Abstract
Background: Bone-lengthening can be accomplished by means of distraction osteogenesis. In the present study, we examined the effect of a single dose of recombinant human bone morphogenetic protein-7 (rhBMP-7) on the rate of new-bone formation during distraction osteogenesis in the rat. Methods: Fourteen Long-Evans rats were randomized into two groups of seven rats each. An external fixator was applied to the left femur, and a transverse osteotomy was performed. One group was treated with rhBMP-7 in an aqueous solvent, and the other group received the solvent alone and served as the control. rhBMP-7 was administered on the day of surgery by means of a single injection into the osteotomy gap. Distraction was started seven days after surgery at a rate of 0.25 mm every twelve hours. Distraction was continued for twenty days, resulting in a total of 10 mm of lengthening. The regenerate was monitored with use of radiographs and bone-mineral-density measurements at the conclusion of distraction and at two and four weeks after the cessation of distraction. The lengthened femora were harvested, and biomechanical studies were carried out to determine the stiffness and maximum torque to failure. Results: Radiographs showed accelerated regenerate ossification in the BMP-7 group, with a larger amount of new bone compared with the control group. The bone-mineral-density values were dramatically enhanced on Day 20 in the BMP-7 group (103.6 ± 12.6 mg/cm2) compared with the control group (26.2 ± 15.1 mg/cm2). These differences continued to be greater at two and four weeks after the cessation of distraction. Normalized values of stiffness (percent stiffness) reached 76.5% ± 5.4% in the BMP-7 group, compared with 6.6% ± 0.5% in the control group. The percent maximum torque at failure was 81.1% ± 1.2% in the BMP-7 group, compared with 20.8% ± 0.3% in the control group. Conclusion: A single injection of rhBMP-7 at the time of osteotomy surgery stimulated the rate of regenerate ossification and increased bone-mineral density during distraction osteogenesis. The biomechanical properties of the newly formed bone were also increased by BMP-7 injection. Clinical Relevance: The present study demonstrates the benefits of rhBMP-7 on the acceleration of regenerate ossification and indicates its potential utility in clinical situations to reduce the treatment period with an external fixator during distraction osteogenesis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 124-130 |
| Number of pages | 7 |
| Journal | Journal of Bone and Joint Surgery - Series A |
| Volume | 85 |
| Issue number | SUPPL. 3 |
| State | Published - Aug 1 2003 |
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ASJC Scopus subject areas
- Surgery
- Orthopedics and Sports Medicine
Cite this
Acceleration of regenerate ossification during distraction osteogenesis with recombinant human bone morphogenetic protein-7. / Mizumoto, Yoshihiko; Moseley, Timothy; Drews, Michael; Cooper, Virgil N.; Reddi, A Hari.
In: Journal of Bone and Joint Surgery - Series A, Vol. 85, No. SUPPL. 3, 01.08.2003, p. 124-130.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Acceleration of regenerate ossification during distraction osteogenesis with recombinant human bone morphogenetic protein-7
AU - Mizumoto, Yoshihiko
AU - Moseley, Timothy
AU - Drews, Michael
AU - Cooper, Virgil N.
