 20 in the BMP-7 group (103.6 ± 12.6 mg/cm2) compared with the control group (26.2 ± 15.1 mg/cm2). These differences continued to be greater at two and four weeks after the cessation of distraction. Normalized values of stiffness (percent stiffness) reached 76.5% ± 5.4% in the BMP-7 group, compared with 6.6% ± 0.5% in the control group. The percent maximum torque at failure was 81.1% ± 1.2% in the BMP-7 group, compared with 20.8% ± 0.3% in the control group. Conclusion: A single injection of rhBMP-7 at the time of osteotomy surgery stimulated the rate of regenerate ossification and increased bone-mineral density during distraction osteogenesis. The biomechanical properties of the newly formed bone were also increased by BMP-7 injection. Clinical Relevance: The present study demonstrates the benefits of rhBMP-7 on the acceleration of regenerate ossification and indicates its potential utility in clinical situations to reduce the treatment period with an external fixator during distraction osteogenesis.

AB - Background: Bone-lengthening can be accomplished by means of distraction osteogenesis. In the present study, we examined the effect of a single dose of recombinant human bone morphogenetic protein-7 (rhBMP-7) on the rate of new-bone formation during distraction osteogenesis in the rat. Methods: Fourteen Long-Evans rats were randomized into two groups of seven rats each. An external fixator was applied to the left femur, and a transverse osteotomy was performed. One group was treated with rhBMP-7 in an aqueous solvent, and the other group received the solvent alone and served as the control. rhBMP-7 was administered on the day of surgery by means of a single injection into the osteotomy gap. Distraction was started seven days after surgery at a rate of 0.25 mm every twelve hours. Distraction was continued for twenty days, resulting in a total of 10 mm of lengthening. The regenerate was monitored with use of radiographs and bone-mineral-density measurements at the conclusion of distraction and at two and four weeks after the cessation of distraction. The lengthened femora were harvested, and biomechanical studies were carried out to determine the stiffness and maximum torque to failure. Results: Radiographs showed accelerated regenerate ossification in the BMP-7 group, with a larger amount of new bone compared with the control group. The bone-mineral-density values were dramatically enhanced on Day 20 in the BMP-7 group (103.6 ± 12.6 mg/cm2) compared with the control group (26.2 ± 15.1 mg/cm2). These differences continued to be greater at two and four weeks after the cessation of distraction. Normalized values of stiffness (percent stiffness) reached 76.5% ± 5.4% in the BMP-7 group, compared with 6.6% ± 0.5% in the control group. The percent maximum torque at failure was 81.1% ± 1.2% in the BMP-7 group, compared with 20.8% ± 0.3% in the control group. Conclusion: A single injection of rhBMP-7 at the time of osteotomy surgery stimulated the rate of regenerate ossification and increased bone-mineral density during distraction osteogenesis. The biomechanical properties of the newly formed bone were also increased by BMP-7 injection. Clinical Relevance: The present study demonstrates the benefits of rhBMP-7 on the acceleration of regenerate ossification and indicates its potential utility in clinical situations to reduce the treatment period with an external fixator during distraction osteogenesis.

UR - http://www.scopus.com/inward/record.url?scp=0042134623&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0042134623&partnerID=8YFLogxK

M3 - Article

C2 - 12925619

AN - SCOPUS:0042134623

VL - 85

SP - 124

EP - 130

JO - Journal of Bone and Joint Surgery - American Volume

JF - Journal of Bone and Joint Surgery - American Volume

SN - 0021-9355

IS - SUPPL. 3

ER -