Abstract
A rapid and simple magnetic particle-based immunoassay has been demonstrated in a capillary mixing system. Antibody-coated micrometer size superparamagnetic polystyrene (SPP) particles were used in an assay for rabbit IgG in a sandwich (noncompetitive) format. The kinetics of the assay was compared between a plate-based system and a single capillary tube. The interaction between the antigen (R-IgG) and the antibody (anti-R-IgG) that was carried by the SPP particles in a rotating capillary was tested under a stationary magnetic field. Competing magnetic and viscous drag forces helped to enhance the interaction between the analyte and the capture antibodies on the particles. The dimensionless Mason number (Mn) was employed to characterize the magnetic particle dynamics; a previously determined critical Mason number (Mnc) was employed as a guide to the appropriate experimental conditions of magnetic field strength and rotational speed of the capillary. The advantage of the rotating capillary system included a short assay time and a reduced reactive volume (20 μL). The results show that the immunoassay kinetics were improved by the formation of chains of the SPP particles for the conditions that corresponded to the critical Mason number.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 8317-8322 |
| Number of pages | 6 |
| Journal | Analytical Chemistry |
| Volume | 84 |
| Issue number | 19 |
| DOIs | |
| State | Published - Oct 2 2012 |
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ASJC Scopus subject areas
- Analytical Chemistry
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Accelerated immunoassays based on magnetic particle dynamics in a rotating capillary tube with stationary magnetic field. / Lee, Jun Tae; Sudheendra, L.; Kennedy, Ian M.
In: Analytical Chemistry, Vol. 84, No. 19, 02.10.2012, p. 8317-8322.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Accelerated immunoassays based on magnetic particle dynamics in a rotating capillary tube with stationary magnetic field
AU - Lee, Jun Tae
AU - Sudheendra, L.
AU - Kennedy, Ian M.
PY - 2012/10/2
Y1 - 2012/10/2
N2 - A rapid and simple magnetic particle-based immunoassay has been demonstrated in a capillary mixing system. Antibody-coated micrometer size superparamagnetic polystyrene (SPP) particles were used in an assay for rabbit IgG in a sandwich (noncompetitive) format. The kinetics of the assay was compared between a plate-based system and a single capillary tube. The interaction between the antigen (R-IgG) and the antibody (anti-R-IgG) that was carried by the SPP particles in a rotating capillary was tested under a stationary magnetic field. Competing magnetic and viscous drag forces helped to enhance the interaction between the analyte and the capture antibodies on the particles. The dimensionless Mason number (Mn) was employed to characterize the magnetic particle dynamics; a previously determined critical Mason number (Mnc) was employed as a guide to the appropriate experimental conditions of magnetic field strength and rotational speed of the capillary. The advantage of the rotating capillary system included a short assay time and a reduced reactive volume (20 μL). The results show that the immunoassay kinetics were improved by the formation of chains of the SPP particles for the conditions that corresponded to the critical Mason number.
AB - A rapid and simple magnetic particle-based immunoassay has been demonstrated in a capillary mixing system. Antibody-coated micrometer size superparamagnetic polystyrene (SPP) particles were used in an assay for rabbit IgG in a sandwich (noncompetitive) format. The kinetics of the assay was compared between a plate-based system and a single capillary tube. The interaction between the antigen (R-IgG) and the antibody (anti-R-IgG) that was carried by the SPP particles in a rotating capillary was tested under a stationary magnetic field. Competing magnetic and viscous drag forces helped to enhance the interaction between the analyte and the capture antibodies on the particles. The dimensionless Mason number (Mn) was employed to characterize the magnetic particle dynamics; a previously determined critical Mason number (Mnc) was employed as a guide to the appropriate experimental conditions of magnetic field strength and rotational speed of the capillary. The advantage of the rotating capillary system included a short assay time and a reduced reactive volume (20 μL). The results show that the immunoassay kinetics were improved by the formation of chains of the SPP particles for the conditions that corresponded to the critical Mason number.
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UR - http://www.scopus.com/inward/citedby.url?scp=84867091580&partnerID=8YFLogxK
U2 - 10.1021/ac301848q
DO - 10.1021/ac301848q
M3 - Article
C2 - 22931580
AN - SCOPUS:84867091580
VL - 84
SP - 8317
EP - 8322
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 19
ER -