AU - Reddi, A Hari
PY - 2003/8/1
Y1 - 2003/8/1
N2 - Background: Bone-lengthening can be accomplished by means of distraction osteogenesis. In the present study, we examined the effect of a single dose of recombinant human bone morphogenetic protein-7 (rhBMP-7) on the rate of new-bone formation during distraction osteogenesis in the rat. Methods: Fourteen Long-Evans rats were randomized into two groups of seven rats each. An external fixator was applied to the left femur, and a transverse osteotomy was performed. One group was treated with rhBMP-7 in an aqueous solvent, and the other group received the solvent alone and served as the control. rhBMP-7 was administered on the day of surgery by means of a single injection into the osteotomy gap. Distraction was started seven days after surgery at a rate of 0.25 mm every twelve hours. Distraction was continued for twenty days, resulting in a total of 10 mm of lengthening. The regenerate was monitored with use of radiographs and bone-mineral-density measurements at the conclusion of distraction and at two and four weeks after the cessation of distraction. The lengthened femora were harvested, and biomechanical studies were carried out to determine the stiffness and maximum torque to failure. Results: Radiographs showed accelerated regenerate ossification in the BMP-7 group, with a larger amount of new bone compared with the control group. The bone-mineral-density values were dramatically enhanced on Day 20 in the BMP-7 group (103.6 ± 12.6 mg/cm2) compared with the control group (26.2 ± 15.1 mg/cm2). These differences continued to be greater at two and four weeks after the cessation of distraction. Normalized values of stiffness (percent stiffness) reached 76.5% ± 5.4% in the BMP-7 group, compared with 6.6% ± 0.5% in the control group. The percent maximum torque at failure was 81.1% ± 1.2% in the BMP-7 group, compared with 20.8% ± 0.3% in the control group. Conclusion: A single injection of rhBMP-7 at the time of osteotomy surgery stimulated the rate of regenerate ossification and increased bone-mineral density during distraction osteogenesis. The biomechanical properties of the newly formed bone were also increased by BMP-7 injection. Clinical Relevance: The present study demonstrates the benefits of rhBMP-7 on the acceleration of regenerate ossification and indicates its potential utility in clinical situations to reduce the treatment period with an external fixator during distraction osteogenesis.
AB - Background: Bone-lengthening can be accomplished by means of distraction osteogenesis. In the present study, we examined the effect of a single dose of recombinant human bone morphogenetic protein-7 (rhBMP-7) on the rate of new-bone formation during distraction osteogenesis in the rat. Methods: Fourteen Long-Evans rats were randomized into two groups of seven rats each. An external fixator was applied to the left femur, and a transverse osteotomy was performed. One group was treated with rhBMP-7 in an aqueous solvent, and the other group received the solvent alone and served as the control. rhBMP-7 was administered on the day of surgery by means of a single injection into the osteotomy gap. Distraction was started seven days after surgery at a rate of 0.25 mm every twelve hours. Distraction was continued for twenty days, resulting in a total of 10 mm of lengthening. The regenerate was monitored with use of radiographs and bone-mineral-density measurements at the conclusion of distraction and at two and four weeks after the cessation of distraction. The lengthened femora were harvested, and biomechanical studies were carried out to determine the stiffness and maximum torque to failure. Results: Radiographs showed accelerated regenerate ossification in the BMP-7 group, with a larger amount of new bone compared with the control group. The bone-mineral-density values were dramatically enhanced on Day 20 in the BMP-7 group (103.6 ± 12.6 mg/cm2) compared with the control group (26.2 ± 15.1 mg/cm2). These differences continued to be greater at two and four weeks after the cessation of distraction. Normalized values of stiffness (percent stiffness) reached 76.5% ± 5.4% in the BMP-7 group, compared with 6.6% ± 0.5% in the control group. The percent maximum torque at failure was 81.1% ± 1.2% in the BMP-7 group, compared with 20.8% ± 0.3% in the control group. Conclusion: A single injection of rhBMP-7 at the time of osteotomy surgery stimulated the rate of regenerate ossification and increased bone-mineral density during distraction osteogenesis. The biomechanical properties of the newly formed bone were also increased by BMP-7 injection. Clinical Relevance: The present study demonstrates the benefits of rhBMP-7 on the acceleration of regenerate ossification and indicates its potential utility in clinical situations to reduce the treatment period with an external fixator during distraction osteogenesis.
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M3 - Article
C2 - 12925619
AN - SCOPUS:0042134623
VL - 85
SP - 124
EP - 130
JO - Journal of Bone and Joint Surgery - American Volume
JF - Journal of Bone and Joint Surgery - American Volume
SN - 0021-9355
IS - SUPPL. 3
ER -