Acanthamoeba migration in an electric field

Jolene Chang Rudell, Jing Gao, Yuxin Sun, Yaohui Sun, James Chodosh, Ivan Schwab, Min Zhao

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

PURPOSE. We investigated the in vitro response of Acanthamoeba trophozoites to electric fields (EFs). METHODS. Acanthamoeba castellanii were exposed to varying strengths of an EF. During EF exposure, cell migration was monitored using an inverted microscope equipped with a CCD camera and the SimplePCI 5.3 imaging system to capture time-lapse images. The migration of A. castellanii trophozoites was analyzed and quantified with ImageJ software. For analysis of cell migration in a three-dimensional culture system, Acanthamoeba trophozoites were cultured in agar, exposed to an EF, digitally video recorded, and analyzed at various Z focal planes. RESULTS. Acanthamoeba trophozoites move at random in the absence of an EF, but move directionally in response to an EF. Directedness in the absence of an EF is 0.08±0.01, while in 1200 mV/mm EF, directedness is significantly higher at -0.65±0.01 (P < 0.001). We find that the trophozoite migration response is voltage-dependent, with higher directionality with higher voltage application. Acanthamoeba move directionally in a three-dimensional (3D) agar system as well when exposed to an EF. CONCLUSIONS. Acanthamoeba trophozoites move directionally in response to an EF in a twodimensional and 3D culture system. Acanthamoeba trophozoite migration is also voltagedependent, with increased directionality with increasing voltage. This may provide new treatment modalities for Acanthamoeba keratitis.

Original languageEnglish (US)
Pages (from-to)4225-4233
Number of pages9
JournalInvestigative Ophthalmology and Visual Science
Volume54
Issue number6
DOIs
StatePublished - 2013

Fingerprint

Acanthamoeba
Trophozoites
Acanthamoeba castellanii
Agar
Cell Movement
Acanthamoeba Keratitis
Software

Keywords

  • Acanthamoeba
  • Directional cell migration
  • Electric fields

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Rudell, J. C., Gao, J., Sun, Y., Sun, Y., Chodosh, J., Schwab, I., & Zhao, M. (2013). Acanthamoeba migration in an electric field. Investigative Ophthalmology and Visual Science, 54(6), 4225-4233. https://doi.org/10.1167/iovs.13-11968

Acanthamoeba migration in an electric field. / Rudell, Jolene Chang; Gao, Jing; Sun, Yuxin; Sun, Yaohui; Chodosh, James; Schwab, Ivan; Zhao, Min.

In: Investigative Ophthalmology and Visual Science, Vol. 54, No. 6, 2013, p. 4225-4233.

Research output: Contribution to journalArticle

Rudell, JC, Gao, J, Sun, Y, Sun, Y, Chodosh, J, Schwab, I & Zhao, M 2013, 'Acanthamoeba migration in an electric field', Investigative Ophthalmology and Visual Science, vol. 54, no. 6, pp. 4225-4233. https://doi.org/10.1167/iovs.13-11968
Rudell JC, Gao J, Sun Y, Sun Y, Chodosh J, Schwab I et al. Acanthamoeba migration in an electric field. Investigative Ophthalmology and Visual Science. 2013;54(6):4225-4233. https://doi.org/10.1167/iovs.13-11968
Rudell, Jolene Chang ; Gao, Jing ; Sun, Yuxin ; Sun, Yaohui ; Chodosh, James ; Schwab, Ivan ; Zhao, Min. / Acanthamoeba migration in an electric field. In: Investigative Ophthalmology and Visual Science. 2013 ; Vol. 54, No. 6. pp. 4225-4233.
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N2 - PURPOSE. We investigated the in vitro response of Acanthamoeba trophozoites to electric fields (EFs). METHODS. Acanthamoeba castellanii were exposed to varying strengths of an EF. During EF exposure, cell migration was monitored using an inverted microscope equipped with a CCD camera and the SimplePCI 5.3 imaging system to capture time-lapse images. The migration of A. castellanii trophozoites was analyzed and quantified with ImageJ software. For analysis of cell migration in a three-dimensional culture system, Acanthamoeba trophozoites were cultured in agar, exposed to an EF, digitally video recorded, and analyzed at various Z focal planes. RESULTS. Acanthamoeba trophozoites move at random in the absence of an EF, but move directionally in response to an EF. Directedness in the absence of an EF is 0.08±0.01, while in 1200 mV/mm EF, directedness is significantly higher at -0.65±0.01 (P < 0.001). We find that the trophozoite migration response is voltage-dependent, with higher directionality with higher voltage application. Acanthamoeba move directionally in a three-dimensional (3D) agar system as well when exposed to an EF. CONCLUSIONS. Acanthamoeba trophozoites move directionally in response to an EF in a twodimensional and 3D culture system. Acanthamoeba trophozoite migration is also voltagedependent, with increased directionality with increasing voltage. This may provide new treatment modalities for Acanthamoeba keratitis.

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