Abundant expression of granzyme A, but not perforin, in granules of CD8+ T cells in GALT

Implications for immune control of HIV-1 infection

Barbara Shacklett, Catherine A. Cox, Máire F. Quigley, Christophe Kreis, Neil H. Stollman, Mark A. Jacobson, Jan Andersson, Johan K. Sandberg, Douglas F. Nixon

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Because GALT is a major portal of entry for HIV-1 and reservoir for viral replication, we hypothesized that an ineffective cellular immune response in intestinal mucosa might partially explain the failure of immune control in AIDS. In this study, we demonstrate that the vast majority of CD8+ T cells in rectal tissue, including HIV-1-specific cells, fail to express the cytolytic protein, perforin. However, rectal CD8+ T cells do express granzyme A, and are also capable of releasing IFN-γ upon stimulation with cognate peptide. Confocal microscopy showed that granzyme A was located in intracellular-granules in the absence of perforin. The majority of rectal CD8+ T cells exhibit an effector memory phenotype, expressing CD45RO but not CCR7. Quantitative real-time PCR analysis demonstrated that perforin RNA is expressed in rectal CD8+ T cells from healthy and HIV-1-positive individuals. In HIV-1-positive individuals, similar amounts of perforin RNA were detected in CD8+ T cells from rectal tissue and PBMC, despite a relative absence of perforin protein in rectal tissue. These findings demonstrate an important difference in perforin expression between CD8 + T cells in blood and mucosa. Furthermore, the relative absence of armed effector cells may serve to protect the integrity of rectal mucosa under normal conditions, but might also provide an early advantage to HIV-1 and other sexually transmitted viruses.

Original languageEnglish (US)
Pages (from-to)641-648
Number of pages8
JournalJournal of Immunology
Volume173
Issue number1
StatePublished - Jul 1 2004

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Granzymes
Perforin
HIV Infections
HIV-1
T-Lymphocytes
Mucous Membrane
RNA
Intestinal Mucosa
Confocal Microscopy
Cellular Immunity
Real-Time Polymerase Chain Reaction
Acquired Immunodeficiency Syndrome
Proteins
Viruses
Phenotype
Peptides

ASJC Scopus subject areas

  • Immunology

Cite this

Shacklett, B., Cox, C. A., Quigley, M. F., Kreis, C., Stollman, N. H., Jacobson, M. A., ... Nixon, D. F. (2004). Abundant expression of granzyme A, but not perforin, in granules of CD8+ T cells in GALT: Implications for immune control of HIV-1 infection. Journal of Immunology, 173(1), 641-648.

Abundant expression of granzyme A, but not perforin, in granules of CD8+ T cells in GALT : Implications for immune control of HIV-1 infection. / Shacklett, Barbara; Cox, Catherine A.; Quigley, Máire F.; Kreis, Christophe; Stollman, Neil H.; Jacobson, Mark A.; Andersson, Jan; Sandberg, Johan K.; Nixon, Douglas F.

In: Journal of Immunology, Vol. 173, No. 1, 01.07.2004, p. 641-648.

Research output: Contribution to journalArticle

Shacklett, B, Cox, CA, Quigley, MF, Kreis, C, Stollman, NH, Jacobson, MA, Andersson, J, Sandberg, JK & Nixon, DF 2004, 'Abundant expression of granzyme A, but not perforin, in granules of CD8+ T cells in GALT: Implications for immune control of HIV-1 infection', Journal of Immunology, vol. 173, no. 1, pp. 641-648.
Shacklett, Barbara ; Cox, Catherine A. ; Quigley, Máire F. ; Kreis, Christophe ; Stollman, Neil H. ; Jacobson, Mark A. ; Andersson, Jan ; Sandberg, Johan K. ; Nixon, Douglas F. / Abundant expression of granzyme A, but not perforin, in granules of CD8+ T cells in GALT : Implications for immune control of HIV-1 infection. In: Journal of Immunology. 2004 ; Vol. 173, No. 1. pp. 641-648.
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